PMC:7464876 / 63401-64641 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7464876","sourcedb":"PMC","sourceid":"7464876","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7464876","text":"Abrogation of N146-linked glycosylation inhibits HBV release without affecting L/core interaction. (A) HuH-7 cells were cotransfected with the envelope-defective HBV replicon (HBV.Envminus) complemented with expression constructs encoding the wt envelope (Env) or mutant Env.N146D proteins. Three days post-transfection, cellular lysates and supernatants were subjected to L- and core-specific WB analysis and to the virion production assay (n = 3, ± SD). (B) HuH-7 cells were transfected with HBV.Envminus together with control plasmids (Con), the wt envelope (Env) or mutant Env.N146D constructs. To verify equal protein synthesis, lysates were examined with anti-L (K1350) and anti-core (K46) antibodies. For CO-IP analysis, lysates prepared with CHAPS were reacted with anti-core antibodies (K45) followed by L-specific (MA18/7) WB. (C) HuH-7 cells, cotransfected with HBV.Envminus and the mutant Env.N146D expression construct, were processed for immunofluorescence (IF) and stained with anti-L (57761, L, green) and anti-core (B0586, C, red) antibodies. An overlaid image is displayed in the Merge panel, with an outlined area shown at larger magnification. For QCA, PCC values were calculated and represent the mean ± SD. Bar, 10 µM.","tracks":[]}