PMC:7537531 / 3389-4796 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T9","span":{"begin":649,"end":653},"obj":"Body_part"},{"id":"T10","span":{"begin":698,"end":703},"obj":"Body_part"},{"id":"T11","span":{"begin":803,"end":807},"obj":"Body_part"},{"id":"T12","span":{"begin":836,"end":840},"obj":"Body_part"},{"id":"T13","span":{"begin":1336,"end":1341},"obj":"Body_part"},{"id":"T14","span":{"begin":1369,"end":1374},"obj":"Body_part"}],"attributes":[{"id":"A9","pred":"fma_id","subj":"T9","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A10","pred":"fma_id","subj":"T10","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A11","pred":"fma_id","subj":"T11","obj":"http://purl.org/sig/ont/fma/fma256135"},{"id":"A12","pred":"fma_id","subj":"T12","obj":"http://purl.org/sig/ont/fma/fma256135"},{"id":"A13","pred":"fma_id","subj":"T13","obj":"http://purl.org/sig/ont/fma/fma9670"},{"id":"A14","pred":"fma_id","subj":"T14","obj":"http://purl.org/sig/ont/fma/fma68877"}],"text":"24 five‐ to seven‐week‐old female and male Chinese hamsters (Cricetulus griseus) were obtained via the German pet trade and kept in groups of six animals in enriched, individually ventilated cages. The hamsters had ad libidum access to food and water, and were allowed acclimatization to these conditions for seven days prior to SARS‐CoV‐2 infection. Cage temperatures and relative humidities were recorded daily and ranged from 23 to 24°C and from 50% to 65%, respectively. The animals were randomly distributed into two groups: SARS‐CoV‐2‐infected (n = 12; 1 × 105 plaque‐forming units (pfu) in a volume of 30 µl) and mock‐infected (n = 12; 30 µl cell culture supernatant from uninfected Vero E6 cells). The infection was performed exactly as previously described (Osterrieder et al., 2020). Baseline body weights of all hamsters and body temperatures of at least three hamsters per group were measured before infection and then recorded daily throughout the 14‐day experiment. Moreover, clinical signs of all animals were monitored twice daily throughout the experiment. On days 2, 3, 5 and 14 post‐infection (dpi), three randomly assigned hamsters of each group were euthanized by exsanguination under general anaesthesia as described (Nakamura et al., 2017). For virus titrations and RT‐qPCR and/or histopathological examinations, blood, bucco‐laryngeal swabs and lungs (left and right) were collected."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T6","span":{"begin":1336,"end":1341},"obj":"Body_part"}],"attributes":[{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"24 five‐ to seven‐week‐old female and male Chinese hamsters (Cricetulus griseus) were obtained via the German pet trade and kept in groups of six animals in enriched, individually ventilated cages. The hamsters had ad libidum access to food and water, and were allowed acclimatization to these conditions for seven days prior to SARS‐CoV‐2 infection. Cage temperatures and relative humidities were recorded daily and ranged from 23 to 24°C and from 50% to 65%, respectively. The animals were randomly distributed into two groups: SARS‐CoV‐2‐infected (n = 12; 1 × 105 plaque‐forming units (pfu) in a volume of 30 µl) and mock‐infected (n = 12; 30 µl cell culture supernatant from uninfected Vero E6 cells). The infection was performed exactly as previously described (Osterrieder et al., 2020). Baseline body weights of all hamsters and body temperatures of at least three hamsters per group were measured before infection and then recorded daily throughout the 14‐day experiment. Moreover, clinical signs of all animals were monitored twice daily throughout the experiment. On days 2, 3, 5 and 14 post‐infection (dpi), three randomly assigned hamsters of each group were euthanized by exsanguination under general anaesthesia as described (Nakamura et al., 2017). For virus titrations and RT‐qPCR and/or histopathological examinations, blood, bucco‐laryngeal swabs and lungs (left and right) were collected."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T35","span":{"begin":329,"end":333},"obj":"Disease"},{"id":"T36","span":{"begin":340,"end":349},"obj":"Disease"},{"id":"T37","span":{"begin":530,"end":534},"obj":"Disease"},{"id":"T38","span":{"begin":710,"end":719},"obj":"Disease"},{"id":"T39","span":{"begin":912,"end":921},"obj":"Disease"},{"id":"T40","span":{"begin":1102,"end":1111},"obj":"Disease"}],"attributes":[{"id":"A35","pred":"mondo_id","subj":"T35","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A36","pred":"mondo_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A37","pred":"mondo_id","subj":"T37","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A38","pred":"mondo_id","subj":"T38","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A39","pred":"mondo_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"id":"A40","pred":"mondo_id","subj":"T40","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"24 five‐ to seven‐week‐old female and male Chinese hamsters (Cricetulus griseus) were obtained via the German pet trade and kept in groups of six animals in enriched, individually ventilated cages. The hamsters had ad libidum access to food and water, and were allowed acclimatization to these conditions for seven days prior to SARS‐CoV‐2 infection. Cage temperatures and relative humidities were recorded daily and ranged from 23 to 24°C and from 50% to 65%, respectively. The animals were randomly distributed into two groups: SARS‐CoV‐2‐infected (n = 12; 1 × 105 plaque‐forming units (pfu) in a volume of 30 µl) and mock‐infected (n = 12; 30 µl cell culture supernatant from uninfected Vero E6 cells). The infection was performed exactly as previously described (Osterrieder et al., 2020). Baseline body weights of all hamsters and body temperatures of at least three hamsters per group were measured before infection and then recorded daily throughout the 14‐day experiment. Moreover, clinical signs of all animals were monitored twice daily throughout the experiment. On days 2, 3, 5 and 14 post‐infection (dpi), three randomly assigned hamsters of each group were euthanized by exsanguination under general anaesthesia as described (Nakamura et al., 2017). For virus titrations and RT‐qPCR and/or histopathological examinations, blood, bucco‐laryngeal swabs and lungs (left and right) were collected."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T44","span":{"begin":27,"end":33},"obj":"http://purl.obolibrary.org/obo/UBERON_0003100"},{"id":"T45","span":{"begin":38,"end":42},"obj":"http://purl.obolibrary.org/obo/UBERON_0003101"},{"id":"T46","span":{"begin":38,"end":42},"obj":"http://www.ebi.ac.uk/efo/EFO_0000970"},{"id":"T47","span":{"begin":51,"end":59},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10026"},{"id":"T48","span":{"begin":61,"end":79},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10029"},{"id":"T49","span":{"begin":146,"end":153},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T50","span":{"begin":202,"end":210},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10026"},{"id":"T51","span":{"begin":479,"end":486},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T52","span":{"begin":555,"end":560},"obj":"http://purl.obolibrary.org/obo/CLO_0001053"},{"id":"T53","span":{"begin":597,"end":598},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T54","span":{"begin":649,"end":653},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T55","span":{"begin":690,"end":703},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T56","span":{"begin":823,"end":831},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10026"},{"id":"T57","span":{"begin":872,"end":880},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10026"},{"id":"T58","span":{"begin":1012,"end":1019},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_33208"},{"id":"T59","span":{"begin":1085,"end":1089},"obj":"http://purl.obolibrary.org/obo/CLO_0001000"},{"id":"T60","span":{"begin":1143,"end":1151},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10026"},{"id":"T61","span":{"begin":1268,"end":1273},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T62","span":{"begin":1336,"end":1341},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T63","span":{"begin":1336,"end":1341},"obj":"http://www.ebi.ac.uk/efo/EFO_0000296"},{"id":"T64","span":{"begin":1369,"end":1374},"obj":"http://www.ebi.ac.uk/efo/EFO_0000934"}],"text":"24 five‐ to seven‐week‐old female and male Chinese hamsters (Cricetulus griseus) were obtained via the German pet trade and kept in groups of six animals in enriched, individually ventilated cages. The hamsters had ad libidum access to food and water, and were allowed acclimatization to these conditions for seven days prior to SARS‐CoV‐2 infection. Cage temperatures and relative humidities were recorded daily and ranged from 23 to 24°C and from 50% to 65%, respectively. The animals were randomly distributed into two groups: SARS‐CoV‐2‐infected (n = 12; 1 × 105 plaque‐forming units (pfu) in a volume of 30 µl) and mock‐infected (n = 12; 30 µl cell culture supernatant from uninfected Vero E6 cells). The infection was performed exactly as previously described (Osterrieder et al., 2020). Baseline body weights of all hamsters and body temperatures of at least three hamsters per group were measured before infection and then recorded daily throughout the 14‐day experiment. Moreover, clinical signs of all animals were monitored twice daily throughout the experiment. On days 2, 3, 5 and 14 post‐infection (dpi), three randomly assigned hamsters of each group were euthanized by exsanguination under general anaesthesia as described (Nakamura et al., 2017). For virus titrations and RT‐qPCR and/or histopathological examinations, blood, bucco‐laryngeal swabs and lungs (left and right) were collected."}

{"project":"LitCovid-sentences","denotations":[{"id":"T26","span":{"begin":0,"end":197},"obj":"Sentence"},{"id":"T27","span":{"begin":198,"end":350},"obj":"Sentence"},{"id":"T28","span":{"begin":351,"end":474},"obj":"Sentence"},{"id":"T29","span":{"begin":475,"end":705},"obj":"Sentence"},{"id":"T30","span":{"begin":706,"end":793},"obj":"Sentence"},{"id":"T31","span":{"begin":794,"end":979},"obj":"Sentence"},{"id":"T32","span":{"begin":980,"end":1073},"obj":"Sentence"},{"id":"T33","span":{"begin":1074,"end":1263},"obj":"Sentence"},{"id":"T34","span":{"begin":1264,"end":1407},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"24 five‐ to seven‐week‐old female and male Chinese hamsters (Cricetulus griseus) were obtained via the German pet trade and kept in groups of six animals in enriched, individually ventilated cages. The hamsters had ad libidum access to food and water, and were allowed acclimatization to these conditions for seven days prior to SARS‐CoV‐2 infection. Cage temperatures and relative humidities were recorded daily and ranged from 23 to 24°C and from 50% to 65%, respectively. The animals were randomly distributed into two groups: SARS‐CoV‐2‐infected (n = 12; 1 × 105 plaque‐forming units (pfu) in a volume of 30 µl) and mock‐infected (n = 12; 30 µl cell culture supernatant from uninfected Vero E6 cells). The infection was performed exactly as previously described (Osterrieder et al., 2020). Baseline body weights of all hamsters and body temperatures of at least three hamsters per group were measured before infection and then recorded daily throughout the 14‐day experiment. Moreover, clinical signs of all animals were monitored twice daily throughout the experiment. On days 2, 3, 5 and 14 post‐infection (dpi), three randomly assigned hamsters of each group were euthanized by exsanguination under general anaesthesia as described (Nakamura et al., 2017). For virus titrations and RT‐qPCR and/or histopathological examinations, blood, bucco‐laryngeal swabs and lungs (left and right) were collected."}

{"project":"LitCovid-PubTator","denotations":[{"id":"106","span":{"begin":43,"end":59},"obj":"Species"},{"id":"107","span":{"begin":61,"end":79},"obj":"Species"},{"id":"108","span":{"begin":245,"end":250},"obj":"Chemical"},{"id":"109","span":{"begin":329,"end":349},"obj":"Disease"},{"id":"110","span":{"begin":535,"end":549},"obj":"Disease"},{"id":"111","span":{"begin":625,"end":633},"obj":"Disease"},{"id":"112","span":{"begin":710,"end":719},"obj":"Disease"},{"id":"113","span":{"begin":912,"end":921},"obj":"Disease"},{"id":"114","span":{"begin":1094,"end":1111},"obj":"Disease"},{"id":"115","span":{"begin":695,"end":697},"obj":"CellLine"}],"attributes":[{"id":"A106","pred":"tao:has_database_id","subj":"106","obj":"Tax:10029"},{"id":"A107","pred":"tao:has_database_id","subj":"107","obj":"Tax:10029"},{"id":"A108","pred":"tao:has_database_id","subj":"108","obj":"MESH:D014867"},{"id":"A109","pred":"tao:has_database_id","subj":"109","obj":"MESH:C000657245"},{"id":"A110","pred":"tao:has_database_id","subj":"110","obj":"MESH:C000657245"},{"id":"A111","pred":"tao:has_database_id","subj":"111","obj":"MESH:D007239"},{"id":"A112","pred":"tao:has_database_id","subj":"112","obj":"MESH:D007239"},{"id":"A113","pred":"tao:has_database_id","subj":"113","obj":"MESH:D007239"},{"id":"A114","pred":"tao:has_database_id","subj":"114","obj":"MESH:D007239"},{"id":"A115","pred":"tao:has_database_id","subj":"115","obj":"CVCL:4582"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"24 five‐ to seven‐week‐old female and male Chinese hamsters (Cricetulus griseus) were obtained via the German pet trade and kept in groups of six animals in enriched, individually ventilated cages. The hamsters had ad libidum access to food and water, and were allowed acclimatization to these conditions for seven days prior to SARS‐CoV‐2 infection. Cage temperatures and relative humidities were recorded daily and ranged from 23 to 24°C and from 50% to 65%, respectively. The animals were randomly distributed into two groups: SARS‐CoV‐2‐infected (n = 12; 1 × 105 plaque‐forming units (pfu) in a volume of 30 µl) and mock‐infected (n = 12; 30 µl cell culture supernatant from uninfected Vero E6 cells). The infection was performed exactly as previously described (Osterrieder et al., 2020). Baseline body weights of all hamsters and body temperatures of at least three hamsters per group were measured before infection and then recorded daily throughout the 14‐day experiment. Moreover, clinical signs of all animals were monitored twice daily throughout the experiment. On days 2, 3, 5 and 14 post‐infection (dpi), three randomly assigned hamsters of each group were euthanized by exsanguination under general anaesthesia as described (Nakamura et al., 2017). For virus titrations and RT‐qPCR and/or histopathological examinations, blood, bucco‐laryngeal swabs and lungs (left and right) were collected."}