> top > docs > PMC:7443692 > spans > 48615-50316 > annotations

PMC:7443692 / 48615-50316 JSONTXT

Annnotations TAB JSON ListView MergeView

LitCovid-sample-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T261 44-51 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T262 194-201 Chemical denotes proline http://purl.obolibrary.org/obo/CHEBI_17203|http://purl.obolibrary.org/obo/CHEBI_26271
T264 558-565 Chemical denotes Protein http://purl.obolibrary.org/obo/CHEBI_16541
T265 611-614 Chemical denotes NTA http://purl.obolibrary.org/obo/CHEBI_39054|http://purl.obolibrary.org/obo/CHEBI_44557
T267 674-681 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T268 1199-1202 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T269 1535-1538 Chemical denotes CO2 http://purl.obolibrary.org/obo/CHEBI_16526
T270 1598-1601 Chemical denotes NTA http://purl.obolibrary.org/obo/CHEBI_39054|http://purl.obolibrary.org/obo/CHEBI_44557

LitCovid-sample-PD-NCBITaxon

Id Subject Object Predicate Lexical cue ncbi_taxonomy_id
T135 55-69 Species denotes synthetic gene NCBItxid:2005392
T136 98-108 Species denotes SARS-CoV-2 NCBItxid:2697049
T137 350-367 Species denotes expression vector NCBItxid:81076
T138 1101-1107 Species denotes Fisher NCBItxid:76720
T139 1153-1159 Species denotes camera NCBItxid:274080
T140 1221-1226 Species denotes human NCBItxid:9606

LitCovid-sample-sentences

Id Subject Object Predicate Lexical cue
T332 0-427 Sentence denotes To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD).
T333 428-557 Sentence denotes The expression construct was transiently transfected in HEK293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA).
T334 558-802 Sentence denotes Protein was purified from cell supernatants using Ni-NTA resin (QIAGEN, Germany), the eluted fractions containing S protein were pooled, concentrated, and further purified by gel filtration chromatography on a Superose 6 column (GE Healthcare).
T335 803-900 Sentence denotes Negative stain electron microscopy (EM) analysis was performed as described (Shaik et al., 2019).
T336 901-1194 Sentence denotes Briefly, analysis was performed at room temperature with a magnification of 52,000x and a defocus value of 1.5 μm following low-dose procedures, using a Philips Tecnai F20 electron microscope (Thermo Fisher Scientific) equipped with a Gatan US4000 CCD camera and operated at voltage of 200 kV.
T337 1195-1340 Sentence denotes The DNA fragment encoding human ACE2 (1-615) with a 6xHis tag at C terminus was synthesized by Genscript and cloned to the vector pCMV-IRES-puro.
T338 1341-1422 Sentence denotes The expression construct was transfected in HEK293F cells using polyethylenimine.
T339 1423-1499 Sentence denotes The medium was discarded and replaced with FreeStyle 293 medium after 6-8 h.
T340 1500-1625 Sentence denotes After incubation in 37°C with 5.5% CO2 for 5 days, the supernatant was collected and loaded to Ni-NTA resin for purification.
T341 1626-1701 Sentence denotes The elution was concentrated and further purified by a Superdex 200 column.

LitCovid-sample-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T84 98-108 Disease denotes SARS-CoV-2 http://purl.obolibrary.org/obo/MONDO_0100096

LitCovid-sample-UniProt

Id Subject Object Predicate Lexical cue uniprot_id
T3545 38-51 Protein denotes Spike protein https://www.uniprot.org/uniprot/P31340
T3546 124-129 Protein denotes furin https://www.uniprot.org/uniprot/Q9UCZ5|https://www.uniprot.org/uniprot/Q6LBS3|https://www.uniprot.org/uniprot/Q6GTN6|https://www.uniprot.org/uniprot/Q28193|https://www.uniprot.org/uniprot/Q14336|https://www.uniprot.org/uniprot/P23377|https://www.uniprot.org/uniprot/P23188|https://www.uniprot.org/uniprot/P09958
T3554 672-681 Protein denotes S protein https://www.uniprot.org/uniprot/Q9UIP0|https://www.uniprot.org/uniprot/Q9UIN9|https://www.uniprot.org/uniprot/Q9UIN8|https://www.uniprot.org/uniprot/Q9UIN7|https://www.uniprot.org/uniprot/Q9UIN6|https://www.uniprot.org/uniprot/Q9UBH8|https://www.uniprot.org/uniprot/Q9NRH8|https://www.uniprot.org/uniprot/Q9NRH7|https://www.uniprot.org/uniprot/Q9NRH6|https://www.uniprot.org/uniprot/Q9NRH5|https://www.uniprot.org/uniprot/Q9NRH4|https://www.uniprot.org/uniprot/Q9NPG5|https://www.uniprot.org/uniprot/Q9NPE0|https://www.uniprot.org/uniprot/Q9NP52|https://www.uniprot.org/uniprot/Q95IF9|https://www.uniprot.org/uniprot/Q8N5P3|https://www.uniprot.org/uniprot/Q8IZU6|https://www.uniprot.org/uniprot/Q8IZU5|https://www.uniprot.org/uniprot/Q8IZU4|https://www.uniprot.org/uniprot/Q86Z04|https://www.uniprot.org/uniprot/Q7YR44|https://www.uniprot.org/uniprot/Q7LA71|https://www.uniprot.org/uniprot/Q7LA70|https://www.uniprot.org/uniprot/Q5STD2|https://www.uniprot.org/uniprot/Q5SQ85|https://www.uniprot.org/uniprot/Q1XI16|https://www.uniprot.org/uniprot/Q1XI12|https://www.uniprot.org/uniprot/Q15517|https://www.uniprot.org/uniprot/O43509|https://www.uniprot.org/uniprot/O19084|https://www.uniprot.org/uniprot/B0UYZ7|https://www.uniprot.org/uniprot/B0S7V2|https://www.uniprot.org/uniprot/A5A6L9
T3587 1227-1231 Protein denotes ACE2 https://www.uniprot.org/uniprot/Q9UFZ6

LitCovid-sample-PD-IDO

Id Subject Object Predicate Lexical cue
T150 139-143 http://purl.obolibrary.org/obo/BFO_0000029 denotes site
T151 492-497 http://purl.obolibrary.org/obo/CL_0000000 denotes cells
T152 584-588 http://purl.obolibrary.org/obo/CL_0000000 denotes cell
T153 1393-1398 http://purl.obolibrary.org/obo/CL_0000000 denotes cells

LitCovid-sample-PD-FMA

Id Subject Object Predicate Lexical cue fma_id
T148 44-51 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T149 65-69 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T150 194-201 Body_part denotes proline http://purl.org/sig/ont/fma/fma82761
T151 492-497 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T152 558-565 Body_part denotes Protein http://purl.org/sig/ont/fma/fma67257
T153 584-588 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T154 674-681 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T155 1199-1202 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T156 1393-1398 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646

2_test

Id Subject Object Predicate Lexical cue
32841605-30542158-19659550 894-898 30542158 denotes 2019