PMC:7464876 / 36965-38424
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7464876","sourcedb":"PMC","sourceid":"7464876","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7464876","text":"Since ERGIC-53 acts as a high mannose-specific lectin, we next focused on the HBV-specific N-glycans. A number of studies have shown that HBV release requires sugar moieties linked to the N-glycan acceptor N146 of the envelope proteins [11,12,13,14,15,16]. To ascertain the importance of the N146-linked glycans in our experimental settings, we disabled N-glycosylation by creating an asparagine-to-aspartic acid substitution in the HBV envelope expression vector (Env.N146D). This vector was then used for trans-complementation of the envelope-defective replicon HBV.Envminus, followed by WB and VP assays. Consistent with the literature [11,12,13,14,15], the Env.N146D mutant was exclusively synthesized in the nonglycosylated p39 form and, importantly, inhibited the HBV virion release (Figure 6A). Previous studies have attributed the virion release defect of Env.N146D to possible structural alterations of L that might prevent its interaction with the viral capsid [13]. To address this point, we performed CO-IP and CO-IF experiments of HBV-replicating cells expressing either the wt Env or mutant Env.N146D proteins. Neither assay rendered any evidence for a disturbed L/core interaction, as core efficiently coprecipitated Env.N146D (Figure 6B) and thoroughly colocalized with L.N146D in the characteristic CS structure (Figure 6C). We thus infer that the N146-linked N-glycan plays a pivotal role in HBV egress apart from envelope/core interactions.","tracks":[{"project":"2_test","denotations":[{"id":"32806600-29604477-20296224","span":{"begin":237,"end":239},"obj":"29604477"},{"id":"32806600-20881037-20296225","span":{"begin":240,"end":242},"obj":"20881037"},{"id":"32806600-24899172-20296226","span":{"begin":243,"end":245},"obj":"24899172"},{"id":"32806600-30443247-20296227","span":{"begin":249,"end":251},"obj":"30443247"},{"id":"32806600-29604477-20296228","span":{"begin":640,"end":642},"obj":"29604477"},{"id":"32806600-20881037-20296229","span":{"begin":643,"end":645},"obj":"20881037"},{"id":"32806600-24899172-20296230","span":{"begin":646,"end":648},"obj":"24899172"},{"id":"32806600-30443247-20296231","span":{"begin":652,"end":654},"obj":"30443247"},{"id":"32806600-24899172-20296232","span":{"begin":972,"end":974},"obj":"24899172"}],"attributes":[{"subj":"32806600-29604477-20296224","pred":"source","obj":"2_test"},{"subj":"32806600-20881037-20296225","pred":"source","obj":"2_test"},{"subj":"32806600-24899172-20296226","pred":"source","obj":"2_test"},{"subj":"32806600-30443247-20296227","pred":"source","obj":"2_test"},{"subj":"32806600-29604477-20296228","pred":"source","obj":"2_test"},{"subj":"32806600-20881037-20296229","pred":"source","obj":"2_test"},{"subj":"32806600-24899172-20296230","pred":"source","obj":"2_test"},{"subj":"32806600-30443247-20296231","pred":"source","obj":"2_test"},{"subj":"32806600-24899172-20296232","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93e1ec","default":true}]}]}}