PMC:7376845 / 8883-9946
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T61 | 99-104 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T62 | 346-351 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T63 | 416-421 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T64 | 548-551 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
| T65 | 552-560 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T66 | 922-926 | Body_part | denotes | lens | http://purl.org/sig/ont/fma/fma58241 |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 256 | 478-484 | Species | denotes | murine | Tax:10090 |
| 257 | 197-213 | Chemical | denotes | paraformaldehyde | MESH:C003043 |
| 258 | 270-282 | Chemical | denotes | Triton X-100 | MESH:D017830 |
| 259 | 375-378 | Chemical | denotes | PBS | |
| 260 | 505-520 | Chemical | denotes | Alexa Fluor 488 | |
| 261 | 662-691 | Chemical | denotes | 4′,6-diamidino-2-phenylindole | MESH:C007293 |
| 262 | 693-697 | Chemical | denotes | DAPI | MESH:C007293 |
| 263 | 93-98 | CellLine | denotes | COS-7 | CVCL:0224 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T108 | 93-104 | http://purl.obolibrary.org/obo/CLO_0051656 | denotes | COS-7 cells |
| T109 | 93-104 | http://purl.obolibrary.org/obo/CLO_0051657 | denotes | COS-7 cells |
| T110 | 93-104 | http://purl.obolibrary.org/obo/CLO_0051658 | denotes | COS-7 cells |
| T111 | 93-98 | http://purl.obolibrary.org/obo/CLO_0002597 | denotes | COS-7 |
| T112 | 93-98 | http://purl.obolibrary.org/obo/CLO_0050508 | denotes | COS-7 |
| T113 | 299-301 | http://purl.obolibrary.org/obo/CLO_0009141 | denotes | St |
| T114 | 299-301 | http://purl.obolibrary.org/obo/CLO_0050980 | denotes | St |
| T115 | 310-312 | http://purl.obolibrary.org/obo/CLO_0007815 | denotes | MO |
| T116 | 346-351 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T117 | 416-421 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T118 | 493-495 | http://purl.obolibrary.org/obo/CLO_0053799 | denotes | 45 |
| T119 | 532-536 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T120 | 542-547 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T121 | 908-911 | http://purl.obolibrary.org/obo/CL_0000784 | denotes | iPC |
| T122 | 912-921 | http://purl.obolibrary.org/obo/BFO_0000030 | denotes | objective |
| T123 | 922-926 | http://purl.obolibrary.org/obo/UBERON_0005389 | denotes | lens |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T93 | 270-282 | Chemical | denotes | Triton X-100 | http://purl.obolibrary.org/obo/CHEBI_9750 |
| T94 | 505-520 | Chemical | denotes | Alexa Fluor 488 | http://purl.obolibrary.org/obo/CHEBI_52661 |
| T95 | 511-516 | Chemical | denotes | Fluor | http://purl.obolibrary.org/obo/CHEBI_24061 |
| T96 | 679-691 | Chemical | denotes | phenylindole | http://purl.obolibrary.org/obo/CHEBI_48559 |
| T97 | 693-697 | Chemical | denotes | DAPI | http://purl.obolibrary.org/obo/CHEBI_51231 |
| T98 | 741-745 | Chemical | denotes | Gold | http://purl.obolibrary.org/obo/CHEBI_29287 |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T64 | 0-54 | Sentence | denotes | Transient transfection and immunofluorescence analysis |
| T65 | 55-339 | Sentence | denotes | For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. |
| T66 | 340-415 | Sentence | denotes | Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. |
| T67 | 416-610 | Sentence | denotes | Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). |
| T68 | 611-810 | Sentence | denotes | Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). |
| T69 | 811-989 | Sentence | denotes | Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. |
| T70 | 990-1063 | Sentence | denotes | Each channel was collected separately, with images at 1024 × 1024 pixels. |