PMC:7443692 / 48528-50316
Annnotations
LitCovid-sample-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T261 | 131-138 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T262 | 281-288 | Chemical | denotes | proline | http://purl.obolibrary.org/obo/CHEBI_17203|http://purl.obolibrary.org/obo/CHEBI_26271 |
| T264 | 645-652 | Chemical | denotes | Protein | http://purl.obolibrary.org/obo/CHEBI_16541 |
| T265 | 698-701 | Chemical | denotes | NTA | http://purl.obolibrary.org/obo/CHEBI_39054|http://purl.obolibrary.org/obo/CHEBI_44557 |
| T267 | 761-768 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T268 | 1286-1289 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T269 | 1622-1625 | Chemical | denotes | CO2 | http://purl.obolibrary.org/obo/CHEBI_16526 |
| T270 | 1685-1688 | Chemical | denotes | NTA | http://purl.obolibrary.org/obo/CHEBI_39054|http://purl.obolibrary.org/obo/CHEBI_44557 |
LitCovid-sample-PD-NCBITaxon
| Id | Subject | Object | Predicate | Lexical cue | ncbi_taxonomy_id |
|---|---|---|---|---|---|
| T133 | 50-60 | Species | denotes | SARS-CoV-2 | NCBItxid:2697049 |
| T134 | 67-72 | Species | denotes | Human | NCBItxid:9606 |
| T135 | 142-156 | Species | denotes | synthetic gene | NCBItxid:2005392 |
| T136 | 185-195 | Species | denotes | SARS-CoV-2 | NCBItxid:2697049 |
| T137 | 437-454 | Species | denotes | expression vector | NCBItxid:81076 |
| T138 | 1188-1194 | Species | denotes | Fisher | NCBItxid:76720 |
| T139 | 1240-1246 | Species | denotes | camera | NCBItxid:274080 |
| T140 | 1308-1313 | Species | denotes | human | NCBItxid:9606 |
LitCovid-sample-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T331 | 0-86 | Sentence | denotes | Expression, Purification, and Characterization of SARS-CoV-2 S and Human ACE2 Proteins |
| T332 | 87-514 | Sentence | denotes | To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD). |
| T333 | 515-644 | Sentence | denotes | The expression construct was transiently transfected in HEK293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA). |
| T334 | 645-889 | Sentence | denotes | Protein was purified from cell supernatants using Ni-NTA resin (QIAGEN, Germany), the eluted fractions containing S protein were pooled, concentrated, and further purified by gel filtration chromatography on a Superose 6 column (GE Healthcare). |
| T335 | 890-987 | Sentence | denotes | Negative stain electron microscopy (EM) analysis was performed as described (Shaik et al., 2019). |
| T336 | 988-1281 | Sentence | denotes | Briefly, analysis was performed at room temperature with a magnification of 52,000x and a defocus value of 1.5 μm following low-dose procedures, using a Philips Tecnai F20 electron microscope (Thermo Fisher Scientific) equipped with a Gatan US4000 CCD camera and operated at voltage of 200 kV. |
| T337 | 1282-1427 | Sentence | denotes | The DNA fragment encoding human ACE2 (1-615) with a 6xHis tag at C terminus was synthesized by Genscript and cloned to the vector pCMV-IRES-puro. |
| T338 | 1428-1509 | Sentence | denotes | The expression construct was transfected in HEK293F cells using polyethylenimine. |
| T339 | 1510-1586 | Sentence | denotes | The medium was discarded and replaced with FreeStyle 293 medium after 6-8 h. |
| T340 | 1587-1712 | Sentence | denotes | After incubation in 37°C with 5.5% CO2 for 5 days, the supernatant was collected and loaded to Ni-NTA resin for purification. |
| T341 | 1713-1788 | Sentence | denotes | The elution was concentrated and further purified by a Superdex 200 column. |
LitCovid-sample-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T83 | 50-60 | Disease | denotes | SARS-CoV-2 | http://purl.obolibrary.org/obo/MONDO_0100096 |
| T84 | 185-195 | Disease | denotes | SARS-CoV-2 | http://purl.obolibrary.org/obo/MONDO_0100096 |
LitCovid-sample-UniProt
| Id | Subject | Object | Predicate | Lexical cue | uniprot_id |
|---|---|---|---|---|---|
| T3544 | 73-77 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T3545 | 125-138 | Protein | denotes | Spike protein | https://www.uniprot.org/uniprot/P31340 |
| T3546 | 211-216 | Protein | denotes | furin | https://www.uniprot.org/uniprot/Q9UCZ5|https://www.uniprot.org/uniprot/Q6LBS3|https://www.uniprot.org/uniprot/Q6GTN6|https://www.uniprot.org/uniprot/Q28193|https://www.uniprot.org/uniprot/Q14336|https://www.uniprot.org/uniprot/P23377|https://www.uniprot.org/uniprot/P23188|https://www.uniprot.org/uniprot/P09958 |
| T3554 | 759-768 | Protein | denotes | S protein | https://www.uniprot.org/uniprot/Q9UIP0|https://www.uniprot.org/uniprot/Q9UIN9|https://www.uniprot.org/uniprot/Q9UIN8|https://www.uniprot.org/uniprot/Q9UIN7|https://www.uniprot.org/uniprot/Q9UIN6|https://www.uniprot.org/uniprot/Q9UBH8|https://www.uniprot.org/uniprot/Q9NRH8|https://www.uniprot.org/uniprot/Q9NRH7|https://www.uniprot.org/uniprot/Q9NRH6|https://www.uniprot.org/uniprot/Q9NRH5|https://www.uniprot.org/uniprot/Q9NRH4|https://www.uniprot.org/uniprot/Q9NPG5|https://www.uniprot.org/uniprot/Q9NPE0|https://www.uniprot.org/uniprot/Q9NP52|https://www.uniprot.org/uniprot/Q95IF9|https://www.uniprot.org/uniprot/Q8N5P3|https://www.uniprot.org/uniprot/Q8IZU6|https://www.uniprot.org/uniprot/Q8IZU5|https://www.uniprot.org/uniprot/Q8IZU4|https://www.uniprot.org/uniprot/Q86Z04|https://www.uniprot.org/uniprot/Q7YR44|https://www.uniprot.org/uniprot/Q7LA71|https://www.uniprot.org/uniprot/Q7LA70|https://www.uniprot.org/uniprot/Q5STD2|https://www.uniprot.org/uniprot/Q5SQ85|https://www.uniprot.org/uniprot/Q1XI16|https://www.uniprot.org/uniprot/Q1XI12|https://www.uniprot.org/uniprot/Q15517|https://www.uniprot.org/uniprot/O43509|https://www.uniprot.org/uniprot/O19084|https://www.uniprot.org/uniprot/B0UYZ7|https://www.uniprot.org/uniprot/B0S7V2|https://www.uniprot.org/uniprot/A5A6L9 |
| T3587 | 1314-1318 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
LitCovid-sample-PD-IDO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T150 | 226-230 | http://purl.obolibrary.org/obo/BFO_0000029 | denotes | site |
| T151 | 579-584 | http://purl.obolibrary.org/obo/CL_0000000 | denotes | cells |
| T152 | 671-675 | http://purl.obolibrary.org/obo/CL_0000000 | denotes | cell |
| T153 | 1480-1485 | http://purl.obolibrary.org/obo/CL_0000000 | denotes | cells |
LitCovid-sample-PD-FMA
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T147 | 78-86 | Body_part | denotes | Proteins | http://purl.org/sig/ont/fma/fma67257 |
| T148 | 131-138 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T149 | 152-156 | Body_part | denotes | gene | http://purl.org/sig/ont/fma/fma74402 |
| T150 | 281-288 | Body_part | denotes | proline | http://purl.org/sig/ont/fma/fma82761 |
| T151 | 579-584 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T152 | 645-652 | Body_part | denotes | Protein | http://purl.org/sig/ont/fma/fma67257 |
| T153 | 671-675 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T154 | 761-768 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T155 | 1286-1289 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T156 | 1480-1485 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
2_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 32841605-30542158-19659550 | 981-985 | 30542158 | denotes | 2019 |