PMC:7572125 / 15757-17105 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7572125","sourcedb":"PMC","sourceid":"7572125","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7572125","text":"Luciferase activity in DU145 cells co-transfected with 3’UTR of PTEN ceRNAs and a luciferase-PTEN 3’UTR reporter construct.\nDU145 cells were transfected with a luciferase reporter with a fragment of the 3’UTR of PTEN. Cells were also co-transfected with empty vector (EV) or plasmids that express the 3’UTR of SERINC1 (SER 3’U), VAPA (VAPA 3’U1 and VAPA 3’U2), CNOT6L (CNOT 3’U1 and CNOT 3’U2), or PTEN (PTEN 3’U). Cells were harvested 72 hr later for luciferase activity. Relative luminescence unit (RLU) is presented for each condition relative to the EV condition. Means reported and error bars represent SD from six independent biological repeats. Two-sample t-test of RLU values between SER 3’U and EV: t(10) = 3.32, uncorrected p=0.0077 with a priori Bonferroni adjusted significance threshold of 0.0083, Bonferroni corrected p=0.046; VAPA 3’U1 and EV: t(10) = 3.13, uncorrected p=0.011, Bonferroni corrected p=0.064; VAPA 3’U2 and EV: t(10) = 4.83, uncorrected p=6.90×10−4, Bonferroni corrected p=0.0041; CNOT 3’U1 and EV: t(10) = 4.42, uncorrected p=0.0013, Bonferroni corrected p=0.0078; CNOT 3’U2 and EV: t(7.1) = 5.09, uncorrected p=0.0014, Bonferroni corrected p=0.0082; PTEN 3’U and EV: t(5.6) = 7.50, uncorrected p=3.99×10−4, Bonferroni corrected p=0.0024. Additional details for this experiment can be found at https://osf.io/mryvq/.","divisions":[{"label":"title","span":{"begin":0,"end":123}}],"tracks":[]}