PMC:7551562 / 32820-34719 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7551562","sourcedb":"PMC","sourceid":"7551562","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7551562","text":"Cocktail Formulation\nThe bioremediation cocktail was formulated to offer the benefits of bioaugmentation (consortia of four high throughputs hydrocarbon-degrading bacteria selected through hydrocarbon degradation screening study) and biostimulation (three agrowaste residues that are sources of N:P:K selected from OVAT nutrient and concentration range study).\nThe following bacterial isolates Pseudomonas fluorescens, Achromobacter agilis, Bacillus thuringiensis, and Staphylococcus lentus were selected for their high hydrocarbon-degrading capabilities and corn steep liquor for Nitrogen and poultry droppings for Phosphorus were selected for their ability to support mass cultivation of the selected isolates. However, plantain peel char powder was not selected just for its potassium content benefit to microbial activities, which experimental OVAT values both statistically and analytically indicate a constant nutrient value across all bacterial isolates. Plantain peel char was selected more for its biochar influence on bioremediation. As the only agrowaste residue charred, it was an ideal mix for the cocktail formulation.\nThe laboratory results were analysed using both ANOVA and the Monod model for bacterial growth dynamics to select the optimum nutrients concentration range and results were inputted into the RSM-DX program. The Random Surface Model has the following as the independent variable or factors; Nitrogen, Phosphorus and Inoculum Size. The result of the RSM-DX program was a randomization of nutrients and microbial consortia in ratios designed to formulate a bioremediation cocktail mixture (see Table 2 for cocktail ratio). Each ratio of the nutrient and microbial consortia is a unique portion of the biococktail and in each portion a 2.5g of plantain peels char is added to make the final cocktail mixture before it is applied to the experimental polluted soil sample.","divisions":[{"label":"title","span":{"begin":0,"end":20}},{"label":"p","span":{"begin":21,"end":360}},{"label":"p","span":{"begin":361,"end":1132}}],"tracks":[]}