PMC:7556614 / 17305-19531
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7556614","sourcedb":"PMC","sourceid":"7556614","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7556614","text":"Study of Ex vivo Intestinal Permeability\nEx vivo study was performed according to an established method (25) with modification. The study was done using New Zealand white rabbit of age 5–6 months. The rabbit intestine sac was cleaned with cold saline. It was cut into several 9.5-cm-long segments. The intestine segment was tied up securely with thread at one end, followed by the introduction of 2 mL of optimised formulation with 1 mg/mL of ACV concentration into the intestinal opening. The other end was then tied up securely with thread. The rabbit intestine sac containing the formulation was kept in sink condition in a beaker containing 80 mL of pH 7.4 PBS with 1% m/v sodium lauryl sulphate (SLS) at 37°C as releasing medium. The beaker was closed with cover to prevent evaporation of releasing medium. One millilitre of the sample was taken at 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h, and 10 h in triplicate, and an equal amount of fresh releasing medium was replenished. Samples were analysed with HPLC method as described in section E, and Eq. (3) was used for calculation of ACV concentration in the samples.\nDrug intestinal permeability profile of optimised PALN formulation was plotted with a cumulative amount of ACV permeated into releasing medium against time. Cumulative percentage of drug permeated was calculate with Eq. (5)5 DP%=Mmedium/Mtotal100%where DP was the cumulative percentage of drug permeated and Mmedium was the mass of acyclovir in releasing medium.\nApparent permeability of the formulation as a function of the mucosal surface area was calculated by Eq. (6). In contrast, Eqs. (7) and (8) were used to calculate apparent permeability coefficient and steady-state flux, and Jss of acyclovir in formulation, respectively.6 Apparentpermeabilitymgcm−2=Mmedium/MucosalSurfaceAreacm27 Pappcms−1=dQ/dt1/AC0\nwhere Papp was the apparent permeability coefficient, dQ/dt was the slope for the graph of the cumulative amount of ACV permeated against time in unit of mg/h, A is the surface area of intestinal tissue where the permeation of nanoparticles occurred in the unit of cm2, and C0 gave an initial concentration of ACV in the intestinal sac that was 1 mg/mL (equivalent to 1 mg/cm3).8 Jssmgcm−2s−1=PappC0","divisions":[{"label":"title","span":{"begin":0,"end":40}},{"label":"p","span":{"begin":41,"end":1112}},{"label":"p","span":{"begin":1113,"end":1475}},{"label":"label","span":{"begin":1336,"end":1337}},{"label":"p","span":{"begin":1476,"end":1826}},{"label":"label","span":{"begin":1746,"end":1747}},{"label":"label","span":{"begin":1804,"end":1805}},{"label":"label","span":{"begin":2205,"end":2206}}],"tracks":[{"project":"2_test","denotations":[{"id":"33057878-28480807-28011","span":{"begin":105,"end":107},"obj":"28480807"}],"attributes":[{"subj":"33057878-28480807-28011","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec9f93","default":true}]}]}}