PMC:7432599 / 35071-36939
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7432599","sourcedb":"PMC","sourceid":"7432599","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7432599","text":"4.2. Experimental Diets\nAnimals were randomly assigned into five groups (n = 6) receiving one of the following isoenergetic experimental diets: (1) Control; (2) High-fat (HF); (3) HF + dry whole milk from goats fed a conventional diet (HFCD); (4) HF + dry whole milk from goats fed on grazing (HFG); (5) HF + dry whole milk from goats fed a conventional diet supplemented with 30% of Acacia farnesiana pods (HFAF). Goat’s milk was collected and lyophilized according to Delgadillo-Puga et al. [10]. Diets were administered in dry from, and their composition was adjusted according to the recommendations of AIN 93 [55] (Table 1). The study had a duration of twelve weeks where all animals had ad libitum access to water and their respective experimental diet. Body weight was measured once a week and, food intake was measured every other day during the study. Body composition, energy expenditure, glucose and insulin tolerance were evaluated as indicated below at week 10, 11 and 12, respectively as indicated below.\nAt the end of the study, mice were deprived of food six hours previous to the euthanasia mice were deprived of food. Euthanasia was performed by exposing mice to a lethal dose of sevoflurane (fluoromethyl-2,2,2-trifluoro-1-(trifluoromethyl)ethyl ether). Total blood volume was drawn from the posterior vena cava using a 1 mL syringe coated with heparin. Serum was separated by centrifugation for 10 min at 1800× g at 4 °C. Then subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), brown adipose tissue (BAT), liver, pancreas, skeletal muscle (soleus and gastrocnemius) were rapidly removed and divided in smalls parts. Some samples were frozen in liquid nitrogen and stored at −80 °C. The rest of the samples were fixed in ice-cold 4% (w/v) paraformaldehyde in phosphate buffer saline (PBS) for histological analyses as described below.","divisions":[{"label":"title","span":{"begin":0,"end":23}},{"label":"p","span":{"begin":24,"end":1018}}],"tracks":[{"project":"2_test","denotations":[{"id":"32752280-8229312-52426036","span":{"begin":615,"end":617},"obj":"8229312"},{"id":"T54057","span":{"begin":615,"end":617},"obj":"8229312"}],"attributes":[{"subj":"32752280-8229312-52426036","pred":"source","obj":"2_test"},{"subj":"T54057","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93ecad","default":true}]}]}}