PMC:7572125 / 11053-12196 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7572125","sourcedb":"PMC","sourceid":"7572125","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7572125","text":"DU145 cells were transfected with a luciferase reporter with a fragment of the 3’UTR of PTEN. Cells were also co-transfected with non-targeting control siRNA (siNC) or siRNA plasmids targeting SERINC1 (siSER), ZNF460 (siZNF), VAPA (siVAPA), CNOT6L (siCNO), or PTEN (siPTEN). Cells were harvested 72 hr later for luciferase activity. Relative luminescence unit (RLU) is presented for each condition relative to the siNC condition. Means reported and error bars represent SD from four independent biological repeats. Two-sample t-test of RLU values between siNC and siSER: t(6) = 0.177, uncorrected p=0.866 with a priori Bonferroni adjusted significance threshold of 0.01, Bonferroni corrected p\u003e0.99; siNC and siZNF: t(6) = 0.899, uncorrected p=0.403, Bonferroni corrected p\u003e0.99; siNC and siVAPA: t(6) = 0.225, uncorrected p=0.829, Bonferroni corrected p\u003e0.99; siNC and siCNO: t(6) = 0.426, uncorrected p=0.685, Bonferroni corrected p\u003e0.99; Wilcoxon-Mann-Whitney test of RLU values between siNC and siPTEN: U = 16, uncorrected p=0.029, Bonferroni corrected p=0.143. Additional details for this experiment can be found at https://osf.io/spv4f/.","tracks":[]}