PMC:7443692 / 48615-49042 JSONTXT

{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T261","span":{"begin":44,"end":51},"obj":"Chemical"},{"id":"T262","span":{"begin":194,"end":201},"obj":"Chemical"}],"attributes":[{"id":"A261","pred":"chebi_id","subj":"T261","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A262","pred":"chebi_id","subj":"T262","obj":"http://purl.obolibrary.org/obo/CHEBI_17203"},{"id":"A263","pred":"chebi_id","subj":"T262","obj":"http://purl.obolibrary.org/obo/CHEBI_26271"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}

{"project":"LitCovid-sample-PD-NCBITaxon","denotations":[{"id":"T135","span":{"begin":55,"end":69},"obj":"Species"},{"id":"T136","span":{"begin":98,"end":108},"obj":"Species"},{"id":"T137","span":{"begin":350,"end":367},"obj":"Species"}],"attributes":[{"id":"A135","pred":"ncbi_taxonomy_id","subj":"T135","obj":"NCBItxid:2005392"},{"id":"A136","pred":"ncbi_taxonomy_id","subj":"T136","obj":"NCBItxid:2697049"},{"id":"A137","pred":"ncbi_taxonomy_id","subj":"T137","obj":"NCBItxid:81076"}],"namespaces":[{"prefix":"NCBItxid","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}

{"project":"LitCovid-sample-sentences","denotations":[{"id":"T332","span":{"begin":0,"end":427},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}

{"project":"LitCovid-sample-PD-MONDO","denotations":[{"id":"T84","span":{"begin":98,"end":108},"obj":"Disease"}],"attributes":[{"id":"A84","pred":"mondo_id","subj":"T84","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}

{"project":"LitCovid-sample-UniProt","denotations":[{"id":"T3545","span":{"begin":38,"end":51},"obj":"Protein"},{"id":"T3546","span":{"begin":124,"end":129},"obj":"Protein"}],"attributes":[{"id":"A3545","pred":"uniprot_id","subj":"T3545","obj":"https://www.uniprot.org/uniprot/P31340"},{"id":"A3546","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/Q9UCZ5"},{"id":"A3547","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/Q6LBS3"},{"id":"A3548","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/Q6GTN6"},{"id":"A3549","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/Q28193"},{"id":"A3550","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/Q14336"},{"id":"A3551","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/P23377"},{"id":"A3552","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/P23188"},{"id":"A3553","pred":"uniprot_id","subj":"T3546","obj":"https://www.uniprot.org/uniprot/P09958"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}

{"project":"LitCovid-sample-PD-IDO","denotations":[{"id":"T150","span":{"begin":139,"end":143},"obj":"http://purl.obolibrary.org/obo/BFO_0000029"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}

{"project":"LitCovid-sample-PD-FMA","denotations":[{"id":"T148","span":{"begin":44,"end":51},"obj":"Body_part"},{"id":"T149","span":{"begin":65,"end":69},"obj":"Body_part"},{"id":"T150","span":{"begin":194,"end":201},"obj":"Body_part"}],"attributes":[{"id":"A148","pred":"fma_id","subj":"T148","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A149","pred":"fma_id","subj":"T149","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A150","pred":"fma_id","subj":"T150","obj":"http://purl.org/sig/ont/fma/fma82761"}],"text":"To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD)."}