PMC:7544934 / 8199-9337 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
225	793-798	Gene	denotes	CD11b	Gene:16409
226	967-970	Gene	denotes	APC	Gene:324
227	978-983	Gene	denotes	CD11b	Gene:16409
228	0-4	Species	denotes	Mice	Tax:10090
229	787-792	Species	denotes	mouse	Tax:10090
230	123-126	Chemical	denotes	PBS	MESH:D007854
231	157-162	Chemical	denotes	HEPES	MESH:D006531
232	163-174	Chemical	denotes	DMEM buffer
233	36-46	Disease	denotes	epididymal	MESH:D004823

Id	Subject	Object	Predicate	Lexical cue
T67	0-86	Sentence	denotes	Mice were euthanized chemically and epididymal fat was processed for isolation of ATM.
T68	87-199	Sentence	denotes	1 gram of adipose fat was rinsed in PBS and minced to small pieces in HEPES-DMEM buffer containing 10 mg/ml BSA.
T69	200-313	Sentence	denotes	The suspension was centrifuged at 1000 g for 10 min and the resultant supernatant was pipette off to fresh tubes.
T70	314-560	Sentence	denotes	1 mg/ml of collagenase type-IV and 50 U/ml DNAse-II were added to this suspension and incubated at 37 °C for 45 mins with moderate shaking, filtered through 250-micron filter and the resultant solution was centrifuged again at 1000 g for 10 mins.
T71	561-615	Sentence	denotes	Floating cells contained adipocyte and pellet are SVC.
T72	616-729	Sentence	denotes	RBC lysis buffer was added gently to disrupt the sedimented pellets and centrifuged at 1000 g for 10 min at 4 °C.
T73	730-868	Sentence	denotes	Fat macrophage cells were isolated by using BD IMag anti-mouse CD11b + magnetic beads through positive selection under the magnetic field.
T74	869-1004	Sentence	denotes	The percentage purity of macrophage isolation was determined by FACSCANTO II flow cytometer using APC tagged CD11b monoclonal antibody.
T75	1005-1138	Sentence	denotes	The isolated macrophage was processed for RNA isolation, cDNA synthesis and various M1-M2 markers were evaluated using real-time PCR.