PMC:7443692 / 4430-6426
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7443692","sourcedb":"PMC","sourceid":"7443692","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7443692","text":"The SARS-CoV-2 Spike glycoprotein consists of two subunits, a receptor binding subunit (S1) and a membrane fusion subunit (S2) (Lu et al., 2020; Zhou et al., 2020). The Spike glycoprotein assembles into stable homotrimers that together possess 66 canonical sequons for N-linked glycosylation (N-X-S/T, where X is any amino acid except P) as well as a number of potential O-linked glycosylation sites (Watanabe et al., 2020a; Watanabe et al., 2020b). Interestingly, coronaviruses virions bud into the lumen of the endoplasmic reticulum-Golgi intermediate compartment, ERGIC, raising unanswered questions regarding the precise mechanisms by which viral surface glycoproteins are processed as they traverse the secretory pathway (Stertz et al., 2007; Ujike and Taguchi, 2015). Although this and similar studies (Shajahan et al., 2020; Watanabe et al., 2020a) analyze recombinant proteins, a previous study on SARS-CoV-1 suggested that glycosylation of the Spike can be impacted by this intracellular budding, and this remains to be investigated in SARS-CoV-2 (Ritchie et al., 2010). Nonetheless, it has been proposed that this virus, and others, acquires a glycan coat sufficient and similar enough to endogenous host protein glycosylation that it serves as a glycan shield, facilitating immune evasion by masking non-self viral peptides with self-glycans (Stertz et al., 2007; Ujike and Taguchi, 2015; Watanabe et al., 2020b; Watanabe et al., 2019). In parallel with their potential masking functions, glycan-dependent epitopes can elicit specific, even neutralizing, antibody responses, as has been described for HIV-1 (Duan et al., 2018; Escolano et al., 2019; Pinto et al., 2020; Seabright et al., 2020; Watanabe et al., 2019; Yu et al., 2018; https://www.biorxiv.org/content/10.1101/2020.06.30.178897v1). Thus, understanding the glycosylation of the viral Spike trimer is fundamental for the development of efficacious vaccines, neutralizing antibodies, and therapeutic inhibitors of infection.","tracks":[{"project":"LitCovid-sample-MedDRA","denotations":[{"id":"T5","span":{"begin":1029,"end":1041},"obj":"http://purl.bioontology.org/ontology/MEDDRA/10022891"},{"id":"T6","span":{"begin":1552,"end":1574},"obj":"http://purl.bioontology.org/ontology/MEDDRA/10022891"},{"id":"T7","span":{"begin":1931,"end":1954},"obj":"http://purl.bioontology.org/ontology/MEDDRA/10022891"}],"attributes":[{"id":"A5","pred":"meddra_id","subj":"T5","obj":"http://purl.bioontology.org/ontology/MEDDRA/10062026"},{"id":"A6","pred":"meddra_id","subj":"T6","obj":"http://purl.bioontology.org/ontology/MEDDRA/10058063"},{"id":"A7","pred":"meddra_id","subj":"T7","obj":"http://purl.bioontology.org/ontology/MEDDRA/10058063"},{"subj":"T5","pred":"source","obj":"LitCovid-sample-MedDRA"},{"subj":"T6","pred":"source","obj":"LitCovid-sample-MedDRA"},{"subj":"T7","pred":"source","obj":"LitCovid-sample-MedDRA"}]},{"project":"LitCovid-sample-CHEBI","denotations":[{"id":"T19","span":{"begin":21,"end":33},"obj":"Chemical"},{"id":"T20","span":{"begin":175,"end":187},"obj":"Chemical"},{"id":"T21","span":{"begin":317,"end":327},"obj":"Chemical"},{"id":"T22","span":{"begin":659,"end":672},"obj":"Chemical"},{"id":"T23","span":{"begin":876,"end":884},"obj":"Chemical"},{"id":"T24","span":{"begin":1215,"end":1222},"obj":"Chemical"},{"id":"T25","span":{"begin":1326,"end":1334},"obj":"Chemical"},{"id":"T26","span":{"begin":1345,"end":1352},"obj":"Chemical"}],"attributes":[{"id":"A24","pred":"chebi_id","subj":"T24","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A21","pred":"chebi_id","subj":"T21","obj":"http://purl.obolibrary.org/obo/CHEBI_33709"},{"id":"A19","pred":"chebi_id","subj":"T19","obj":"http://purl.obolibrary.org/obo/CHEBI_17089"},{"id":"A22","pred":"chebi_id","subj":"T22","obj":"http://purl.obolibrary.org/obo/CHEBI_17089"},{"id":"A26","pred":"chebi_id","subj":"T26","obj":"http://purl.obolibrary.org/obo/CHEBI_18154"},{"id":"A20","pred":"chebi_id","subj":"T20","obj":"http://purl.obolibrary.org/obo/CHEBI_17089"},{"id":"A23","pred":"chebi_id","subj":"T23","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A25","pred":"chebi_id","subj":"T25","obj":"http://purl.obolibrary.org/obo/CHEBI_16670"},{"subj":"T19","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T20","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T21","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T22","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T23","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T24","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T25","pred":"source","obj":"LitCovid-sample-CHEBI"},{"subj":"T26","pred":"source","obj":"LitCovid-sample-CHEBI"}]},{"project":"LitCovid-sample-PD-NCBITaxon","denotations":[{"id":"T26","span":{"begin":4,"end":14},"obj":"Species"},{"id":"T27","span":{"begin":906,"end":914},"obj":"Species"},{"id":"T28","span":{"begin":1045,"end":1055},"obj":"Species"},{"id":"T29","span":{"begin":1612,"end":1617},"obj":"Species"}],"attributes":[{"id":"A26","pred":"ncbi_taxonomy_id","subj":"T26","obj":"NCBItxid:2697049"},{"id":"A27","pred":"ncbi_taxonomy_id","subj":"T27","obj":"NCBItxid:694009"},{"id":"A28","pred":"ncbi_taxonomy_id","subj":"T28","obj":"NCBItxid:2697049"},{"id":"A29","pred":"ncbi_taxonomy_id","subj":"T29","obj":"NCBItxid:11676"},{"subj":"T26","pred":"source","obj":"LitCovid-sample-PD-NCBITaxon"},{"subj":"T27","pred":"source","obj":"LitCovid-sample-PD-NCBITaxon"},{"subj":"T28","pred":"source","obj":"LitCovid-sample-PD-NCBITaxon"},{"subj":"T29","pred":"source","obj":"LitCovid-sample-PD-NCBITaxon"}],"namespaces":[{"prefix":"NCBItxid","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}]},{"project":"LitCovid-sample-sentences","denotations":[{"id":"T27","span":{"begin":0,"end":164},"obj":"Sentence"},{"id":"T28","span":{"begin":165,"end":449},"obj":"Sentence"},{"id":"T29","span":{"begin":450,"end":773},"obj":"Sentence"},{"id":"T30","span":{"begin":774,"end":1079},"obj":"Sentence"},{"id":"T31","span":{"begin":1080,"end":1447},"obj":"Sentence"},{"id":"T32","span":{"begin":1448,"end":1806},"obj":"Sentence"},{"id":"T33","span":{"begin":1807,"end":1996},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T27","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T28","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T29","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T30","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T31","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T32","pred":"source","obj":"LitCovid-sample-sentences"},{"subj":"T33","pred":"source","obj":"LitCovid-sample-sentences"}]},{"project":"LitCovid-sample-PD-MONDO","denotations":[{"id":"T19","span":{"begin":4,"end":14},"obj":"Disease"},{"id":"T20","span":{"begin":906,"end":914},"obj":"Disease"},{"id":"T21","span":{"begin":1045,"end":1055},"obj":"Disease"},{"id":"T22","span":{"begin":19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