PMC:7556614 / 17346-18417 JSONTXT

{"project":"2_test","denotations":[{"id":"33057878-28480807-28011","span":{"begin":64,"end":66},"obj":"28480807"}],"text":"Ex vivo study was performed according to an established method (25) with modification. The study was done using New Zealand white rabbit of age 5–6 months. The rabbit intestine sac was cleaned with cold saline. It was cut into several 9.5-cm-long segments. The intestine segment was tied up securely with thread at one end, followed by the introduction of 2 mL of optimised formulation with 1 mg/mL of ACV concentration into the intestinal opening. The other end was then tied up securely with thread. The rabbit intestine sac containing the formulation was kept in sink condition in a beaker containing 80 mL of pH 7.4 PBS with 1% m/v sodium lauryl sulphate (SLS) at 37°C as releasing medium. The beaker was closed with cover to prevent evaporation of releasing medium. One millilitre of the sample was taken at 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h, and 10 h in triplicate, and an equal amount of fresh releasing medium was replenished. Samples were analysed with HPLC method as described in section E, and Eq. (3) was used for calculation of ACV concentration in the samples."}