PMC:7556614 / 9883-11664 JSONTXT

{"project":"2_test","denotations":[{"id":"33057878-30766442-28008","span":{"begin":185,"end":187},"obj":"30766442"}],"text":"Optimisation of Formulation\n\nDrug Entrapment Efficiency Analysis\nAmount of encapsulated ACV in nanoparticles was identified indirectly in high-performance liquid chromatography (HPLC) (24). Agilent 1260 Series HPLC system fitted with a photodiode array detector (DAD), and use Thermo Fisher Syncronis C-18 (5-μm particle size, 150 × 4.60 mm diameter) column. A fixed injection volume of 20 μl per sample, a column temperature of 25°C, and a wavelength of 254 nm were set. The mobile phase used was acetonitrile and pH 6.74 water (10:90, v/v), which was set at an isocratic flow rate of 0.5 ml/min for 10-min run time. Equation (1) showed a calibration curve that fit over the range 100–500 μg/ml with a correlation coefficient of r2 = 0.99990.1 y=117.88803x+43.82506where y represented the peak area in a milli-absorbance unit (mAu) and x represented the ACV concentration (mg/ml).\nIn evaluating the entrapment efficiency (EE), PALNs prepared were stored in − 80°C freezer for 24 h. Then PALNs were thawed and isolated by centrifugation at 48,000×g at 4°C for 20 min. The supernatants consisted of the free drug were:2 EE%=Mtotal–Mfree/Mtotal100%where EE was the entrapment efficiency, and Mtotal and Mfree were a total mass of ACV added and free ACV in supernatant respectively.\n\nZeta Potential Measurement Particle Size and Poly-Dispersity Index\nParticle size, Poly-Dispersity Index (PDI), and zeta potential were determined by DelsaMax PRO Zeta Potential Dynamic Light Scattering Analyzer (Beckman Coulter, USA). To measure the size and zeta potential, 0.1 ml of prepared particles was diluted with 2.0 ml of deionised water loaded in flow cell and scanned for particles. The measurement was done in a 173° scattering angle. The experiment was done with a setting of 25 ± 0.5°C."}