| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T241 |
0-80 |
Sentence |
denotes |
Alignments subsampled at 10% 100 times were used to estimate substitution rates. |
| T242 |
81-277 |
Sentence |
denotes |
For the set of subsampled alignments for each gene, a mixed-effect likelihood method was used to estimate nonsynonymous (dN) and synonymous (dS) substitution rates globally and at each codon (29). |
| T243 |
278-500 |
Sentence |
denotes |
Maximum-likelihood phylogenies were constructed for each alignment using the software IQ-TREE (55) under a best-fit model determined with ModelFinder (56) to prime the dN and dS estimates before branch length optimization. |
| T244 |
501-555 |
Sentence |
denotes |
This step serves to expedite the optimization process. |
| T245 |
556-669 |
Sentence |
denotes |
Branch length optimization was done with a MG94 model [which is the only model available for this analysis (29)]. |
| T246 |
670-873 |
Sentence |
denotes |
The proportion of each phylogeny evolving under neutral (or negative) selection was determined from the mixture density across lineages for each site, assuming different dN and dS along each branch (57). |
| T247 |
874-1087 |
Sentence |
denotes |
On the same set of subsampled alignments and phylogenies, a fixed-effects likelihood method was used on internal branches to identify sites under pervasive diversifying selection and to estimate global dN/dS (58). |
| T248 |
1088-1397 |
Sentence |
denotes |
Known biases associated with calculating dN/dS on exponentially growing populations (59) were counterbalanced by subsampling phylogenies, as the typical approach to address this bias, which is to ignore terminal branches, would considerably diminish the power of the analysis to detect any significant result. |
| T249 |
1398-1768 |
Sentence |
denotes |
As P values from the fixed-effect likelihood method are uncorrected, results were not averaged over P values; rather, given that P value calculations are conservative for this analysis (58), sites were considered to be under pervasive diversifying selection if their P value was <0.1 in ≥50% of alignments, which would account for a typical 5% false discovery rate (58). |
