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PMC:7376845 / 21939-24732 JSONTXT

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LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T143 60-67 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T144 106-113 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T145 165-170 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T146 295-302 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T147 340-350 Body_part denotes Antibodies http://purl.org/sig/ont/fma/fma62871
T148 383-390 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T149 423-430 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T150 464-471 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T151 499-504 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T152 520-527 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T153 612-620 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T154 706-713 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T155 721-728 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T156 851-859 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T157 937-941 Body_part denotes cell http://purl.org/sig/ont/fma/fma68646
T158 1011-1016 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T159 1053-1060 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T160 1255-1263 Body_part denotes proteins http://purl.org/sig/ont/fma/fma67257
T161 1443-1450 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T162 1532-1539 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T163 1668-1676 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T164 1851-1859 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T165 1876-1884 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T166 2012-2017 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T167 2035-2040 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T168 2077-2084 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T169 2194-2202 Body_part denotes antibody http://purl.org/sig/ont/fma/fma62871
T170 2212-2216 Body_part denotes Cell http://purl.org/sig/ont/fma/fma68646
T171 2333-2340 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T172 2463-2470 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T173 2606-2613 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257
T174 2746-2753 Body_part denotes protein http://purl.org/sig/ont/fma/fma67257

LitCovid-PD-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T6 2262-2267 Body_part denotes Scale http://purl.obolibrary.org/obo/UBERON_0002542

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
549 5-11 Species denotes murine Tax:10090
556 104-105 Gene denotes S Gene:43740568
557 293-294 Gene denotes S Gene:43740568
558 117-127 Species denotes SARS-CoV-2 Tax:2697049
559 306-316 Species denotes SARS-CoV-2 Tax:2697049
560 149-154 CellLine denotes 293FT CVCL:6911
561 159-164 CellLine denotes COS-7 CVCL:0224
563 370-380 Species denotes SARS-CoV-2 Tax:2697049
570 741-744 Gene denotes Myc Gene:4609
571 715-720 Gene denotes spike Gene:43740568
572 643-653 Species denotes SARS-CoV-2 Tax:2697049
573 655-702 Species denotes severe acute respiratory syndrome coronavirus 2 Tax:2697049
574 559-563 Chemical denotes DAPI MESH:C007293
575 565-594 Chemical denotes 4′,6-diamidino-2-phenylindole MESH:C007293
583 880-888 Gene denotes 2B2, 1A9 Gene:628883
584 1099-1100 Gene denotes S Gene:43740568
585 1040-1050 Species denotes SARS-CoV-2 Tax:2697049
586 1088-1098 Species denotes SARS-CoV-2 Tax:2697049
587 1141-1152 Species denotes horseradish Tax:3704
588 931-936 CellLine denotes 293FT CVCL:6911
589 1005-1010 CellLine denotes 293FT CVCL:6911
593 1373-1381 Gene denotes 2B2, 1A9 Gene:628883
594 1430-1440 Species denotes SARS-CoV-2 Tax:2697049
595 1519-1529 Species denotes SARS-CoV-2 Tax:2697049
602 1905-1908 Gene denotes Myc Gene:4609
603 1910-1918 Gene denotes 2B2, 1A9 Gene:628883
604 2064-2074 Species denotes SARS-CoV-2 Tax:2697049
605 2157-2172 Chemical denotes Alexa Fluor 488
606 2249-2253 Chemical denotes DAPI MESH:C007293
607 2006-2011 CellLine denotes COS-7 CVCL:0224
610 2604-2605 Gene denotes S Gene:43740568
611 2617-2627 Species denotes SARS-CoV-2 Tax:2697049

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T194 71-79 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T195 71-75 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T196 117-125 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T197 117-121 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T198 306-314 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T199 306-310 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T200 370-378 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T201 370-374 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T202 622-624 Disease denotes OD http://purl.obolibrary.org/obo/MONDO_0017178
T203 643-651 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T204 643-647 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T205 655-702 Disease denotes severe acute respiratory syndrome coronavirus 2 http://purl.obolibrary.org/obo/MONDO_0100096
T206 655-688 Disease denotes severe acute respiratory syndrome http://purl.obolibrary.org/obo/MONDO_0005091
T207 1040-1048 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T208 1040-1044 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T209 1088-1096 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T210 1088-1092 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T211 1430-1438 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T212 1430-1434 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T213 1519-1527 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T214 1519-1523 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T215 2064-2072 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T216 2064-2068 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091
T217 2617-2625 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T218 2617-2621 Disease denotes SARS http://purl.obolibrary.org/obo/MONDO_0005091

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T235 159-170 http://purl.obolibrary.org/obo/CLO_0051656 denotes COS-7 cells
T236 159-170 http://purl.obolibrary.org/obo/CLO_0051657 denotes COS-7 cells
T237 159-170 http://purl.obolibrary.org/obo/CLO_0051658 denotes COS-7 cells
T238 159-164 http://purl.obolibrary.org/obo/CLO_0002597 denotes COS-7
T239 159-164 http://purl.obolibrary.org/obo/CLO_0050508 denotes COS-7
T240 393-394 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T241 449-450 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T242 499-504 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T243 796-797 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T244 818-819 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T245 899-903 http://purl.obolibrary.org/obo/CLO_0001165 denotes 1G10
T246 937-941 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T247 968-976 http://purl.obolibrary.org/obo/CLO_0007225 denotes labelled
T248 1011-1016 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T249 1078-1086 http://purl.obolibrary.org/obo/CLO_0007225 denotes labelled
T250 1127-1135 http://purl.obolibrary.org/obo/CLO_0007225 denotes labelled
T251 1197-1198 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T252 1353-1354 http://purl.obolibrary.org/obo/CLO_0001021 denotes B
T253 1392-1396 http://purl.obolibrary.org/obo/CLO_0001165 denotes 1G10
T254 1595-1596 http://purl.obolibrary.org/obo/CLO_0001020 denotes A
T255 1929-1933 http://purl.obolibrary.org/obo/CLO_0001165 denotes 1G10
T256 2006-2017 http://purl.obolibrary.org/obo/CLO_0051656 denotes COS-7 cells
T257 2006-2017 http://purl.obolibrary.org/obo/CLO_0051657 denotes COS-7 cells
T258 2006-2017 http://purl.obolibrary.org/obo/CLO_0051658 denotes COS-7 cells
T259 2006-2011 http://purl.obolibrary.org/obo/CLO_0002597 denotes COS-7
T260 2006-2011 http://purl.obolibrary.org/obo/CLO_0050508 denotes COS-7
T261 2035-2040 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T262 2212-2216 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T263 2633-2639 http://purl.obolibrary.org/obo/UBERON_0000473 denotes tested

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T194 60-67 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T195 106-113 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T196 295-302 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T197 383-390 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T198 423-430 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T199 464-471 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T200 520-527 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T201 559-563 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T202 582-594 Chemical denotes phenylindole http://purl.obolibrary.org/obo/CHEBI_48559
T203 706-713 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T204 721-728 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T205 1053-1060 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T206 1255-1263 Chemical denotes proteins http://purl.obolibrary.org/obo/CHEBI_36080
T207 1443-1450 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T208 1532-1539 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T209 2077-2084 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T210 2157-2172 Chemical denotes Alexa Fluor 488 http://purl.obolibrary.org/obo/CHEBI_52661
T211 2163-2168 Chemical denotes Fluor http://purl.obolibrary.org/obo/CHEBI_24061
T212 2217-2223 Chemical denotes nuclei http://purl.obolibrary.org/obo/CHEBI_33252
T213 2249-2253 Chemical denotes DAPI http://purl.obolibrary.org/obo/CHEBI_51231
T214 2333-2340 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T215 2463-2470 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T216 2606-2613 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080
T217 2746-2753 Chemical denotes protein http://purl.obolibrary.org/obo/CHEBI_36080

LitCovid-PD-GlycoEpitope

Id Subject Object Predicate Lexical cue glyco_epitope_db_id
T4 899-903 GlycoEpitope denotes 1G10 http://www.glycoepitope.jp/epitopes/AN0438
T5 1392-1396 GlycoEpitope denotes 1G10 http://www.glycoepitope.jp/epitopes/AN0438
T6 1929-1933 GlycoEpitope denotes 1G10 http://www.glycoepitope.jp/epitopes/AN0438

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T167 0-81 Sentence denotes Four murine monoclonal antibodies bind to the full-length S protein of SARS-CoV-2
T168 82-231 Sentence denotes Next, the full-length S protein of SARS-CoV-2 was overexpressed in 293FT and COS-7 cells and detected with each of the mAbs using WB and IF analyses.
T169 232-329 Sentence denotes As shown in Figure 3, all four mAbs bound to the full-length S protein of SARS-CoV-2 (Figure 3A).
T170 330-558 Sentence denotes Figure 3 Antibodies expected to target SARS-CoV-2 S protein, (A) hybridise to the denatured protein in western blot, (B) bind to the protein in ELISA and (C) recognise cells expressing the protein as shown by immunofluorescence
T171 559-564 Sentence denotes DAPI:
T172 565-795 Sentence denotes 4′,6-diamidino-2-phenylindole; mAb: monoclonal antibody: OD: optical density; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; S protein: spike protein; vec: pXJ40-Myc expression vector used as an empty vector control.
T173 796-798 Sentence denotes A.
T174 799-905 Sentence denotes Each photo depicts a western blot using the primary antibody indicated below it (2B2, 1A9, 4B12, or 1G10).
T175 906-1102 Sentence denotes Empty vector-transfected 293FT cell products are on the lanes labelled as ‘Vec’, while products of 293FT cells expressing full-length SARS-CoV-2 S protein are on the lanes labelled ‘SARS-CoV-2 S’.
T176 1103-1196 Sentence denotes Primary antibodies were labelled with horseradish peroxidase-conjugated secondary antibodies.
T177 1197-1352 Sentence denotes A ladder indicative of the molecular weights in kD of the proteins relative to their vertical position on the blots, is indicated on the left of the panel.
T178 1353-1355 Sentence denotes B.
T179 1356-1475 Sentence denotes The abilities of 2B2, 1A9, 4B12 and 1G10 monoclonal antibodies to bind to SARS-CoV-2 S protein was determined by ELISA.
T180 1476-1594 Sentence denotes Individual wells were coated with 20 ng of SARS-CoV-2 S protein and incubated with serially diluted mAbs as indicated.
T181 1595-1776 Sentence denotes A representative plot from three independent experiment is show for each antibody and error bars correspond to standard deviations of each mAb experiment carried out in triplicates.
T182 1777-1779 Sentence denotes C.
T183 1780-1935 Sentence denotes Each photo depicts an immunofluorescence assay using either no primary antibody, or the primary antibody indicated below it (Myc, 2B2, 1A9, 4B12, or 1G10).
T184 1936-2101 Sentence denotes Immunofluorescence analysis was performed on empty vector-transfected COS-7 cells (top photos) and cells expressing full-length SARS-CoV-2 S protein (bottom photos).
T185 2102-2211 Sentence denotes The indicated primary antibodies were used followed by Alexa Fluor 488-conjugated secondary antibody (green).
T186 2212-2261 Sentence denotes Cell nuclei were counterstained with DAPI (blue).
T187 2262-2280 Sentence denotes Scale bar = 50 µm.
T188 2281-2449 Sentence denotes The binding of these mAbs to recombinant purified S protein was also determined using indirect ELISA where different concentrations of antibodies were used for binding.
T189 2450-2553 Sentence denotes Binding to S protein was observed for all four mAbs with 1A9 showing the strongest binding (Figure 3B).
T190 2554-2659 Sentence denotes Similarly, all four mAbs bound to the full-length S protein of SARS-CoV-2 when tested via IF (Figure 3C).
T191 2660-2793 Sentence denotes Collectively our data demonstrates the ability of all four mAbs to bind full-length S protein in both its native and denatured forms.