PMC:7443692 / 9723-10229 JSONTXT

{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T39","span":{"begin":447,"end":450},"obj":"Chemical"}],"attributes":[{"id":"A39","pred":"chebi_id","subj":"T39","obj":"http://purl.obolibrary.org/obo/CHEBI_8984"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-PD-NCBITaxon","denotations":[{"id":"T42","span":{"begin":44,"end":54},"obj":"Species"},{"id":"T43","span":{"begin":153,"end":158},"obj":"Species"}],"attributes":[{"id":"A42","pred":"ncbi_taxonomy_id","subj":"T42","obj":"NCBItxid:2697049"},{"id":"A43","pred":"ncbi_taxonomy_id","subj":"T43","obj":"NCBItxid:9606"}],"namespaces":[{"prefix":"NCBItxid","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-sentences","denotations":[{"id":"T50","span":{"begin":0,"end":506},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-PD-MONDO","denotations":[{"id":"T31","span":{"begin":44,"end":54},"obj":"Disease"}],"attributes":[{"id":"A31","pred":"mondo_id","subj":"T31","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-UniProt","denotations":[{"id":"T1359","span":{"begin":159,"end":163},"obj":"Protein"}],"attributes":[{"id":"A1359","pred":"uniprot_id","subj":"T1359","obj":"https://www.uniprot.org/uniprot/Q9UFZ6"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-PD-IDO","denotations":[{"id":"T45","span":{"begin":325,"end":330},"obj":"http://purl.obolibrary.org/obo/CL_0000000"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-PD-FMA","denotations":[{"id":"T47","span":{"begin":325,"end":330},"obj":"Body_part"}],"attributes":[{"id":"A47","pred":"fma_id","subj":"T47","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-PD-GO-BP-0","denotations":[{"id":"T32","span":{"begin":93,"end":106},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T33","span":{"begin":239,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}

{"project":"LitCovid-sample-GO-BP","denotations":[{"id":"T30","span":{"begin":93,"end":106},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T31","span":{"begin":239,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0070085"}],"text":"A trimer-stabilized, soluble variant of the SARS-CoV-2 S that contains 22 canonical N-linked glycosylation sequons per protomer and a soluble version of human ACE2 that contains six, lacking the most C-terminal seventh, canonical N-linked glycosylation sequons (Figure 1 A) were purified from the media of transfected HEK293 cells, and the quaternary structure confirmed by negative EM staining for the S trimer (Figure 1B) and purity examined by SDS-PAGE Coomassie G-250 stained gels for both (Figure 1C)."}