PMC:7432599 / 37025-38148 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7432599","sourcedb":"PMC","sourceid":"7432599","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7432599","text":"Total phenolic content of the diets was determined by the Folin–Ciocalteu colorimetric method described by Singleton et al. [56]. Briefly, experimental diets were diluted in methanol:water (80:20) and extracted according to Delgadillo-Puga et al. [10], Then, 3 mg of diets extracts were diluted in 1 mL of distilled water. Five hundred µL of each stock solution was transferred into a volumetric flask and 3% HCL was added to a final volume of 5 mL. From the resulting solution, an aliquot of 100 µL was mixed with a 2 mL of 2% NaCO3 solution and allowed to stand during two minutes. Then, 100 µL of Folin–Ciocalteu reagent (previously diluted 1:1 v/v with water) were added to the solution and incubated at room temperature for 30 min protected from light. Absorbance was measured at 765 nm in a 3.0 mL UV-Quartz cell (Hach Co. cat. 48228-00, Loveland, CO, USA) using a UV spectrophotometric equipment (Beckman, DU-70, Brea, CA, USA). A calibration curve of gallic acid was prepared to estimate the total polyphenols concentration. Results were expressed as mg of gallic acid equivalents (GAE) per 100 g of diet (Table 1).","tracks":[]}