PMC:7571312 / 127911-128804 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7571312","sourcedb":"PMC","sourceid":"7571312","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7571312","text":"Crystals of compound 28 bound to SARS1/CoV-1 DC2 protease were produced via cocrystallization. SARS1/CoV-1 DC2 protease at 10.00 mg/mL was incubated with a 3-fold molar excess of compound 28 for 18 h at 4 °C. The complex was then passed through a 0.45 μM cellulose acetate spin filter and set up for crystallization using an NT-8 crystallization robot (Formulatrix). Using MRC-2 crystallization plates, wells containing 40 μL of 10% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M sodium potassium phosphate pH 6.2, and 10 mM TCEP were dispensed, and then sitting drops consisting of 0.3 μL protein were set up against a 0.3 μL well buffer. Crystallization plates were incubated at 21 °C, and rectangular crystals measuring 0.1 × 0.25 × 0.25 mm3 grew within 24 h. 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