PMC:7443692 / 28657-30919
Annnotations
LitCovid-sample-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T174 | 192-199 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T175 | 240-247 | Chemical | denotes | glycans | http://purl.obolibrary.org/obo/CHEBI_18154 |
| T176 | 301-308 | Chemical | denotes | glycans | http://purl.obolibrary.org/obo/CHEBI_18154 |
| T177 | 347-354 | Chemical | denotes | mannose | http://purl.obolibrary.org/obo/CHEBI_37684 |
| T178 | 366-373 | Chemical | denotes | glycans | http://purl.obolibrary.org/obo/CHEBI_18154 |
| T179 | 422-429 | Chemical | denotes | glycans | http://purl.obolibrary.org/obo/CHEBI_18154 |
| T180 | 570-579 | Chemical | denotes | N-glycans | http://purl.obolibrary.org/obo/CHEBI_59520 |
| T181 | 716-719 | Chemical | denotes | Ser | http://purl.obolibrary.org/obo/CHEBI_17115|http://purl.obolibrary.org/obo/CHEBI_29999 |
| T183 | 1215-1218 | Chemical | denotes | ion | http://purl.obolibrary.org/obo/CHEBI_24870 |
| T184 | 1272-1280 | Chemical | denotes | N-glycan | http://purl.obolibrary.org/obo/CHEBI_59520 |
| T185 | 1338-1345 | Chemical | denotes | glycans | http://purl.obolibrary.org/obo/CHEBI_18154 |
| T186 | 1435-1443 | Chemical | denotes | N-glycan | http://purl.obolibrary.org/obo/CHEBI_59520 |
| T187 | 1926-1933 | Chemical | denotes | glycans | http://purl.obolibrary.org/obo/CHEBI_18154 |
LitCovid-sample-PD-NCBITaxon
| Id | Subject | Object | Predicate | Lexical cue | ncbi_taxonomy_id |
|---|---|---|---|---|---|
| T79 | 954-959 | Species | denotes | Human | NCBItxid:9606 |
| T80 | 1577-1582 | Species | denotes | human | NCBItxid:9606 |
| T81 | 2073-2083 | Species | denotes | SARS-CoV-2 | NCBItxid:2697049 |
LitCovid-sample-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T161 | 0-81 | Sentence | denotes | Glycomics-Informed Glycoproteomics Reveals Complex N-linked Glycosylation of ACE2 |
| T162 | 82-200 | Sentence | denotes | We also analyzed ACE2 glycosylation utilizing the same glycomic and glycoproteomic approaches described for S protein. |
| T163 | 201-430 | Sentence | denotes | Glycomic analyses of released N-linked glycans (Figure 5 A; Table S3) revealed that the majority of glycans on ACE2 are complex with limited high-mannose and hybrid glycans, and we were unable to detect sulfated N-linked glycans. |
| T164 | 431-637 | Sentence | denotes | Glycoproteomic analyses revealed that occupancy was high (> 75%) at all six sites, and significant microheterogeneity dominated by complex N-glycans was observed for each site (Figures 5B–5G; Tables S5–S8). |
| T165 | 638-898 | Sentence | denotes | We also observed, consistent with the O-glycomics (Figure S5; Table S4), that Ser 155 and several S/T residues at the C terminus of ACE2 outside of the peptidase domain were O-glycosylated, but stoichiometry was extremely low (less than 2%; Tables S9 and S10). |
| T166 | 899-1019 | Sentence | denotes | Figure 5 Glycomics-Informed Glycoproteomics of Soluble Human ACE2 Reveals High Occupancy, Complex N-linked Glycosylation |
| T167 | 1020-1108 | Sentence | denotes | (A) Glycans released from soluble, purified ACE2 were permethylated and analyzed by MSn. |
| T168 | 1109-1227 | Sentence | denotes | Structures were assigned, grouped by type and structural features, and prevalence was determined based on ion current. |
| T169 | 1228-1286 | Sentence | denotes | The pie chart shows basic division by broad N-glycan type. |
| T170 | 1287-1355 | Sentence | denotes | The bar graph provides additional detail about the glycans detected. |
| T171 | 1356-1529 | Sentence | denotes | The most abundant structure with a unique categorization by glycomics for each N-glycan type in the pie chart, or above each feature category in the bar graph, is indicated. |
| T172 | 1530-1735 | Sentence | denotes | (B–G) Glycopeptides were prepared from soluble human ACE2 by using multiple combinations of proteases, analyzed by LC-MSn, and the resulting data were searched by using several different software packages. |
| T173 | 1736-1795 | Sentence | denotes | All six sites of N-linked glycosylation are presented here. |
| T174 | 1796-1910 | Sentence | denotes | Displayed in the bar graphs are the individual compositions observed graphed in terms of assigned spectral counts. |
| T175 | 1911-2031 | Sentence | denotes | Representative glycans (as determined by glycomics analysis) for several abundant compositions are shown in SNFG format. |
| T176 | 2032-2148 | Sentence | denotes | The pie chart (analogous to Figure 3 for SARS-CoV-2 S) for each site is displayed in the upper corner of each panel. |
| T177 | 2149-2158 | Sentence | denotes | (B) N053. |
| T178 | 2159-2168 | Sentence | denotes | (C) N090. |
| T179 | 2169-2178 | Sentence | denotes | (D) N103. |
| T180 | 2179-2188 | Sentence | denotes | (E) N322. |
| T181 | 2189-2198 | Sentence | denotes | (F) N432. |
| T182 | 2199-2262 | Sentence | denotes | (G) N546, a site that does not exist in three in 10,000 people. |
LitCovid-sample-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T58 | 2073-2083 | Disease | denotes | SARS-CoV-2 | http://purl.obolibrary.org/obo/MONDO_0100096 |
LitCovid-sample-UniProt
| Id | Subject | Object | Predicate | Lexical cue | uniprot_id |
|---|---|---|---|---|---|
| T1994 | 77-81 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T1995 | 99-103 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T1996 | 190-199 | Protein | denotes | S protein | https://www.uniprot.org/uniprot/Q9UIP0|https://www.uniprot.org/uniprot/Q9UIN9|https://www.uniprot.org/uniprot/Q9UIN8|https://www.uniprot.org/uniprot/Q9UIN7|https://www.uniprot.org/uniprot/Q9UIN6|https://www.uniprot.org/uniprot/Q9UBH8|https://www.uniprot.org/uniprot/Q9NRH8|https://www.uniprot.org/uniprot/Q9NRH7|https://www.uniprot.org/uniprot/Q9NRH6|https://www.uniprot.org/uniprot/Q9NRH5|https://www.uniprot.org/uniprot/Q9NRH4|https://www.uniprot.org/uniprot/Q9NPG5|https://www.uniprot.org/uniprot/Q9NPE0|https://www.uniprot.org/uniprot/Q9NP52|https://www.uniprot.org/uniprot/Q95IF9|https://www.uniprot.org/uniprot/Q8N5P3|https://www.uniprot.org/uniprot/Q8IZU6|https://www.uniprot.org/uniprot/Q8IZU5|https://www.uniprot.org/uniprot/Q8IZU4|https://www.uniprot.org/uniprot/Q86Z04|https://www.uniprot.org/uniprot/Q7YR44|https://www.uniprot.org/uniprot/Q7LA71|https://www.uniprot.org/uniprot/Q7LA70|https://www.uniprot.org/uniprot/Q5STD2|https://www.uniprot.org/uniprot/Q5SQ85|https://www.uniprot.org/uniprot/Q1XI16|https://www.uniprot.org/uniprot/Q1XI12|https://www.uniprot.org/uniprot/Q15517|https://www.uniprot.org/uniprot/O43509|https://www.uniprot.org/uniprot/O19084|https://www.uniprot.org/uniprot/B0UYZ7|https://www.uniprot.org/uniprot/B0S7V2|https://www.uniprot.org/uniprot/A5A6L9 |
| T2029 | 312-316 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T2030 | 770-774 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T2031 | 960-964 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T2032 | 1064-1068 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
| T2033 | 1583-1587 | Protein | denotes | ACE2 | https://www.uniprot.org/uniprot/Q9UFZ6 |
LitCovid-sample-PD-IDO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T86 | 507-512 | http://purl.obolibrary.org/obo/BFO_0000029 | denotes | sites |
| T87 | 602-606 | http://purl.obolibrary.org/obo/BFO_0000029 | denotes | site |
| T88 | 1744-1749 | http://purl.obolibrary.org/obo/BFO_0000029 | denotes | sites |
| T89 | 2096-2100 | http://purl.obolibrary.org/obo/BFO_0000029 | denotes | site |
| T90 | 2211-2215 | http://purl.obolibrary.org/obo/BFO_0000029 | denotes | site |
LitCovid-sample-PD-FMA
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T95 | 192-199 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T96 | 347-354 | Body_part | denotes | mannose | http://purl.org/sig/ont/fma/fma82801 |
| T97 | 1536-1549 | Body_part | denotes | Glycopeptides | http://purl.org/sig/ont/fma/fma82784 |
LitCovid-sample-PD-GO-BP-0
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T69 | 60-73 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | Glycosylation |
| T70 | 104-117 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
| T71 | 1006-1019 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | Glycosylation |
| T72 | 1762-1775 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
LitCovid-sample-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T65 | 60-73 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | Glycosylation |
| T66 | 104-117 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
| T67 | 1006-1019 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | Glycosylation |
| T68 | 1762-1775 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
LitCovid-sample-Glycan
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T93 | 347-354 | http://rdf.glyconavi.org/CarTNa/CarTNa218/trivialname | denotes | mannose |
| T94 | 1736-1739 | https://glytoucan.org/Structures/Glycans/G05518TD | denotes | All |