PMC:7551562 / 10324-11883 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7551562","sourcedb":"PMC","sourceid":"7551562","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7551562","text":"In-Vitro screening for hydrocarbon degradation potential of bacterial isolates\nCrude oil biodegradation screening of the organism was determined by the method given by Shekhar et al. (2015). In vitro hydrocarbon degradation potential was determined using 18h old bacterial inoculum (1ml) transferred into 98ml Bushnell-Hass medium (Lab M, China) at pH 7.0 and was supplemented with 1ml of Bonny light crude oil as the carbon source. It was incubated at 37°C at 170 rpm in shaker incubator for a period of 7days. The hydrocarbon degradative potential of the bacterial isolates was both monitored by viable plate count and optical density (OD) method. The growth of the bacterium was measured by taking the optical density (OD) readings at 600nm for 7 days at regular 1-day intervals by a spectrophotometer, against the Bushnell Haas medium as blank. A corresponding 5ml aliquot sample was collected for 1ml serial dilution viable plate count and a corresponding daily reading of pH was taken and recorded. All experiments were performed in duplicate ( Olukunle et al., 2015; Dilmi et al., 2017). The total viable plate count versus incubation time for each bacterial isolates were plotted into growth model curve, and using the first-order growth rate equation, the growth kinetic parameters such as specific growth rate were calculated. The viable plate count and optical density results were also analysed using one-way ANOVA. Both the specific growth rate and ANOVA results were the basis for the selection of the four isolates for the cocktail formulation.","divisions":[{"label":"title","span":{"begin":0,"end":79}}],"tracks":[{"project":"2_test","denotations":[{"id":"33094269-26464607-29069","span":{"begin":1068,"end":1072},"obj":"26464607"}],"attributes":[{"subj":"33094269-26464607-29069","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#d8ec93","default":true}]}]}}