PMC:7376845 / 5688-15557
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T37 | 9-14 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T38 | 33-38 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T39 | 272-277 | Body_part | denotes | serum | http://purl.org/sig/ont/fma/fma63083 |
| T40 | 405-410 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T41 | 617-622 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T42 | 689-697 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T43 | 787-791 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T44 | 826-833 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T45 | 1119-1126 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T46 | 1208-1215 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T47 | 1243-1250 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T48 | 1309-1313 | Body_part | denotes | gene | http://purl.org/sig/ont/fma/fma74402 |
| T49 | 1464-1471 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T50 | 1521-1528 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T51 | 1840-1845 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T52 | 1935-1938 | Body_part | denotes | DNA | http://purl.org/sig/ont/fma/fma74412 |
| T53 | 2061-2066 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T54 | 2171-2176 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T55 | 2287-2294 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T56 | 2341-2345 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T57 | 2389-2393 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T58 | 2476-2480 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T59 | 2686-2690 | Body_part | denotes | milk | http://purl.org/sig/ont/fma/fma62100 |
| T60 | 2950-2958 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T61 | 3294-3299 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T62 | 3541-3546 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T63 | 3611-3616 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T64 | 3743-3746 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
| T65 | 3747-3755 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T66 | 4117-4121 | Body_part | denotes | lens | http://purl.org/sig/ont/fma/fma58241 |
| T67 | 4299-4306 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T68 | 4475-4482 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T69 | 4696-4701 | Body_part | denotes | serum | http://purl.org/sig/ont/fma/fma63083 |
| T70 | 4939-4942 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
| T71 | 5527-5535 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T72 | 5782-5786 | Body_part | denotes | gene | http://purl.org/sig/ont/fma/fma74402 |
| T73 | 5991-5996 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T74 | 6063-6070 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T75 | 6236-6244 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T76 | 6492-6499 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T77 | 6745-6753 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T78 | 6896-6899 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
| T79 | 7501-7506 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T80 | 7722-7727 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T81 | 7777-7782 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T82 | 7814-7819 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T83 | 7945-7950 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T84 | 8060-8063 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
| T85 | 8064-8072 | Body_part | denotes | antibody | http://purl.org/sig/ont/fma/fma62871 |
| T86 | 8155-8160 | Body_part | denotes | Cells | http://purl.org/sig/ont/fma/fma68646 |
| T87 | 8293-8300 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T88 | 8913-8923 | Body_part | denotes | amino-acid | http://purl.org/sig/ont/fma/fma82739 |
| T89 | 9016-9023 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T90 | 9101-9108 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T91 | 9128-9138 | Body_part | denotes | amino acid | http://purl.org/sig/ont/fma/fma82739 |
| T92 | 9203-9210 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T93 | 9638-9645 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T94 | 9689-9696 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
| T95 | 9701-9711 | Body_part | denotes | amino-acid | http://purl.org/sig/ont/fma/fma82739 |
LitCovid-PD-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T2 | 272-277 | Body_part | denotes | serum | http://purl.obolibrary.org/obo/UBERON_0001977 |
| T3 | 2686-2690 | Body_part | denotes | milk | http://purl.obolibrary.org/obo/UBERON_0001913 |
| T4 | 4696-4701 | Body_part | denotes | serum | http://purl.obolibrary.org/obo/UBERON_0001977 |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 186 | 142-176 | Chemical | denotes | Dulbecco’s Modified Eagle’s Medium | |
| 187 | 178-182 | Chemical | denotes | DMEM | |
| 188 | 333-343 | Chemical | denotes | penicillin | MESH:D010406 |
| 189 | 358-370 | Chemical | denotes | streptomycin | MESH:D013307 |
| 190 | 485-489 | Chemical | denotes | DMEM | |
| 191 | 530-540 | Chemical | denotes | penicillin | MESH:D010406 |
| 192 | 552-564 | Chemical | denotes | streptomycin | MESH:D013307 |
| 193 | 656-659 | Chemical | denotes | CO2 | MESH:D002245 |
| 194 | 27-32 | CellLine | denotes | COS-7 | CVCL:0224 |
| 195 | 399-404 | CellLine | denotes | 293FT | CVCL:6911 |
| 200 | 961-984 | Chemical | denotes | sodium dodecyl sulphate | MESH:D012967 |
| 201 | 985-999 | Chemical | denotes | polyacrylamide | MESH:C016679 |
| 202 | 1021-1024 | Chemical | denotes | SDS | MESH:D012967 |
| 203 | 1104-1118 | Disease | denotes | Coomassie Plus | MESH:D007625 |
| 205 | 1202-1207 | Gene | denotes | spike | Gene:43740568 |
| 216 | 1508-1511 | Gene | denotes | Myc | Gene:4609 |
| 217 | 1519-1528 | Gene | denotes | S-protein | Gene:43740568 |
| 218 | 1605-1608 | Gene | denotes | Myc | Gene:4609 |
| 219 | 1702-1705 | Gene | denotes | Myc | Gene:4609 |
| 220 | 1673-1676 | Gene | denotes | Myc | Gene:4609 |
| 221 | 1241-1250 | Gene | denotes | S-protein | Gene:43740568 |
| 222 | 1230-1240 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 223 | 1572-1582 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 224 | 1745-1750 | Chemical | denotes | HBcAg | |
| 225 | 1713-1722 | Disease | denotes | hepatitis | MESH:D056486 |
| 237 | 2612-2615 | Gene | denotes | Rad | Gene:6236 |
| 238 | 3188-3191 | Gene | denotes | Rad | Gene:6236 |
| 239 | 2900-2911 | Species | denotes | horseradish | Tax:3704 |
| 240 | 2132-2157 | Chemical | denotes | phosphate buffered saline | |
| 241 | 2159-2162 | Chemical | denotes | PBS | |
| 242 | 2202-2226 | Chemical | denotes | 2× Laemmli sample buffer | |
| 243 | 2548-2551 | Chemical | denotes | SDS | MESH:D012967 |
| 244 | 2694-2714 | Chemical | denotes | Tris-buffered saline | |
| 245 | 2726-2734 | Chemical | denotes | Tween 20 | MESH:D011136 |
| 246 | 2736-2740 | Chemical | denotes | TBST | |
| 247 | 1834-1839 | CellLine | denotes | 293FT | CVCL:6911 |
| 256 | 3673-3679 | Species | denotes | murine | Tax:10090 |
| 257 | 3392-3408 | Chemical | denotes | paraformaldehyde | MESH:C003043 |
| 258 | 3465-3477 | Chemical | denotes | Triton X-100 | MESH:D017830 |
| 259 | 3570-3573 | Chemical | denotes | PBS | |
| 260 | 3700-3715 | Chemical | denotes | Alexa Fluor 488 | |
| 261 | 3857-3886 | Chemical | denotes | 4′,6-diamidino-2-phenylindole | MESH:C007293 |
| 262 | 3888-3892 | Chemical | denotes | DAPI | MESH:C007293 |
| 263 | 3288-3293 | CellLine | denotes | COS-7 | CVCL:0224 |
| 270 | 4696-4709 | Gene | denotes | serum albumin | Gene:11657 |
| 271 | 4286-4296 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 272 | 4739-4745 | Species | denotes | murine | Tax:10090 |
| 273 | 5124-5154 | Chemical | denotes | 3,3',5,5'-tetramethylbenzidine | MESH:C021758 |
| 274 | 5156-5159 | Chemical | denotes | TMB | MESH:C021758 |
| 275 | 5270-5284 | Chemical | denotes | sulphuric acid | MESH:C033158 |
| 279 | 5547-5552 | Species | denotes | human | Tax:9606 |
| 280 | 5557-5563 | Chemical | denotes | CR3022 | |
| 281 | 5987-5996 | CellLine | denotes | 293 cells | CVCL:0045 |
| 289 | 6479-6489 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 290 | 6569-6573 | Species | denotes | H7N7 | Tax:119218 |
| 291 | 6890-6895 | Species | denotes | human | Tax:9606 |
| 292 | 6200-6206 | Chemical | denotes | NaHCO3 | MESH:D017693 |
| 293 | 6214-6220 | Chemical | denotes | Na2CO3 | |
| 294 | 6992-6995 | Chemical | denotes | TMB | MESH:C021758 |
| 295 | 7068-7082 | Chemical | denotes | sulphuric acid | MESH:C033158 |
| 297 | 7335-7350 | Disease | denotes | Virus infection | MESH:D001102 |
| 314 | 7526-7536 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 315 | 7538-7548 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 316 | 7556-7561 | Species | denotes | human | Tax:9606 |
| 317 | 7655-7659 | Chemical | denotes | DMEM | |
| 318 | 7752-7760 | Chemical | denotes | methanol | MESH:D000432 |
| 319 | 7761-7768 | Chemical | denotes | acetone | MESH:D000096 |
| 320 | 7907-7918 | Chemical | denotes | Triton ×100 | MESH:D017830 |
| 321 | 7927-7930 | Chemical | denotes | PBS | |
| 322 | 7980-7983 | Chemical | denotes | PBS | |
| 323 | 8017-8032 | Chemical | denotes | Alexa Fluor 488 | |
| 324 | 8124-8128 | Chemical | denotes | DAPI | MESH:C007293 |
| 325 | 8190-8193 | Chemical | denotes | PBS | |
| 326 | 7512-7520 | Disease | denotes | infected | MESH:D007239 |
| 327 | 7629-7638 | Disease | denotes | infection | MESH:D007239 |
| 328 | 7711-7720 | Disease | denotes | infection | MESH:D007239 |
| 329 | 7493-7500 | CellLine | denotes | Vero-E6 | CVCL:0574 |
| 341 | 8325-8333 | Species | denotes | SARS-CoV | Tax:694009 |
| 342 | 8337-8347 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 343 | 8404-8409 | Species | denotes | human | Tax:9606 |
| 344 | 8422-8435 | Species | denotes | coronaviruses | Tax:11118 |
| 345 | 8767-8775 | Species | denotes | SARS-CoV | Tax:694009 |
| 346 | 8436-8440 | Species | denotes | 229E | Tax:11137 |
| 347 | 8442-8446 | Species | denotes | NL63 | Tax:277944 |
| 348 | 8448-8452 | Species | denotes | OC43 | Tax:31631 |
| 349 | 8457-8461 | Species | denotes | HKU1 | Tax:290028 |
| 350 | 8360-8392 | Disease | denotes | Middle East respiratory syndrome | MESH:D018352 |
| 351 | 8394-8398 | Disease | denotes | MERS | MESH:D018352 |
| 354 | 9164-9174 | Species | denotes | SARS-CoV-2 | Tax:2697049 |
| 355 | 9244-9249 | Species | denotes | human | Tax:9606 |
LitCovid-PD-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T104 | 1230-1238 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T105 | 1230-1234 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T106 | 1572-1580 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T107 | 1572-1576 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T108 | 1713-1724 | Disease | denotes | hepatitis B | http://purl.obolibrary.org/obo/MONDO_0005344 |
| T109 | 1713-1722 | Disease | denotes | hepatitis | http://purl.obolibrary.org/obo/MONDO_0002251 |
| T110 | 4286-4294 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T111 | 4286-4290 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T112 | 5708-5710 | Disease | denotes | VL | http://purl.obolibrary.org/obo/MONDO_0024555 |
| T113 | 6479-6487 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T114 | 6479-6483 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T115 | 7335-7350 | Disease | denotes | Virus infection | http://purl.obolibrary.org/obo/MONDO_0005108 |
| T116 | 7341-7350 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
| T117 | 7526-7534 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T118 | 7526-7530 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T119 | 7538-7546 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T120 | 7538-7542 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T121 | 7629-7638 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
| T122 | 7711-7720 | Disease | denotes | infection | http://purl.obolibrary.org/obo/MONDO_0005550 |
| T123 | 8325-8333 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T124 | 8325-8329 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T125 | 8337-8345 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T126 | 8337-8341 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T127 | 8410-8421 | Disease | denotes | common-cold | http://purl.obolibrary.org/obo/MONDO_0005709 |
| T128 | 8767-8775 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T129 | 8767-8771 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T130 | 9164-9172 | Disease | denotes | SARS-CoV | http://purl.obolibrary.org/obo/MONDO_0005091 |
| T131 | 9164-9168 | Disease | denotes | SARS | http://purl.obolibrary.org/obo/MONDO_0005091 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T74 | 9-14 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T75 | 15-19 | http://purl.obolibrary.org/obo/CLO_0009524 | denotes | Vero |
| T76 | 15-19 | http://purl.obolibrary.org/obo/CLO_0050515 | denotes | Vero |
| T77 | 27-38 | http://purl.obolibrary.org/obo/CLO_0051656 | denotes | COS-7 cells |
| T78 | 27-38 | http://purl.obolibrary.org/obo/CLO_0051657 | denotes | COS-7 cells |
| T79 | 27-38 | http://purl.obolibrary.org/obo/CLO_0051658 | denotes | COS-7 cells |
| T80 | 27-32 | http://purl.obolibrary.org/obo/CLO_0002597 | denotes | COS-7 |
| T81 | 27-32 | http://purl.obolibrary.org/obo/CLO_0050508 | denotes | COS-7 |
| T82 | 405-410 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T83 | 617-622 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T84 | 706-715 | http://purl.obolibrary.org/obo/CLO_0036932 | denotes | hybridoma |
| T85 | 787-791 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T86 | 836-838 | http://purl.obolibrary.org/obo/CLO_0003797 | denotes | HP |
| T87 | 836-838 | http://purl.obolibrary.org/obo/PR_000008725 | denotes | HP |
| T88 | 1309-1313 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | gene |
| T89 | 1506-1507 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T90 | 1723-1724 | http://purl.obolibrary.org/obo/CLO_0001021 | denotes | B |
| T91 | 1725-1730 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T92 | 1764-1765 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T93 | 1840-1845 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T94 | 1932-1934 | http://purl.obolibrary.org/obo/CLO_0003797 | denotes | HP |
| T95 | 1932-1934 | http://purl.obolibrary.org/obo/PR_000008725 | denotes | HP |
| T96 | 2061-2066 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T97 | 2171-2176 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T98 | 2341-2345 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T99 | 2370-2374 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T100 | 2389-2393 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T101 | 2476-2480 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T102 | 2598-2606 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membrane |
| T103 | 2651-2659 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membrane |
| T104 | 2819-2823 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T105 | 2845-2853 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membrane |
| T106 | 2898-2899 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T107 | 3008-3016 | http://purl.obolibrary.org/obo/UBERON_0000158 | denotes | membrane |
| T108 | 3288-3299 | http://purl.obolibrary.org/obo/CLO_0051656 | denotes | COS-7 cells |
| T109 | 3288-3299 | http://purl.obolibrary.org/obo/CLO_0051657 | denotes | COS-7 cells |
| T110 | 3288-3299 | http://purl.obolibrary.org/obo/CLO_0051658 | denotes | COS-7 cells |
| T111 | 3288-3293 | http://purl.obolibrary.org/obo/CLO_0002597 | denotes | COS-7 |
| T112 | 3288-3293 | http://purl.obolibrary.org/obo/CLO_0050508 | denotes | COS-7 |
| T113 | 3494-3496 | http://purl.obolibrary.org/obo/CLO_0009141 | denotes | St |
| T114 | 3494-3496 | http://purl.obolibrary.org/obo/CLO_0050980 | denotes | St |
| T115 | 3505-3507 | http://purl.obolibrary.org/obo/CLO_0007815 | denotes | MO |
| T116 | 3541-3546 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T117 | 3611-3616 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T118 | 3688-3690 | http://purl.obolibrary.org/obo/CLO_0053799 | denotes | 45 |
| T119 | 3727-3731 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T120 | 3737-3742 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T121 | 4103-4106 | http://purl.obolibrary.org/obo/CL_0000784 | denotes | iPC |
| T122 | 4107-4116 | http://purl.obolibrary.org/obo/BFO_0000030 | denotes | objective |
| T123 | 4117-4121 | http://purl.obolibrary.org/obo/UBERON_0005389 | denotes | lens |
| T124 | 4437-4439 | http://purl.obolibrary.org/obo/CLO_0001302 | denotes | 34 |
| T125 | 4455-4456 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T126 | 4519-4520 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T127 | 4594-4598 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T128 | 4923-4927 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T129 | 4933-4938 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T130 | 5340-5341 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T131 | 5547-5552 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
| T132 | 5581-5582 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T133 | 5623-5625 | http://purl.obolibrary.org/obo/CLO_0050507 | denotes | 22 |
| T134 | 5748-5753 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | genes |
| T135 | 5782-5786 | http://purl.obolibrary.org/obo/OGG_0000000002 | denotes | gene |
| T136 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0001230 | denotes | 293 |
| T137 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0037237 | denotes | 293 |
| T138 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0050903 | denotes | 293 |
| T139 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0054249 | denotes | 293 |
| T140 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0054250 | denotes | 293 |
| T141 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0054251 | denotes | 293 |
| T142 | 5987-5990 | http://purl.obolibrary.org/obo/CLO_0054252 | denotes | 293 |
| T143 | 5991-5996 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T144 | 6208-6210 | http://purl.obolibrary.org/obo/CLO_0001302 | denotes | 34 |
| T145 | 6281-6282 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T146 | 6330-6334 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
| T147 | 6767-6768 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T148 | 6880-6884 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T149 | 6890-6899 | http://purl.obolibrary.org/obo/CLO_0053695 | denotes | human IgG |
| T150 | 7114-7115 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T151 | 7204-7210 | http://purl.obolibrary.org/obo/UBERON_0002415 | denotes | tailed |
| T152 | 7223-7227 | http://purl.obolibrary.org/obo/UBERON_0000473 | denotes | test |
| T153 | 7335-7340 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | Virus |
| T154 | 7394-7399 | http://purl.obolibrary.org/obo/NCBITaxon_10239 | denotes | virus |
| T155 | 7493-7506 | http://purl.obolibrary.org/obo/CLO_0051719 | denotes | Vero-E6 cells |
| T156 | 7556-7561 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
| T157 | 7611-7612 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T158 | 7722-7727 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T159 | 7777-7782 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T160 | 7814-7819 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T161 | 7945-7950 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T162 | 8044-8048 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T163 | 8054-8059 | http://purl.obolibrary.org/obo/CLO_0007836 | denotes | mouse |
| T164 | 8155-8160 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | Cells |
| T165 | 8404-8409 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
| T166 | 8541-8542 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | A |
| T167 | 9244-9249 | http://purl.obolibrary.org/obo/NCBITaxon_9606 | denotes | human |
| T168 | 9755-9756 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T46 | 107-109 | Chemical | denotes | VA | http://purl.obolibrary.org/obo/CHEBI_75008 |
| T47 | 218-220 | Chemical | denotes | MA | http://purl.obolibrary.org/obo/CHEBI_474859|http://purl.obolibrary.org/obo/CHEBI_73610|http://purl.obolibrary.org/obo/CHEBI_90325 |
| T50 | 333-343 | Chemical | denotes | penicillin | http://purl.obolibrary.org/obo/CHEBI_17334|http://purl.obolibrary.org/obo/CHEBI_51356 |
| T52 | 358-370 | Chemical | denotes | streptomycin | http://purl.obolibrary.org/obo/CHEBI_17076|http://purl.obolibrary.org/obo/CHEBI_58007 |
| T54 | 530-540 | Chemical | denotes | penicillin | http://purl.obolibrary.org/obo/CHEBI_17334|http://purl.obolibrary.org/obo/CHEBI_51356 |
| T56 | 552-564 | Chemical | denotes | streptomycin | http://purl.obolibrary.org/obo/CHEBI_17076|http://purl.obolibrary.org/obo/CHEBI_58007 |
| T58 | 579-588 | Chemical | denotes | geneticin | http://purl.obolibrary.org/obo/CHEBI_42768 |
| T59 | 656-659 | Chemical | denotes | CO2 | http://purl.obolibrary.org/obo/CHEBI_16526 |
| T60 | 826-833 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T61 | 836-838 | Chemical | denotes | HP | http://purl.obolibrary.org/obo/CHEBI_74055 |
| T62 | 857-859 | Chemical | denotes | GE | http://purl.obolibrary.org/obo/CHEBI_73801 |
| T63 | 881-883 | Chemical | denotes | IL | http://purl.obolibrary.org/obo/CHEBI_63895|http://purl.obolibrary.org/obo/CHEBI_74072 |
| T65 | 961-984 | Chemical | denotes | sodium dodecyl sulphate | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T66 | 961-967 | Chemical | denotes | sodium | http://purl.obolibrary.org/obo/CHEBI_26708 |
| T67 | 968-975 | Chemical | denotes | dodecyl | http://purl.obolibrary.org/obo/CHEBI_23870 |
| T68 | 976-984 | Chemical | denotes | sulphate | http://purl.obolibrary.org/obo/CHEBI_16189 |
| T69 | 985-999 | Chemical | denotes | polyacrylamide | http://purl.obolibrary.org/obo/CHEBI_51135|http://purl.obolibrary.org/obo/CHEBI_53656|http://purl.obolibrary.org/obo/CHEBI_60766 |
| T72 | 1021-1024 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T73 | 1119-1126 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T74 | 1133-1140 | Chemical | denotes | reagent | http://purl.obolibrary.org/obo/CHEBI_33893 |
| T75 | 1208-1215 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T76 | 1243-1250 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T77 | 1464-1471 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T78 | 1521-1528 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T79 | 1736-1743 | Chemical | denotes | antigen | http://purl.obolibrary.org/obo/CHEBI_59132 |
| T80 | 1932-1934 | Chemical | denotes | HP | http://purl.obolibrary.org/obo/CHEBI_74055 |
| T81 | 1935-1938 | Chemical | denotes | DNA | http://purl.obolibrary.org/obo/CHEBI_16991 |
| T82 | 1952-1959 | Chemical | denotes | reagent | http://purl.obolibrary.org/obo/CHEBI_33893 |
| T83 | 2132-2141 | Chemical | denotes | phosphate | http://purl.obolibrary.org/obo/CHEBI_18367|http://purl.obolibrary.org/obo/CHEBI_26020|http://purl.obolibrary.org/obo/CHEBI_35780|http://purl.obolibrary.org/obo/CHEBI_43474 |
| T87 | 2220-2226 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T88 | 2287-2294 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T89 | 2548-2551 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T90 | 2583-2597 | Chemical | denotes | nitrocellulose | http://purl.obolibrary.org/obo/CHEBI_53325 |
| T91 | 2694-2698 | Chemical | denotes | Tris | http://purl.obolibrary.org/obo/CHEBI_9754 |
| T92 | 3165-3167 | Chemical | denotes | MP | http://purl.obolibrary.org/obo/CHEBI_73612 |
| T93 | 3465-3477 | Chemical | denotes | Triton X-100 | http://purl.obolibrary.org/obo/CHEBI_9750 |
| T94 | 3700-3715 | Chemical | denotes | Alexa Fluor 488 | http://purl.obolibrary.org/obo/CHEBI_52661 |
| T95 | 3706-3711 | Chemical | denotes | Fluor | http://purl.obolibrary.org/obo/CHEBI_24061 |
| T96 | 3874-3886 | Chemical | denotes | phenylindole | http://purl.obolibrary.org/obo/CHEBI_48559 |
| T97 | 3888-3892 | Chemical | denotes | DAPI | http://purl.obolibrary.org/obo/CHEBI_51231 |
| T98 | 3936-3940 | Chemical | denotes | Gold | http://purl.obolibrary.org/obo/CHEBI_29287 |
| T99 | 4299-4306 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T100 | 4415-4421 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T101 | 4429-4435 | Chemical | denotes | NaHCO3 | http://purl.obolibrary.org/obo/CHEBI_32139 |
| T102 | 4443-4449 | Chemical | denotes | Na2CO3 | http://purl.obolibrary.org/obo/CHEBI_29377 |
| T103 | 4475-4482 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T104 | 4788-4794 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T105 | 5011-5017 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T106 | 5270-5284 | Chemical | denotes | sulphuric acid | http://purl.obolibrary.org/obo/CHEBI_26836 |
| T107 | 5280-5284 | Chemical | denotes | acid | http://purl.obolibrary.org/obo/CHEBI_37527 |
| T108 | 5648-5650 | Chemical | denotes | VH | http://purl.obolibrary.org/obo/CHEBI_73700 |
| T109 | 5708-5710 | Chemical | denotes | VL | http://purl.obolibrary.org/obo/CHEBI_75013 |
| T110 | 6063-6070 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T111 | 6186-6192 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T112 | 6200-6206 | Chemical | denotes | NaHCO3 | http://purl.obolibrary.org/obo/CHEBI_32139 |
| T113 | 6214-6220 | Chemical | denotes | Na2CO3 | http://purl.obolibrary.org/obo/CHEBI_29377 |
| T114 | 6492-6499 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T115 | 6574-6576 | Chemical | denotes | HA | http://purl.obolibrary.org/obo/CHEBI_73924 |
| T116 | 7068-7082 | Chemical | denotes | sulphuric acid | http://purl.obolibrary.org/obo/CHEBI_26836 |
| T117 | 7078-7082 | Chemical | denotes | acid | http://purl.obolibrary.org/obo/CHEBI_37527 |
| T118 | 7752-7760 | Chemical | denotes | methanol | http://purl.obolibrary.org/obo/CHEBI_17790 |
| T119 | 7761-7768 | Chemical | denotes | acetone | http://purl.obolibrary.org/obo/CHEBI_15347 |
| T120 | 7889-7895 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T121 | 8017-8032 | Chemical | denotes | Alexa Fluor 488 | http://purl.obolibrary.org/obo/CHEBI_52661 |
| T122 | 8023-8028 | Chemical | denotes | Fluor | http://purl.obolibrary.org/obo/CHEBI_24061 |
| T123 | 8106-8112 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T124 | 8124-8128 | Chemical | denotes | DAPI | http://purl.obolibrary.org/obo/CHEBI_51231 |
| T125 | 8293-8300 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T126 | 8873-8877 | Chemical | denotes | MEGA | http://purl.obolibrary.org/obo/CHEBI_6617 |
| T127 | 8913-8918 | Chemical | denotes | amino | http://purl.obolibrary.org/obo/CHEBI_46882 |
| T128 | 8919-8923 | Chemical | denotes | acid | http://purl.obolibrary.org/obo/CHEBI_37527 |
| T129 | 9016-9023 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T130 | 9101-9108 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T131 | 9128-9138 | Chemical | denotes | amino acid | http://purl.obolibrary.org/obo/CHEBI_33709 |
| T132 | 9128-9133 | Chemical | denotes | amino | http://purl.obolibrary.org/obo/CHEBI_46882 |
| T133 | 9134-9138 | Chemical | denotes | acid | http://purl.obolibrary.org/obo/CHEBI_37527 |
| T134 | 9203-9210 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T135 | 9638-9645 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T136 | 9689-9696 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T137 | 9701-9706 | Chemical | denotes | amino | http://purl.obolibrary.org/obo/CHEBI_46882 |
| T138 | 9707-9711 | Chemical | denotes | acid | http://purl.obolibrary.org/obo/CHEBI_37527 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 1314-1323 | http://purl.obolibrary.org/obo/GO_0009058 | denotes | synthesis |
| T2 | 2900-2928 | http://purl.obolibrary.org/obo/GO_0004601 | denotes | horseradish peroxidase (HRP) |
| T3 | 5787-5796 | http://purl.obolibrary.org/obo/GO_0009058 | denotes | synthesis |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T41 | 0-7 | Sentence | denotes | Methods |
| T42 | 9-14 | Sentence | denotes | Cells |
| T43 | 15-398 | Sentence | denotes | Vero E6 and COS-7 cells were purchased from the American Type Culture Collection (Manassas, VA, United States) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham MA, United States) supplemented with 10% fetal bovine serum (FBS; HyClone, Logan, UT, United States), 100 units/mL penicillin and 100 µg/mL streptomycin (Thermo Fisher Scientific). |
| T44 | 399-616 | Sentence | denotes | 293FT cells were purchased from Invitrogen (Carlsbad, CA, United States) and grown in DMEM supplemented with 10% FBS, 100 units/mL penicillin, 100 µg/mL streptomycin and 500 µg/mL geneticin (Thermo Fisher Scientific). |
| T45 | 617-660 | Sentence | denotes | Cells were maintained at 37 °C with 5% CO2. |
| T46 | 662-701 | Sentence | denotes | Purification of monoclonal antibody 1A9 |
| T47 | 702-758 | Sentence | denotes | The hybridoma for mAb 1A9 was previously generated [20]. |
| T48 | 759-921 | Sentence | denotes | All mAbs were purified from cell culture supernatants using HiTrap protein G HP affinity columns (GE Healthcare, Chicago, IL, United States) and stored at −80 °C. |
| T49 | 922-1040 | Sentence | denotes | The purity of the mAb was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoretic (SDS-PAGE) analysis. |
| T50 | 1041-1168 | Sentence | denotes | The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Fisher Scientific). |
| T51 | 1170-1229 | Sentence | denotes | Plasmids for expression of full spike protein and fragments |
| T52 | 1230-1410 | Sentence | denotes | SARS-CoV-2 S-protein-expressing plasmids were codon-optimised and generated by gene synthesis (Bio Basic Asia Pacific, Singapore) according to GenBank accession number: QHD43416.1. |
| T53 | 1411-1594 | Sentence | denotes | One plasmid is for expressing untagged full-length S protein while the other is for expressing a Myc-tagged S-protein fragment consisting of residues 1048–1206 (SARS-CoV-2 numbering). |
| T54 | 1595-1783 | Sentence | denotes | The pXJ40-Myc expression vector was used as an empty vector control and pXJ40-Myc-HBcAg plasmid expressing Myc-tagged hepatitis B virus core antigen (HBcAg) was used as a negative control. |
| T55 | 1785-1833 | Sentence | denotes | Transient transfection and western blot analysis |
| T56 | 1834-2029 | Sentence | denotes | 293FT cells were seeded onto 6-cm dishes 24 hours before transient transfection using X-tremeGENE HP DNA transfection reagent (Roche, Basel, Switzerland) according to the manufacturer’s protocol. |
| T57 | 2030-2170 | Sentence | denotes | At 24 hours post-transfection, cells were harvested, spun down by centrifugation and washed with cold phosphate buffered saline (PBS) twice. |
| T58 | 2171-2249 | Sentence | denotes | Cells were then resuspended in 2× Laemmli sample buffer, boiled and sonicated. |
| T59 | 2250-2452 | Sentence | denotes | Clarified supernatant containing the protein of interest was obtained by spinning down the cell lysate at 13,000 rpm at 4 °C to remove the cell debris and further analysed by western blot (WB) analysis. |
| T60 | 2453-2646 | Sentence | denotes | Equal amounts of total cell lysates were loaded per lane and resolved using electrophoresis on SDS-PAGE gels and transferred onto nitrocellulose membrane (Bio-Rad, Hercules, CA, United States). |
| T61 | 2647-2834 | Sentence | denotes | The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST) for 1 hour at room temperature (RT) and incubated with primary antibodies at 4 °C overnight. |
| T62 | 2835-3003 | Sentence | denotes | After the membrane was washed with TBST, it was incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (Thermo Fisher Scientific) at RT for 1 hour. |
| T63 | 3004-3193 | Sentence | denotes | The membrane was then washed with TBST again and bound antibodies visualised with enhanced chemiluminescence substrate (Thermo Fisher Scientific) using ChemiDoc MP Imaging System (Bio-Rad). |
| T64 | 3195-3249 | Sentence | denotes | Transient transfection and immunofluorescence analysis |
| T65 | 3250-3534 | Sentence | denotes | For immunofluorescence (IF) analysis, COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at RT followed by permeabilisation with 0.2% Triton X-100 (Sigma-Aldrich, St. Louis, MO, United States) for 5 min. |
| T66 | 3535-3610 | Sentence | denotes | Fixed cells were then blocked with PBS containing 10% FBS for 30 min at RT. |
| T67 | 3611-3805 | Sentence | denotes | Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies, Carlsbad, CA, United States). |
| T68 | 3806-4005 | Sentence | denotes | Immunolabelled coverslips were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich), and mounted using ProLong Gold Antifade Mountant (Molecular Probes, Eugene, OR, United States). |
| T69 | 4006-4184 | Sentence | denotes | Images were acquired with Olympus CKX53 microscope using Olympus (Tokyo, Japan) LCAch N 20×/0.40 iPC objective lens and Olympus DP27 colour camera with Olympus cellSens software. |
| T70 | 4185-4258 | Sentence | denotes | Each channel was collected separately, with images at 1024 × 1024 pixels. |
| T71 | 4260-4265 | Sentence | denotes | ELISA |
| T72 | 4266-4376 | Sentence | denotes | Whole ectodomain of SARS-CoV-2 S protein with His Tag (Sino Biological Inc., Beijing, China; catalogue number: |
| T73 | 4377-4599 | Sentence | denotes | 40589-B08V1) was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and a total of 20 ng of protein was loaded into individual wells of a 96 well plate (Nunc, Roskilde, Denmark) and allowed to coat overnight at 4 °C. |
| T74 | 4600-4834 | Sentence | denotes | Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour. |
| T75 | 4835-5051 | Sentence | denotes | Plate were washed four times with PBST before incubation for 1 hour with HRP-conjugated goat anti-mouse IgG (Thermo Fisher Scientific) secondary antibodies diluted in blocking buffer, and washed four times with PBST. |
| T76 | 5052-5285 | Sentence | denotes | Visualisation of bound secondary antibodies was done by the addition of 3,3',5,5'-tetramethylbenzidine (TMB) substrate (Thermo Fisher Scientific) for 5 min in the absence of light and the reaction was stopped with 2 M sulphuric acid. |
| T77 | 5286-5500 | Sentence | denotes | Optical density at 450 nm (OD450nm) was determined by a Tecan (Männedorf, Switzerland) Infinite M1000 reader and normalised OD450nm was obtained by subtracting background absorbances determined in BSA coated wells. |
| T78 | 5502-5542 | Sentence | denotes | Production of monoclonal antibody CR3022 |
| T79 | 5543-5627 | Sentence | denotes | The human mAb CR3022 was expressed in a similar manner as previously described [22]. |
| T80 | 5628-5949 | Sentence | denotes | The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. |
| T81 | 5950-6136 | Sentence | denotes | Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]. |
| T82 | 6138-6152 | Sentence | denotes | Sandwich ELISA |
| T83 | 6153-6335 | Sentence | denotes | Mab 1A9 was diluted with coating buffer (0.1 M NaHCO3, 34 mM Na2CO3) and 0.1 µg of antibody was coated onto individual wells of a Maxisorp flat-bottom plate (Nunc) overnight at 4 °C. |
| T84 | 6336-6442 | Sentence | denotes | The plate was washed three times with PBST before blocking was done using 5% BSA/PBST at 37 °C for 60 min. |
| T85 | 6443-6540 | Sentence | denotes | Dilutions of His-tagged full length SARS-CoV-2 S protein (Sino Biological Inc., catalogue number: |
| T86 | 6541-6617 | Sentence | denotes | 40589-B08V1) and His-tagged H7N7-HA (Sino Biological Inc., catalogue number: |
| T87 | 6618-6727 | Sentence | denotes | 11082-V08B) were added to desired wells and incubated at 37 °C for 90 min followed by three washes with PBST. |
| T88 | 6728-6957 | Sentence | denotes | 100 µL of CR3022 antibody was added at a concentration of 1 µg/mL and incubated at 37 °C for 60 min followed by three PBST washes before HRP-conjugated goat anti-human IgG (Thermo Fisher Scientific) was added for 60 min at 37 °C. |
| T89 | 6958-7083 | Sentence | denotes | Finally, after three PBST washes, TMB (Sigma-Aldrich) was added for 5 min and the reaction was stopped by 2 M sulphuric acid. |
| T90 | 7084-7144 | Sentence | denotes | The OD450nm was determined by a Tecan Infinite M1000 reader. |
| T91 | 7145-7333 | Sentence | denotes | Statistical analyses were performed using an unpaired, one-tailed Student’s t-test with Welch’s correction for unequal variances. p values < 0.05 were considered statistically significant. |
| T92 | 7335-7373 | Sentence | denotes | Virus infection and immunofluorescence |
| T93 | 7374-7492 | Sentence | denotes | All works with live virus were performed in the biosafety level (BSL)3 facility at the Public Health Agency of Sweden. |
| T94 | 7493-7694 | Sentence | denotes | Vero-E6 cells were infected with SARS-CoV-2 (SARS-CoV-2-Iso/01/human/2020/SWE; GenBank accession number: MT093571) at a multiplicity of infection (MOI) of one in DMEM 2% FBS (Thermo Fisher Scientific). |
| T95 | 7695-7813 | Sentence | denotes | At 24 hour post-infection, cells were fixed with chilled methanol/acetone and the cells were kept at −20 °C overnight. |
| T96 | 7814-7940 | Sentence | denotes | Cells were then stained using mAb 1A9 at 5 µg/mL at 37 °C for 30 min in IF buffer (BSA 0.2%, Triton ×100 0.1% in PBS, pH 7.4). |
| T97 | 7941-8154 | Sentence | denotes | The cells were washed three times with PBS and incubated, subsequently with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher Scientific) in IF buffer containing DAPI for an additional 30 min. |
| T98 | 8155-8265 | Sentence | denotes | Cells were washed three times with PBS before visualisation and image acquisition with fluorescent microscopy. |
| T99 | 8267-8290 | Sentence | denotes | Bioinformatics analysis |
| T100 | 8291-8540 | Sentence | denotes | S protein reference sequences for SARS-CoV-1, SARS-CoV-2, batRaTG13, Middle East respiratory syndrome (MERS) and human common-cold coronaviruses 229E, NL63, OC43 and HKU1 were downloaded from the National Center for Biotechnology Information (NCBI). |
| T101 | 8541-8822 | Sentence | denotes | A multiple sequence alignment was created with multiple alignment using fast Fourier transform (MAFFT) using the slow but accurate L-INS-I parameter settings [24] and the alignment curated, cut to the target region 1029–1192 (SARS-CoV-1 numbering) and visualised with Jalview [25]. |
| T102 | 8823-9150 | Sentence | denotes | We used Molecular Evolutionary Genetics Analysis (MEGA) X [26] to calculate the number of amino-acid differences for all sequence pairs in the alignment of the mAb target region and the full S protein normalised by the length of the aligned sequence of the respective reference protein to obtain per cent amino acid identities. |
| T103 | 9151-9346 | Sentence | denotes | To determine SARS-CoV-2 sequence diversity in the S protein within the current pandemic, 230 human and environmental viral sequences were downloaded from GISAID’s EpiCoV database on 1 March 2020. |
| T104 | 9347-9487 | Sentence | denotes | We gratefully acknowledge the authors, originating and submitting laboratories of the sequences on which this part of the research is based. |
| T105 | 9488-9534 | Sentence | denotes | The list is detailed in Supplementary Table 1. |
| T106 | 9535-9646 | Sentence | denotes | The nt sequences were searched with basic local alignment search tool (BLAST)X against the reference S protein. |
| T107 | 9647-9800 | Sentence | denotes | 174 hits covered the full length of the S protein and amino-acid mutations were counted and tabulated using a custom Perl script (Supplementary Table 2). |
| T108 | 9802-9819 | Sentence | denotes | Ethical statement |
| T109 | 9820-9869 | Sentence | denotes | Ethical approval was not required for this study. |
LitCovid-PD-HP
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 1713-1722 | Phenotype | denotes | hepatitis | http://purl.obolibrary.org/obo/HP_0012115 |
2_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 32700671-16378996-29327476 | 754-756 | 16378996 | denotes | 20 |
| 32700671-16796401-29327477 | 5623-5625 | 16796401 | denotes | 22 |
| 32700671-31906790-29327478 | 6132-6134 | 31906790 | denotes | 23 |
| 32700671-29506019-29327479 | 8700-8702 | 29506019 | denotes | 24 |
| 32700671-19151095-29327480 | 8818-8820 | 19151095 | denotes | 25 |
| 32700671-29722887-29327481 | 8882-8884 | 29722887 | denotes | 26 |
MyTest
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 32700671-16378996-29327476 | 754-756 | 16378996 | denotes | 20 |
| 32700671-16796401-29327477 | 5623-5625 | 16796401 | denotes | 22 |
| 32700671-31906790-29327478 | 6132-6134 | 31906790 | denotes | 23 |
| 32700671-29506019-29327479 | 8700-8702 | 29506019 | denotes | 24 |
| 32700671-19151095-29327480 | 8818-8820 | 19151095 | denotes | 25 |
| 32700671-29722887-29327481 | 8882-8884 | 29722887 | denotes | 26 |