PMC:7571312 / 121010-122470 JSONTXT

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LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T98 0-4 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T99 102-106 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T100 203-207 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T101 264-268 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T102 698-702 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T103 901-905 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T96 60-68 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T97 133-134 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T98 240-248 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T99 298-299 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T100 332-339 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T101 408-409 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T102 420-427 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T103 469-476 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T104 531-532 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T105 568-569 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T106 600-607 http://purl.obolibrary.org/obo/OBI_0000968 denotes Devices
T107 626-632 http://purl.obolibrary.org/obo/SO_0000418 denotes signal
T108 676-677 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T109 823-830 http://purl.obolibrary.org/obo/PR_000018263 denotes peptide
T110 991-999 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T111 1081-1089 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T112 1095-1096 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T113 1134-1142 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity
T114 1178-1179 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T115 1430-1438 http://purl.obolibrary.org/obo/CLO_0001658 denotes Activity

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T70486 332-339 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T36781 420-427 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T53887 469-476 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T22935 663-665 Chemical denotes PF http://purl.obolibrary.org/obo/CHEBI_74795|http://purl.obolibrary.org/obo/CHEBI_90284
T29474 685-694 Chemical denotes inhibitor http://purl.obolibrary.org/obo/CHEBI_35222
T14079 736-742 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T17562 759-763 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T5538 764-767 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T32012 785-789 Chemical denotes NaCl http://purl.obolibrary.org/obo/CHEBI_26710
T47682 796-800 Chemical denotes EDTA http://purl.obolibrary.org/obo/CHEBI_42191|http://purl.obolibrary.org/obo/CHEBI_64755
T12616 807-811 Chemical denotes TCEP http://purl.obolibrary.org/obo/CHEBI_63213
T32778 823-830 Chemical denotes peptide http://purl.obolibrary.org/obo/CHEBI_16670
T46679 1439-1443 Chemical denotes Base http://purl.obolibrary.org/obo/CHEBI_18282|http://purl.obolibrary.org/obo/CHEBI_22695

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T651 0-43 Sentence denotes SARS CoV-2 Protease FRET Assay and Analysis
T652 44-198 Sentence denotes The proteolytic activity of the main protease, 3CLpro, of SARS CoV-2 was monitored using a continuous fluorescence resonance energy-transfer (FRET) assay.
T653 199-609 Sentence denotes The SARS CoV-2 3CLpro assay measures the activity of full-length SARS CoV-2 3CL protease to cleave a synthetic fluorogenic substrate peptide with the following sequence DABCYL-KTSAVLQ-SGFRKME-EDANS modeled on a consensus peptide.50 The fluorescence of the cleaved EDANS peptide (excitation 340 nm/emission 490 nm) is measured using a fluorescence intensity protocol on a Flexstation reader (Molecular Devices).
T654 610-716 Sentence denotes The fluorescent signal is reduced in the presence of PF-00835231, a potent inhibitor of SARS CoV-2 3CLpro.
T655 717-841 Sentence denotes The assay reaction buffer contained 20 mM Tris–HCl (pH 7.3), 100 nM NaCl, 1 mM EDTA, 5 mM TCEP, and 25 μM peptide substrate.
T656 842-968 Sentence denotes Enzyme reactions were initiated with the addition of 15 nM SARS CoV-2 3CL protease and allowed to proceed for 60 min at 23 °C.
T657 969-1144 Sentence denotes Percent inhibition or activity was calculated based on control wells containing no compound (0% inhibition/100% activity) and a control compound (100% inhibition/0% activity).
T658 1145-1233 Sentence denotes IC50 values were generated using a four-parameter fit model using ABASE software (IDBS).
T659 1234-1460 Sentence denotes Ki values were fit to the Morrison equation with the enzyme concentration parameter fixed to 15 nM, the Km parameter fixed to 14 μM, and the substrate concentration parameter fixed to 25 μM using Activity Base software (IDBS).

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
2109 0-10 Species denotes SARS CoV-2 Tax:2697049
2119 102-112 Species denotes SARS CoV-2 Tax:2697049
2120 203-213 Species denotes SARS CoV-2 Tax:2697049
2121 264-274 Species denotes SARS CoV-2 Tax:2697049
2122 698-708 Species denotes SARS CoV-2 Tax:2697049
2123 901-911 Species denotes SARS CoV-2 Tax:2697049
2124 759-767 Chemical denotes Tris–HCl
2125 785-789 Chemical denotes NaCl MESH:D012965
2126 796-800 Chemical denotes EDTA MESH:D004492
2127 807-811 Chemical denotes TCEP MESH:C080938

2_test

Id Subject Object Predicate Lexical cue
33054210-17397958-61913461 428-430 17397958 denotes 50