PMC:7571312 / 121054-122470 JSONTXT

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LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T99 58-62 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T100 159-163 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T101 220-224 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T102 654-658 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T103 857-861 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T96 16-24 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T97 89-90 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T98 196-204 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T99 254-255 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T100 288-295 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T101 364-365 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T102 376-383 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T103 425-432 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T104 487-488 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T105 524-525 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T106 556-563 http://purl.obolibrary.org/obo/OBI_0000968 denotes Devices
T107 582-588 http://purl.obolibrary.org/obo/SO_0000418 denotes signal
T108 632-633 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T109 779-786 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T110 947-955 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T111 1037-1045 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T112 1051-1052 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T113 1090-1098 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T114 1134-1135 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T115 1386-1394 http://purl.obolibrary.org/obo/CLO_0001658 denotes Activity

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T70486 288-295 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T36781 376-383 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T53887 425-432 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T22935 619-621 Chemical denotes PF http://purl.obolibrary.org/obo/CHEBI_74795|http://purl.obolibrary.org/obo/CHEBI_90284
T29474 641-650 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T14079 692-698 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T17562 715-719 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T5538 720-723 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T32012 741-745 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T47682 752-756 Chemical denotes EDTA http://purl.obolibrary.org/obo/CHEBI_42191|http://purl.obolibrary.org/obo/CHEBI_64755
T12616 763-767 Chemical denotes TCEP http://purl.obolibrary.org/obo/CHEBI_63213
T32778 779-786 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T46679 1395-1399 Chemical denotes Base http://purl.obolibrary.org/obo/CHEBI_18282|http://purl.obolibrary.org/obo/CHEBI_22695

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T652 0-154 Sentence denotes The proteolytic activity of the main protease, 3CLpro, of SARS CoV-2 was monitored using a continuous fluorescence resonance energy-transfer (FRET) assay.
T653 155-565 Sentence denotes The SARS CoV-2 3CLpro assay measures the activity of full-length SARS CoV-2 3CL protease to cleave a synthetic fluorogenic substrate peptide with the following sequence DABCYL-KTSAVLQ-SGFRKME-EDANS modeled on a consensus peptide.50 The fluorescence of the cleaved EDANS peptide (excitation 340 nm/emission 490 nm) is measured using a fluorescence intensity protocol on a Flexstation reader (Molecular Devices).
T654 566-672 Sentence denotes The fluorescent signal is reduced in the presence of PF-00835231, a potent inhibitor of SARS CoV-2 3CLpro.
T655 673-797 Sentence denotes The assay reaction buffer contained 20 mM Tris–HCl (pH 7.3), 100 nM NaCl, 1 mM EDTA, 5 mM TCEP, and 25 μM peptide substrate.
T656 798-924 Sentence denotes Enzyme reactions were initiated with the addition of 15 nM SARS CoV-2 3CL protease and allowed to proceed for 60 min at 23 °C.
T657 925-1100 Sentence denotes Percent inhibition or activity was calculated based on control wells containing no compound (0% inhibition/100% activity) and a control compound (100% inhibition/0% activity).
T658 1101-1189 Sentence denotes IC50 values were generated using a four-parameter fit model using ABASE software (IDBS).
T659 1190-1416 Sentence denotes Ki values were fit to the Morrison equation with the enzyme concentration parameter fixed to 15 nM, the Km parameter fixed to 14 μM, and the substrate concentration parameter fixed to 25 μM using Activity Base software (IDBS).

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
2119 58-68 Species denotes SARS CoV-2 Tax:2697049
2120 159-169 Species denotes SARS CoV-2 Tax:2697049
2121 220-230 Species denotes SARS CoV-2 Tax:2697049
2122 654-664 Species denotes SARS CoV-2 Tax:2697049
2123 857-867 Species denotes SARS CoV-2 Tax:2697049
2124 715-723 Chemical denotes Tris–HCl
2125 741-745 Chemical denotes NaCl MESH:D012965
2126 752-756 Chemical denotes EDTA MESH:D004492
2127 763-767 Chemical denotes TCEP MESH:C080938

2_test

Id Subject Object Predicate Lexical cue
33054210-17397958-61913461 384-386 17397958 denotes 50