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PMC:7376845 / 7473-8881 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T51 55-60 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T52 150-153 Body_part denotes DNA http://purl.org/sig/ont/fma/fma74412
T53 276-281 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T54 386-391 Body_part denotes Cells http://purl.org/sig/ont/fma/fma68646
T55 502-509 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T56 556-560 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T57 604-608 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T58 691-695 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T59 901-905 Body_part denotes milk http://purl.org/sig/ont/fma/fma62100
T60 1165-1173 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T3 901-905 Body_part denotes milk http://purl.obolibrary.org/obo/UBERON_0001913

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
237 827-830 Gene denotes Rad Gene:6236
238 1403-1406 Gene denotes Rad Gene:6236
239 1115-1126 Species denotes horseradish Tax:3704
240 347-372 Chemical denotes phosphate buffered saline
241 374-377 Chemical denotes PBS
242 417-441 Chemical denotes 2× Laemmli sample buffer
243 763-766 Chemical denotes SDS MESH:D012967
244 909-929 Chemical denotes Tris-buffered saline
245 941-949 Chemical denotes Tween 20 MESH:D011136
246 951-955 Chemical denotes TBST
247 49-54 CellLine denotes 293FT CVCL:6911

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T93 55-60 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T94 147-149 http://purl.obolibrary.org/obo/CLO_0003797 denotes HP
T95 147-149 http://purl.obolibrary.org/obo/PR_000008725 denotes HP
T96 276-281 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T97 386-391 http://purl.obolibrary.org/obo/GO_0005623 denotes Cells
T98 556-560 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T99 585-589 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T100 604-608 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T101 691-695 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T102 813-821 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane
T103 866-874 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane
T104 1034-1038 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4 °C
T105 1060-1068 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane
T106 1113-1114 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T107 1223-1231 http://purl.obolibrary.org/obo/UBERON_0000158 denotes membrane

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T80 147-149 Chemical denotes HP http://purl.obolibrary.org/obo/CHEBI_74055
T81 150-153 Chemical denotes DNA http://purl.obolibrary.org/obo/CHEBI_16991
T82 167-174 Chemical denotes reagent http://purl.obolibrary.org/obo/CHEBI_33893
T83 347-356 Chemical denotes phosphate http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474
T87 435-441 Chemical denotes buffer http://purl.obolibrary.org/obo/CHEBI_35225
T88 502-509 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T89 763-766 Chemical denotes SDS http://purl.obolibrary.org/obo/CHEBI_8984
T90 798-812 Chemical denotes nitrocellulose http://purl.obolibrary.org/obo/CHEBI_53325
T91 909-913 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T92 1380-1382 Chemical denotes MP http://purl.obolibrary.org/obo/CHEBI_73612

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T2 1115-1143 http://purl.obolibrary.org/obo/GO_0004601 denotes horseradish peroxidase (HRP)

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T55 0-48 Sentence denotes Transient transfection and western blot analysis
T56 49-244 Sentence denotes 293FT cells were seeded onto 6-cm dishes 24 hours before transient transfection using X-tremeGENE HP DNA transfection reagent (Roche, Basel, Switzerland) according to the manufacturer’s protocol.
T57 245-385 Sentence denotes At 24 hours post-transfection, cells were harvested, spun down by centrifugation and washed with cold phosphate buffered saline (PBS) twice.
T58 386-464 Sentence denotes Cells were then resuspended in 2× Laemmli sample buffer, boiled and sonicated.
T59 465-667 Sentence denotes Clarified supernatant containing the protein of interest was obtained by spinning down the cell lysate at 13,000 rpm at 4 °C to remove the cell debris and further analysed by western blot (WB) analysis.
T60 668-861 Sentence denotes Equal amounts of total cell lysates were loaded per lane and resolved using electrophoresis on SDS-PAGE gels and transferred onto nitrocellulose membrane (Bio-Rad, Hercules, CA, United States).
T61 862-1049 Sentence denotes The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight.
T62 1050-1218 Sentence denotes After the membrane was washed with TBST, it was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Thermo Fisher Scientific) at RT for 1 hour.
T63 1219-1408 Sentence denotes The membrane was then washed with TBST again and bound antibodies visualised with enhanced chemiluminescence substrate (Thermo Fisher Scientific) using ChemiDoc MP Imaging System (Bio-Rad).