| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T57 |
0-23 |
Sentence |
denotes |
(iv) 3D reconstruction. |
| T58 |
24-177 |
Sentence |
denotes |
We then performed a cryoSPARC non-uniform 3D reconstruction, followed by a local angular refinement using RELION with a 3D mask (Zivanov et al., 2018 ▸). |
| T59 |
178-438 |
Sentence |
denotes |
Particle images were subjected to CTF refinement and Bayesian polishing (Zivanov et al., 2018 ▸), before performing another two rounds of CTF refinement and local angular refinement in RELION, where we improved the resolution versus the first local refinement. |
| T60 |
439-499 |
Sentence |
denotes |
Finally, we performed a non-uniform refinement in cryoSPARC. |
| T61 |
500-623 |
Sentence |
denotes |
The reported nominal resolution of 2.96 Å is based on the gold-standard Fourier shell correlation (FSC) of 0.143 criterion. |
| T62 |
624-855 |
Sentence |
denotes |
Actually, by using Xmipp Highres (Sorzano et al., 2018 ▸) we could improve the resolution to 2.2 Å in the central region of the volume (the region that is not flexible), but at the expense of reducing it more in the flexible areas. |
