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PMC:7376845 / 7522-8881 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T51 6-11 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T52 101-104 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T53 227-232 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T54 337-342 Body_part denotes Cells http://purl.org/sig/ont/fma/fma68646
T55 453-460 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T56 507-511 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T57 555-559 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T58 642-646 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T59 852-856 Body_part denotes milk http://purl.org/sig/ont/fma/fma62100
T60 1116-1124 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T3 852-856 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
237 778-781 Gene denotes Rad Gene:6236
238 1354-1357 Gene denotes Rad Gene:6236
239 1066-1077 Species denotes horseradish Tax:3704
240 298-323 Chemical denotes phosphate buffered saline
241 325-328 Chemical denotes PBS
242 368-392 Chemical denotes 2× Laemmli sample buffer
243 714-717 Chemical denotes SDS MESH:D012967
244 860-880 Chemical denotes Tris-buffered saline
245 892-900 Chemical denotes Tween 20 MESH:D011136
246 902-906 Chemical denotes TBST
247 0-5 CellLine denotes 293FT CVCL:6911

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T93 6-11 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T94 98-100 http://purl.obolibrary.org/obo/CLO_0003797 denotes HP
T95 98-100 http://purl.obolibrary.org/obo/PR_000008725 denotes HP
T96 227-232 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T97 337-342 http://purl.obolibrary.org/obo/GO_0005623 denotes Cells
T98 507-511 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T99 536-540 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T100 555-559 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T101 642-646 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T102 764-772 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane
T103 817-825 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane
T104 985-989 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T105 1011-1019 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane
T106 1064-1065 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T107 1174-1182 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T80 98-100 Chemical denotes HP http://purl.obolibrary.org/obo/CHEBI_74055
T81 101-104 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T82 118-125 Chemical denotes reagent http://purl.obolibrary.org/obo/CHEBI_33893
T83 298-307 Chemical denotes phosphate http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474
T87 386-392 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T88 453-460 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T89 714-717 Chemical denotes SDS http://purl.obolibrary.org/obo/CHEBI_8984
T90 749-763 Chemical denotes nitrocellulose http://purl.obolibrary.org/obo/CHEBI_53325
T91 860-864 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T92 1331-1333 Chemical denotes MP http://purl.obolibrary.org/obo/CHEBI_73612

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T2 1066-1094 http://purl.obolibrary.org/obo/GO_0004601 denotes horseradish peroxidase (HRP)

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T56 0-195 Sentence denotes 293FT cells were seeded onto 6-cm dishes 24 hours before transient transfection using X-tremeGENE HP DNA transfection reagent (Roche, Basel, Switzerland) according to the manufacturer’s protocol.
T57 196-336 Sentence denotes At 24 hours post-transfection, cells were harvested, spun down by centrifugation and washed with cold phosphate buffered saline (PBS) twice.
T58 337-415 Sentence denotes Cells were then resuspended in 2× Laemmli sample buffer, boiled and sonicated.
T59 416-618 Sentence denotes Clarified supernatant containing the protein of interest was obtained by spinning down the cell lysate at 13,000 rpm at 4 °C to remove the cell debris and further analysed by western blot (WB) analysis.
T60 619-812 Sentence denotes Equal amounts of total cell lysates were loaded per lane and resolved using electrophoresis on SDS-PAGE gels and transferred onto nitrocellulose membrane (Bio-Rad, Hercules, CA, United States).
T61 813-1000 Sentence denotes The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight.
T62 1001-1169 Sentence denotes After the membrane was washed with TBST, it was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Thermo Fisher Scientific) at RT for 1 hour.
T63 1170-1359 Sentence denotes The membrane was then washed with TBST again and bound antibodies visualised with enhanced chemiluminescence substrate (Thermo Fisher Scientific) using ChemiDoc MP Imaging System (Bio-Rad).