PMC:7417788 / 63431-63853 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T1411","span":{"begin":69,"end":73},"obj":"CHEBI:37958;CHEBI:37958"},{"id":"T1412","span":{"begin":74,"end":83},"obj":"CHEBI:27899;CHEBI:27899"},{"id":"T1413","span":{"begin":137,"end":140},"obj":"CHEBI:60004;CHEBI:60004"},{"id":"T1414","span":{"begin":325,"end":333},"obj":"CHEBI:10545;CHEBI:10545"}],"text":"Barcoded and combined samples were washed and stained with viability dyes cisplatin-195pt (0.5 μmolL) (Fluidigm, 201064) and vortexed to mix thoroughly for 2 min at room temperature for cell viability, terminated with Maxpar Cell Staining buffer at room temperature (400 rcf.), washed, fixed with 1.6% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 10 min at room temperature on a rotary shaker (500 rpm)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T454","span":{"begin":186,"end":190},"obj":"Body_part"},{"id":"T455","span":{"begin":225,"end":229},"obj":"Body_part"}],"attributes":[{"id":"A454","pred":"fma_id","subj":"T454","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A455","pred":"fma_id","subj":"T455","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Barcoded and combined samples were washed and stained with viability dyes cisplatin-195pt (0.5 μmolL) (Fluidigm, 201064) and vortexed to mix thoroughly for 2 min at room temperature for cell viability, terminated with Maxpar Cell Staining buffer at room temperature (400 rcf.), washed, fixed with 1.6% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 10 min at room temperature on a rotary shaker (500 rpm)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1081","span":{"begin":186,"end":190},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1082","span":{"begin":225,"end":229},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1083","span":{"begin":396,"end":397},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Barcoded and combined samples were washed and stained with viability dyes cisplatin-195pt (0.5 μmolL) (Fluidigm, 201064) and vortexed to mix thoroughly for 2 min at room temperature for cell viability, terminated with Maxpar Cell Staining buffer at room temperature (400 rcf.), washed, fixed with 1.6% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 10 min at room temperature on a rotary shaker (500 rpm)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T105","span":{"begin":69,"end":73},"obj":"Chemical"},{"id":"T85198","span":{"begin":74,"end":83},"obj":"Chemical"},{"id":"T44955","span":{"begin":239,"end":245},"obj":"Chemical"},{"id":"T87833","span":{"begin":320,"end":323},"obj":"Chemical"}],"attributes":[{"id":"A71025","pred":"chebi_id","subj":"T105","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"},{"id":"A73597","pred":"chebi_id","subj":"T85198","obj":"http://purl.obolibrary.org/obo/CHEBI_27899"},{"id":"A94173","pred":"chebi_id","subj":"T44955","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A58675","pred":"chebi_id","subj":"T87833","obj":"http://purl.obolibrary.org/obo/CHEBI_53371"},{"id":"A51579","pred":"chebi_id","subj":"T87833","obj":"http://purl.obolibrary.org/obo/CHEBI_60594"}],"text":"Barcoded and combined samples were washed and stained with viability dyes cisplatin-195pt (0.5 μmolL) (Fluidigm, 201064) and vortexed to mix thoroughly for 2 min at room temperature for cell viability, terminated with Maxpar Cell Staining buffer at room temperature (400 rcf.), washed, fixed with 1.6% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 10 min at room temperature on a rotary shaker (500 rpm)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T351","span":{"begin":0,"end":422},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Barcoded and combined samples were washed and stained with viability dyes cisplatin-195pt (0.5 μmolL) (Fluidigm, 201064) and vortexed to mix thoroughly for 2 min at room temperature for cell viability, terminated with Maxpar Cell Staining buffer at room temperature (400 rcf.), washed, fixed with 1.6% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 10 min at room temperature on a rotary shaker (500 rpm)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1431","span":{"begin":74,"end":83},"obj":"Chemical"},{"id":"1432","span":{"begin":302,"end":318},"obj":"Chemical"},{"id":"1433","span":{"begin":320,"end":323},"obj":"Chemical"},{"id":"1434","span":{"begin":358,"end":361},"obj":"Chemical"}],"attributes":[{"id":"A1431","pred":"tao:has_database_id","subj":"1431","obj":"MESH:D002945"},{"id":"A1432","pred":"tao:has_database_id","subj":"1432","obj":"MESH:C003043"},{"id":"A1433","pred":"tao:has_database_id","subj":"1433","obj":"MESH:C003043"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Barcoded and combined samples were washed and stained with viability dyes cisplatin-195pt (0.5 μmolL) (Fluidigm, 201064) and vortexed to mix thoroughly for 2 min at room temperature for cell viability, terminated with Maxpar Cell Staining buffer at room temperature (400 rcf.), washed, fixed with 1.6% paraformaldehyde (PFA; Electron Microscopy Sciences) in PBS for 10 min at room temperature on a rotary shaker (500 rpm)."}