PMC:7554011 / 10853-12390 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7554011","sourcedb":"PMC","sourceid":"7554011","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7554011","text":"Dissolution studies were performed with a mini-paddle apparatus (Agilent Technologies 708-DS apparatus configured with TruAlign 200 mL vessels and electropolished stainless-steel mini-paddles; Agilent, USA). Experiments were conducted at 37°C, and agitation rate was set to 50 revolutions per minute (rpm). A two-stage approach was followed: gastric conditions were simulated for 1 h (Pi-FaSSGF pH 1.6; total volume with sample: 100 mL), followed by intestinal simulated conditions (FaSSIF-V2 pH 6.5 or Pi-FeSSIF pH 5.8; final volume: 200 mL), for 3 h. Sample collection took place at 5, 15, 30, 45, 60, 75, 90, 120, 180 and 240 min. Samples of 2 mL were withdrawn (with volume replacement with the corresponding media), using a 2-mL glass syringe (Fortuna Optima® fitted with a stainless tubing) through a cannula fitted with a full flow filter (10 μm). All experiments were performed without direct light exposure to avoid photodegradation of montelukast (21). After collection, samples were filtered through a GF/D filter (2.7 μm), treated, placed into amber HPLC vials and injected into the HPLC. Treatment was as follows: 1000 μL of acetonitrile was added to 500 μL of the filtered sample, the mixture was vortexed (HTZ, UK) for 1 min and centrifuged (8000 rpm, 15 min, 4°C) (Beckman Coulter J2-MC centrifuge, UK) and the supernatant was filtered through a RC filter (0.45 μm). The pH of the media was measured at the end of each experiment to ensure the pH shift had been successful and that the vehicle did not alter the media pH.","tracks":[{"project":"2_test","denotations":[{"id":"33051713-15497676-26793","span":{"begin":958,"end":960},"obj":"15497676"}],"attributes":[{"subj":"33051713-15497676-26793","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93d3","default":true}]}]}}