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PMC:7376845 / 24734-27108 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T175 22-30 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T176 54-61 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T177 116-121 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T178 191-198 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T179 430-437 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T180 506-514 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T181 549-557 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T182 571-578 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T183 628-635 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T184 748-755 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T185 818-825 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T186 902-910 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T187 938-945 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T188 1001-1006 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T189 1069-1077 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T190 1331-1338 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T191 1428-1435 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T192 1703-1711 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T193 1734-1741 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T194 1800-1805 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T195 1922-1927 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T196 2013-2021 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T197 2159-2164 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T198 2250-2255 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T199 2349-2356 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
614 52-61 Gene denotes S protein
615 96-115 Disease denotes SARS-CoV-2 infected MESH:C000657245
621 320-325 Species denotes human Tax:9606
622 381-391 Species denotes SARS-CoV-2 Tax:2697049
623 639-656 Species denotes influenza A virus Tax:11320
624 330-336 Chemical denotes CR3022
625 519-525 Chemical denotes CR3022
627 981-1000 Disease denotes SARS-CoV-2 infected MESH:C000657245
634 1027-1031 Species denotes H7N7 Tax:119218
635 1132-1142 Species denotes SARS-CoV-2 Tax:2697049
636 1144-1191 Species denotes severe acute respiratory syndrome coronavirus 2 Tax:2697049
637 1033-1042 Species denotes influenza Tax:11320
638 1046-1050 Species denotes H7N7 Tax:119218
639 1100-1109 Disease denotes infection MESH:D007239
648 1263-1268 Species denotes human Tax:9606
649 1314-1324 Species denotes SARS-CoV-2 Tax:2697049
650 1452-1456 Species denotes H7N7 Tax:119218
651 1721-1731 Species denotes SARS-CoV-2 Tax:2697049
652 1767-1771 Species denotes H7N7 Tax:119218
653 1439-1448 Species denotes influenza Tax:11320
654 1754-1763 Species denotes influenza Tax:11320
655 1273-1279 Chemical denotes CR3022
663 1855-1865 Species denotes SARS-CoV-2 Tax:2697049
664 1976-1991 Chemical denotes Alexa Fluor 488
665 2063-2092 Chemical denotes 4′,6-diamidino-2-phenylindole MESH:C007293
666 2094-2098 Chemical denotes DAPI MESH:C007293
667 1816-1824 Disease denotes infected MESH:D007239
668 1841-1849 Disease denotes infected MESH:D007239
669 1907-1916 Disease denotes infection MESH:D007239
675 2136-2150 Disease denotes CoV-2-infected MESH:C000657245
676 2230-2249 Disease denotes SARS-CoV-2-infected MESH:C000657245
677 2273-2282 Disease denotes infection MESH:D007239
678 2364-2373 Disease denotes infection MESH:D007239
679 2151-2158 CellLine denotes Vero-E6 CVCL:0574

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T219 96-104 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T220 96-100 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T221 381-389 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T222 381-385 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T223 639-648 Disease denotes influenza http://purl.obolibrary.org/obo/MONDO_0005812
T224 981-989 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T225 981-985 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T226 1033-1042 Disease denotes influenza http://purl.obolibrary.org/obo/MONDO_0005812
T227 1100-1109 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T228 1111-1113 Disease denotes OD http://purl.obolibrary.org/obo/MONDO_0017178
T229 1132-1140 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T230 1132-1136 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T231 1144-1191 Disease denotes severe acute respiratory syndrome coronavirus 2 http://purl.obolibrary.org/obo/MONDO_0100096
T232 1144-1177 Disease denotes severe acute respiratory syndrome http://purl.obolibrary.org/obo/MONDO_0005091
T233 1314-1322 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T234 1314-1318 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T235 1439-1448 Disease denotes influenza http://purl.obolibrary.org/obo/MONDO_0005812
T236 1721-1729 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T237 1721-1725 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T238 1754-1763 Disease denotes influenza http://purl.obolibrary.org/obo/MONDO_0005812
T239 1855-1863 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T240 1855-1859 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T241 1907-1916 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T242 2131-2139 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T243 2131-2135 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T244 2230-2238 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T245 2230-2234 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T246 2273-2282 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550
T247 2364-2373 Disease denotes infection http://purl.obolibrary.org/obo/MONDO_0005550

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T264 65-66 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T265 116-121 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T266 160-163 http://purl.obolibrary.org/obo/CLO_0051582 denotes has
T267 247-248 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T268 320-325 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T269 367-369 http://purl.obolibrary.org/obo/CLO_0050050 denotes S1
T270 496-497 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T271 538-539 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T272 595-596 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T273 649-650 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T274 651-656 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T275 933-934 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T276 949-950 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T277 978-979 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T278 1001-1006 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T279 1043-1044 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T280 1193-1194 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T281 1263-1268 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T282 1301-1302 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T283 1449-1450 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T284 1458-1463 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T285 1476-1477 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T286 1764-1765 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T287 1789-1790 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T288 1792-1805 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero E6 cells
T289 1922-1927 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T290 2121-2127 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested
T291 2151-2164 http://purl.obolibrary.org/obo/CLO_0051719 denotes Vero-E6 cells
T292 2205-2206 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T293 2250-2255 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T218 54-61 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T219 191-198 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T220 430-437 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T221 571-578 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T222 624-626 Chemical denotes HA http://purl.obolibrary.org/obo/CHEBI_73924
T223 628-635 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T224 748-755 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T225 811-813 Chemical denotes HA http://purl.obolibrary.org/obo/CHEBI_73924
T226 818-825 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T227 938-945 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T228 1007-1009 Chemical denotes HA http://purl.obolibrary.org/obo/CHEBI_73924
T229 1331-1338 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T230 1425-1427 Chemical denotes HA http://purl.obolibrary.org/obo/CHEBI_73924
T231 1428-1435 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T232 1734-1741 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T233 1773-1775 Chemical denotes HA http://purl.obolibrary.org/obo/CHEBI_73924
T234 1976-1991 Chemical denotes Alexa Fluor 488 http://purl.obolibrary.org/obo/CHEBI_52661
T235 1982-1987 Chemical denotes Fluor http://purl.obolibrary.org/obo/CHEBI_24061
T236 2080-2092 Chemical denotes phenylindole http://purl.obolibrary.org/obo/CHEBI_48559
T237 2094-2098 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T238 2349-2356 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T192 0-121 Sentence denotes Utility of monoclonal antibody 1A9 for detection of S protein in a sandwich ELISA format and in SARS-CoV-2 infected cells
T193 122-239 Sentence denotes Based on indirect ELISA data, mAb 1A9 has the strongest binding to S protein when compared with the other three mAbs.
T194 240-392 Sentence denotes Hence, a sandwich ELISA was performed to determine if it can be paired with the human mAb CR3022 which is known to bind to the S1 subunit of SARS-CoV-2.
T195 393-558 Sentence denotes As shown in Figure 4A, recombinant S protein was detected at 15.6 ng/mL and above when 1A9 was used as a capture antibody and CR3022 was used as a detector antibody.
T196 559-702 Sentence denotes Since the S protein was His-tagged, a His-tagged haemagglutinin (HA) protein of influenza A virus was used to check for specificity of binding.
T197 703-865 Sentence denotes The absorbance readings in the presence of S protein were significantly higher than that in the presence of HA for protein concentrations of 15.6 ng/mL and above.
T198 866-1006 Sentence denotes Figure 4 Performance of monoclonal antibody 1A9 for detection of (A) S protein in a sandwich ELISA format and (B) SARS-CoV-2 infected cells
T199 1007-1192 Sentence denotes HA: haemagglutinin; H7N7: influenza A (H7N7); mAb: monoclonal antibody; MOI: multiplicity of infection; OD: optical density; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2.
T200 1193-1195 Sentence denotes A.
T201 1196-1339 Sentence denotes Sandwich ELISA assay to determine mAb 1A9 ability to pair with the human mAb CR3022 for the detection of a His-tagged SARS-CoV-2 spike protein.
T202 1340-1413 Sentence denotes 1A9 and CR3022 were used as capture and detector antibodies respectively.
T203 1414-1495 Sentence denotes His-tagged HA protein of influenza A (H7N7) virus was used as a negative control.
T204 1496-1563 Sentence denotes Averaged readings across three replicate experiments are presented.
T205 1564-1644 Sentence denotes Error bars represent standard deviations across the three replicate experiments.
T206 1645-1788 Sentence denotes Asterisks indicate significantly increased binding of the antibody pairs to SARS-CoV-2 S protein compared to influenza A (H7N7) HA at p < 0.05.
T207 1789-1791 Sentence denotes B.
T208 1792-1890 Sentence denotes Vero E6 cells were mock-infected (left panel) or infected with SARS-CoV-2 (right panel; MOI of 1).
T209 1891-2030 Sentence denotes At 24 hour post infection, the cells were stained with mAb 1A9 (5 µg/mL) followed by Alexa Fluor 488-conjugated secondary antibody (green).
T210 2031-2106 Sentence denotes Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue).
T211 2107-2165 Sentence denotes Next, 1A9 was tested on SARS-CoV-2-infected Vero-E6 cells.
T212 2166-2374 Sentence denotes As shown in Figure 4B, mAb 1A9 stained a considerable number of SARS-CoV-2-infected cells at 24 hours post-infection showing that it is sensitive enough to detect the expression of S protein during infection.