| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T268 |
0-25 |
Sentence |
denotes |
Phase contrast tomography |
| T269 |
26-146 |
Sentence |
denotes |
For Covid-19 lung tissue, the scans were recorded at the GINIX endstation of the PETRA III storage ring (DESY, Hamburg). |
| T270 |
147-349 |
Sentence |
denotes |
The projections were acquired at two different photon energies Eph, 8keV and 13.8keV, using the first and third harmonic of the 5 m P10 undulator and a Si(111) channel-cut monochromator, respectively. |
| T271 |
350-444 |
Sentence |
denotes |
Data are shown here only for 8keV, which gave highest contrast for the unstained lung tissue. |
| T272 |
445-530 |
Sentence |
denotes |
Two tomography configurations were combined to cover a larger range of length scales: |
| T273 |
532-549 |
Sentence |
denotes |
(1–parallel beam) |
| T274 |
550-730 |
Sentence |
denotes |
Recordings with the unfocused quasi-parallel beam illumination and a high resolution microscope detection system, resulting in in a FOV of 1.6 mm sampled at a pixel size of 650 nm. |
| T275 |
732-745 |
Sentence |
denotes |
(2–cone beam) |
| T276 |
746-1032 |
Sentence |
denotes |
Holographic recordings with the divergent and coherence filtered beam emanating from a compound focusing system composed of a Kirkpatrick-Baez (KB) mirrors system and an x-ray waveguide, resulting in a FOV of 0.4 mm sampled at a pixel size of about 167 nm (depending on exact geometry). |
| T277 |
1033-1180 |
Sentence |
denotes |
Both configurations were implemented side-by-side, using the same fully-motorized tomography stage and mounting, as detailed in Frohn et al., 2020. |
| T278 |
1181-1235 |
Sentence |
denotes |
A sketch of both configurations is shown in Figure 1 . |
| T279 |
1236-1335 |
Sentence |
denotes |
First, parallel-beam overview scans were acquired of the entire tissue volume embedded in paraffin. |
| T280 |
1336-1564 |
Sentence |
denotes |
To this end a 3.5 mm biopsy punch was taken from the multi-sample tissue block, and then scanned in a stitching-mode yielding a large overview reconstruction, composed of up to 20 individual tomograms (depending on tissue size). |
| T281 |
1565-1825 |
Sentence |
denotes |
To increase image quality and avoid artifacts related to region-of-interest (ROI) or local tomography, a selected 1 mm punch was then taken from the already scanned larger tissue cylinder, and re-scanned, first in the parallel- and then the cone-beam geometry. |
| T282 |
1826-1912 |
Sentence |
denotes |
Experimental and acquisition parameters used for the data shown are listed in Table 3. |
| T283 |
1913-1921 |
Sentence |
denotes |
Table 3. |
| T284 |
1923-2000 |
Sentence |
denotes |
Data acquisition parameters for x-ray phase-contrast tomography measurements. |
| T285 |
2001-2033 |
Sentence |
denotes |
Cone geometry Parallel geometry |
| T286 |
2034-2072 |
Sentence |
denotes |
FOV 0.4 mm × 0.35 mm 1.6 mm × 1.4 mm |
| T287 |
2073-2099 |
Sentence |
denotes |
Pixel size 167 nm 650 nm |
| T288 |
2100-2115 |
Sentence |
denotes |
z 01 125 mm - |
| T289 |
2116-2145 |
Sentence |
denotes |
z 12 4975 mm 10 mm − 100 mm |
| T290 |
2146-2182 |
Sentence |
denotes |
Regime holographic direct contrast |
| T291 |
2183-2215 |
Sentence |
denotes |
Rotation start-stop continuous |
| T292 |
2216-2241 |
Sentence |
denotes |
Exposure 2 s 0.035 s |
| T293 |
2242-2278 |
Sentence |
denotes |
Total exposure ≃ 63 m i n ≃ 75 s |
| T294 |
2279-2420 |
Sentence |
denotes |
Volumetric flowrate 1.16 × 10 4 μ m / 3 s 3.75 × 10 7 μ m / 3 s The configuration for (1–parallel beam) is depicted in Figure 1c. |
| T295 |
2421-2691 |
Sentence |
denotes |
The high-resolution microscope detection system (Optique Peter, France) was based on a 50μm thick LuAG:Ce scintillator imaged with a 10× magnifying microscope objective onto a sCMOS sensor (pco.edge 5.5, PCO, Germany), resulting in an effective pixel size of 0.65μm. |
| T296 |
2692-2852 |
Sentence |
denotes |
The high photon flux density allowed for image acquisition with continuous motor movement, short acquisition time of 35 ms per frame, and a framerate of 20 fps. |
| T297 |
2853-2982 |
Sentence |
denotes |
Single-distance tomogram recordings with about 1500 projections, and flat images before and after the scan, took less than 2 min. |
| T298 |
2983-3180 |
Sentence |
denotes |
For these scans, the focusing optics (KB-mirrors and waveguide) as well as the fastshutter (Cedrat technologies) were moved out of the beam, and beam size was adjusted by the upstream slit systems. |
| T299 |
3181-3271 |
Sentence |
denotes |
To avoid detector saturation, the beam was attenuated by 4× single crystal silicon wafers. |
| T300 |
3272-3333 |
Sentence |
denotes |
The configuration for (2–cone beam) is depicted in Figure 1d: |
| T301 |
3334-3389 |
Sentence |
denotes |
The beam was focused by the KB-mirrors to about 300 nm. |
| T302 |
3390-3845 |
Sentence |
denotes |
To further reduce the secondary source size, to increase coherence, and to achieve a smooth wavefront for holographic illumination, an x-ray waveguide formed by 1 mm long lithographic channels in silicon with a cross-section of about 100 nm was positioned in the focal plane of the KB-mirrors, resulting in an exit flux of 1–4⋅109 photon/s (depending on alignment and storage ring), as measured with the single photon counting detector (Pilatus, Dectris). |
| T303 |
3846-3982 |
Sentence |
denotes |
The sample was positioned at variable (defocus) distances behind the focus (waveguide exit), typically z01=125mm for the first distance. |
| T304 |
3983-4184 |
Sentence |
denotes |
The geometrically magnified holograms were recorded by a fibre-coupled sCMOS sensor (Zyla HF 5.5 detector, Andor Technologies) with a customized 15μm thick Gadox scintillator, and 6.5μm pixel size. |
| T305 |
4185-4364 |
Sentence |
denotes |
The detector position at about z02=5100mm behind the focus resulted in a magnification of about M=41 (for the first defocus distance), and hence an effective pixel size of 167 nm. |
| T306 |
4365-4448 |
Sentence |
denotes |
1442 projections were recorded, with a typical exposure time of 2 s per projection. |
