PMC:7572125 / 2629-3709
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7572125","sourcedb":"PMC","sourceid":"7572125","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7572125","text":"In 2011, Tay et al. reported PTEN was modulated through competing endogenous RNAs (ceRNAs), which are protein-coding RNA transcripts that compete for microRNAs through common micoRNA response elements (MREs). Testing four candidate PTEN ceRNAs Tay and colleagues reported that for three of these candidate ceRNAS (SERINC1, VAPA, and CNOT6L) silencing the ceRNAs impacted the activity of a luciferase construct engineered with the 3’UTR of PTEN (Tay et al., 2011). Using the same reporter construct, overexpression of the 3’UTRs of these ceRNAs were reported to increase luciferase activity suggesting inhibition of PTEN 3’UTR was relieved (Tay et al., 2011). These effects were reported to be dependent on microRNAs since inhibition of PTEN protein expression when ceRNAs were depleted was abrogated when DICER, a key part of the microRNA machinery, was disrupted (Tay et al., 2011). Two ceRNAs, VAPA and CNOT6L, when depleted also resulted in increased cell proliferation and phosphorylation of AKT when depleted, which was attenuated when DICER was disrupted (Tay et al., 2011).","tracks":[{"project":"2_test","denotations":[{"id":"33073769-22000013-27525578","span":{"begin":457,"end":461},"obj":"22000013"},{"id":"33073769-22000013-27525579","span":{"begin":652,"end":656},"obj":"22000013"},{"id":"33073769-22000013-27525580","span":{"begin":877,"end":881},"obj":"22000013"},{"id":"33073769-22000013-27525581","span":{"begin":1074,"end":1078},"obj":"22000013"}],"attributes":[{"subj":"33073769-22000013-27525578","pred":"source","obj":"2_test"},{"subj":"33073769-22000013-27525579","pred":"source","obj":"2_test"},{"subj":"33073769-22000013-27525580","pred":"source","obj":"2_test"},{"subj":"33073769-22000013-27525581","pred":"source","obj":"2_test"}]},{"project":"MyTest","denotations":[{"id":"33073769-22000013-27525578","span":{"begin":457,"end":461},"obj":"22000013"},{"id":"33073769-22000013-27525579","span":{"begin":652,"end":656},"obj":"22000013"},{"id":"33073769-22000013-27525580","span":{"begin":877,"end":881},"obj":"22000013"},{"id":"33073769-22000013-27525581","span":{"begin":1074,"end":1078},"obj":"22000013"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"33073769-22000013-27525578","pred":"source","obj":"MyTest"},{"subj":"33073769-22000013-27525579","pred":"source","obj":"MyTest"},{"subj":"33073769-22000013-27525580","pred":"source","obj":"MyTest"},{"subj":"33073769-22000013-27525581","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93bf","default":true},{"id":"MyTest","color":"#93d9ec"}]}]}}