PMC:7432599 / 5065-7146 JSONTXT

{"project":"2_test","denotations":[{"id":"32752280-21460597-52425998","span":{"begin":1600,"end":1602},"obj":"21460597"},{"id":"T51286","span":{"begin":1600,"end":1602},"obj":"21460597"}],"text":"To evaluate the effect of goat milk intake in the development of obesity and its metabolic consequences, we fed mice with a control diet (control), a high fat diet (HF) or a HF diet supplemented with lyophilized milk from goats fed a conventional diet (HFCD), grazing (HFG) or a conventional diet supplemented with Acacia farnesiana pods (HFAF) for 14 weeks. As expected, at the end of the study, mice fed the HF diet showed a significant increase (p \u003c 0.05) in body weight with respect to those fed the control diet. Interestingly, the three groups of mice fed HF diet supplemented with goat milk (HFCD, HFG, and HFAF) presented similar body weight to those fed the control diet throughout the study period (Figure 1A,B). The lower final body weight of mice fed goat milk with respect to those fed the HF was not caused by a reduction in food or energy intake. As observed in Figure 1C, mice fed HFCD, HFG or HFAF had higher food intake than those fed the HF. Since HF diets are more energy-dense than the control diet, energy intake of mice fed either diet containing goat milk was higher than those fed control or HF diets (p \u003c 0.05) (Figure 1D). The increased body weight of HF mice was due to a significant increment in body fat mass (p \u003c 0.05) and reduced lean mass with respect to control (Figure 1E,F). Notably, mice fed goat’s milk presented no significant differences in fat and lean mass with respect to control except the HFCD group that had lower fat mass and higher lean mass than all other groups. Leptin is an adipose-derived hormone that is secreted in proportion to total body fat [13]. As expected, circulating leptin concentration was directly associated with fat mass in all groups (Figure 1G). To evaluate the effect of dietary goat milk in circulating lipids we determined serum triglycerides and cholesterol. As expected, HF mice presented higher serum triglycerides than control mice (p \u003c 0.05). Nevertheless, HFCD, HFG, and HFAF mice presented significantly lower triglycerides levels than HF mice (p \u003c 0.05), similar to those of control mice (Figure 1H)."}