PMC:7551562 / 25773-26901 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7551562","sourcedb":"PMC","sourceid":"7551562","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7551562","text":"Determination of Phosphate- Phosphorus (Ascorbic Method)\nSoil sample (5g) was extracted with 50ml of 2.5% acetic acid (71251, Supelco). The extract was filtered into a 250ml conical flask. A blank and standard phosphate ion concentration (103935, Supelco) ranging from 0.0001 – 0.0007 was prepared and 0.8ml already prepared combined reagent of molybdate (69888, Supelco) and ascorbic acid (100468, Supelco) was added respectively. The bluish colour developed within a 30 mins interval and optical densitity was read at 840nm using a spectrophotometer (Thermospectronic, Genesys 20 series). Vital settings as recommended for the instrument employed were programmed. The extracted sample volume developed was also read at the same wavelength. The concentration of the phosphate ion in the sample was extrapolated from the standard graph plotted with the value from the standard phosphate ion range.\nPhosphorus ion in the soil was obtained by multiplying the phosphate ion by factor of 0.3262 (factor of 0.3262 was derived from the ratio of molecular weight of the total phosphorus to the molecular weight of the total phosphate).","divisions":[{"label":"title","span":{"begin":0,"end":56}},{"label":"p","span":{"begin":57,"end":897}}],"tracks":[]}