PMC:7558333 / 6785-7210 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T17","span":{"begin":215,"end":218},"obj":"Body_part"}],"attributes":[{"id":"A17","pred":"fma_id","subj":"T17","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T45","span":{"begin":91,"end":99},"obj":"Disease"}],"attributes":[{"id":"A45","pred":"mondo_id","subj":"T45","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T40","span":{"begin":248,"end":253},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T41","span":{"begin":270,"end":271},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T42","span":{"begin":288,"end":294},"obj":"http://purl.obolibrary.org/obo/OBI_0000968"},{"id":"T43","span":{"begin":328,"end":329},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T44","span":{"begin":356,"end":357},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T4","span":{"begin":152,"end":157},"obj":"http://purl.obolibrary.org/obo/GO_0019835"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}

{"project":"LitCovid-sentences","denotations":[{"id":"T52","span":{"begin":0,"end":304},"obj":"Sentence"},{"id":"T53","span":{"begin":305,"end":425},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}

{"project":"LitCovid-PubTator","denotations":[{"id":"154","span":{"begin":91,"end":101},"obj":"Species"},{"id":"155","span":{"begin":328,"end":340},"obj":"Species"},{"id":"156","span":{"begin":367,"end":378},"obj":"Species"},{"id":"157","span":{"begin":148,"end":157},"obj":"Disease"}],"attributes":[{"id":"A154","pred":"tao:has_database_id","subj":"154","obj":"Tax:2697049"},{"id":"A155","pred":"tao:has_database_id","subj":"155","obj":"Tax:746128"},{"id":"A156","pred":"tao:has_database_id","subj":"156","obj":"Tax:746128"},{"id":"A157","pred":"tao:has_database_id","subj":"157","obj":"MESH:D015275"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}

{"project":"2_test","denotations":[{"id":"32664423-29248586-60101698","span":{"begin":418,"end":420},"obj":"29248586"},{"id":"32664423-14766869-60101699","span":{"begin":421,"end":423},"obj":"14766869"}],"text":"For molecular detection, 200 µL of plain fluidified respiratory sample underwent immediate SARS-CoV-2 inactivation by heating at 56 °C overnight in ATL Lysis buffer (Qiagen, Saint-Quentin Fallavier, France), before DNA extraction using the EZ1 DSP virus kit (Qiagen) on a EZ1 Advanced XL device (Qiagen). Molecular detection of A. fumigatus was done using a 28S rDNA Aspergillus-targeted PCR, as previously published [11,12]."}