PMC:7464876 / 14462-16075 JSONTXT

{"project":"2_test","denotations":[{"id":"32806600-21129143-20296201","span":{"begin":129,"end":131},"obj":"21129143"},{"id":"32806600-10792995-20296202","span":{"begin":856,"end":858},"obj":"10792995"}],"text":"Polyclonal antisera against recombinant native (K45) or denatured (K46) HBV core particles were raised in rabbits, as described [35]. In addition, commercially available polyclonal rabbit (B0586; Dako, Carpinteria, CA, USA) or monoclonal mouse (3HB17; HyTest, Turku, Finland) antisera against the core antigen or capsid particle, respectively, were used. For immunodetection of the HBV L protein, a rabbit antibody raised against a recombinant peptide encoding aa 1–42 of L fused to glutathione S-transferase (K1350; Eurogentec, Liège, Belgium) or the mouse antibodies HepB preS1 (sc-57761; Santa Cruz, CA, USA) and MA18/7 (D. Glebe, University of Giessen, Giessen, Germany), recognizing L-specific epitopes, were employed. For immunoprecipitation (IP) of all three HBV envelope proteins, a rabbit polyclonal antiserum (K38) against the S domain was used [33]. Antibodies obtained from Covance (Covance, HISS Diagnostics, Freiburg im Breisgau, Germany) were mouse anti-HA (16B12) and anti-Myc (9E10). The mouse anti-EAP20 (VPS25: sc-271648) antibody was purchased from Santa Cruz (Santa Cruz, CA, USA), while the mouse anti-ERGIC-53 (OTI1A8) antibody was from Enzo (Enzo Life Sciences, Farmingdale, NY, USA). Antibodies obtained from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA) were rabbit anti-ERGIC-53 (E1031), rabbit anti-Sec24A (HPA056825), anti-β-actin (AC15) and anti-α-tubulin (B-5-1-2). Peroxidase-labeled secondary antibodies were obtained from Dianova (Hamburg, Germany), and fluorophore-labeled antibodies were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific, Waltham, MA, USA)."}