PMC:7376845 / 9948-11188
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T67 | 39-46 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T68 | 215-222 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T69 | 436-441 | Body_part | denotes | serum | http://purl.org/sig/ont/fma/fma63083 |
| T70 | 679-682 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T4 | 436-441 | Body_part | denotes | serum | http://purl.obolibrary.org/obo/UBERON_0001977 |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 270 | 436-449 | Gene | denotes | serum albumin | Gene:11657 |
| 271 | 26-36 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 272 | 479-485 | Species | denotes | murine | Tax:10090 |
| 273 | 864-894 | Chemical | denotes | 3,3',5,5'-tetramethylbenzidine | MESH:C021758 |
| 274 | 896-899 | Chemical | denotes | TMB | MESH:C021758 |
| 275 | 1010-1024 | Chemical | denotes | sulphuric acid | MESH:C033158 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T110 | 26-34 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T111 | 26-30 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T124 | 177-179 | http://purl.obolibrary.org/obo/CLO_0001302 | denotes | 34 |
| T125 | 195-196 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T126 | 259-260 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T127 | 334-338 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T128 | 663-667 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T129 | 673-678 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T130 | 1080-1081 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T99 | 39-46 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T100 | 155-161 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T101 | 169-175 | Chemical | denotes | NaHCO3 | http://purl.obolibrary.org/obo/CHEBI_32139 |
| T102 | 183-189 | Chemical | denotes | Na2CO3 | http://purl.obolibrary.org/obo/CHEBI_29377 |
| T103 | 215-222 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T104 | 528-534 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T105 | 751-757 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T106 | 1010-1024 | Chemical | denotes | sulphuric acid | http://purl.obolibrary.org/obo/CHEBI_26836 |
| T107 | 1020-1024 | Chemical | denotes | acid | http://purl.obolibrary.org/obo/CHEBI_37527 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T71 | 0-5 | Sentence | denotes | ELISA |
| T72 | 6-116 | Sentence | denotes | Whole ectodomain of SARS-CoV-2 S protein with His Tag (Sino Biological Inc., Beijing, China; catalogue number: |
| T73 | 117-339 | Sentence | denotes | 40589-B08V1) was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and a total of 20 ng of protein was loaded into individual wells of a 96 well plate (Nunc, Roskilde, Denmark) and allowed to coat overnight at 4 °C. |
| T74 | 340-574 | Sentence | denotes | Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour. |
| T75 | 575-791 | Sentence | denotes | Plate were washed four times with PBST before incubation for 1 hour with HRP-conjugated goat anti-mouse IgG (Thermo Fisher Scientific) secondary antibodies diluted in blocking buffer, and washed four times with PBST. |
| T76 | 792-1025 | Sentence | denotes | Visualisation of bound secondary antibodies was done by the addition of 3,3',5,5'-tetramethylbenzidine (TMB) substrate (Thermo Fisher Scientific) for 5 min in the absence of light and the reaction was stopped with 2 M sulphuric acid. |
| T77 | 1026-1240 | Sentence | denotes | Optical density at 450 nm (OD450nm) was determined by a Tecan (Männedorf, Switzerland) Infinite M1000 reader and normalised OD450nm was obtained by subtracting background absorbances determined in BSA coated wells. |