PMC:7376845 / 8335-8522 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T59","span":{"begin":39,"end":43},"obj":"Body_part"}],"attributes":[{"id":"A59","pred":"fma_id","subj":"T59","obj":"http://purl.org/sig/ont/fma/fma62100"}],"text":"The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T3","span":{"begin":39,"end":43},"obj":"Body_part"}],"attributes":[{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight."}

{"project":"LitCovid-PubTator","denotations":[{"id":"244","span":{"begin":47,"end":67},"obj":"Chemical"},{"id":"245","span":{"begin":79,"end":87},"obj":"Chemical"},{"id":"246","span":{"begin":89,"end":93},"obj":"Chemical"}],"attributes":[{"id":"A245","pred":"tao:has_database_id","subj":"245","obj":"MESH:D011136"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T103","span":{"begin":4,"end":12},"obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"id":"T104","span":{"begin":172,"end":176},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"}],"text":"The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T91","span":{"begin":47,"end":51},"obj":"Chemical"}],"attributes":[{"id":"A91","pred":"chebi_id","subj":"T91","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"}],"text":"The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight."}

{"project":"LitCovid-sentences","denotations":[{"id":"T61","span":{"begin":0,"end":187},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight."}