PMC:7376845 / 11231-11824 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T72","span":{"begin":239,"end":243},"obj":"Body_part"},{"id":"T73","span":{"begin":448,"end":453},"obj":"Body_part"},{"id":"T74","span":{"begin":520,"end":527},"obj":"Body_part"}],"attributes":[{"id":"A72","pred":"fma_id","subj":"T72","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A73","pred":"fma_id","subj":"T73","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A74","pred":"fma_id","subj":"T74","obj":"http://purl.org/sig/ont/fma/fma67257"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"LitCovid-PubTator","denotations":[{"id":"279","span":{"begin":4,"end":9},"obj":"Species"},{"id":"280","span":{"begin":14,"end":20},"obj":"Chemical"},{"id":"281","span":{"begin":444,"end":453},"obj":"CellLine"}],"attributes":[{"id":"A279","pred":"tao:has_database_id","subj":"279","obj":"Tax:9606"},{"id":"A281","pred":"tao:has_database_id","subj":"281","obj":"CVCL:0045"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T112","span":{"begin":165,"end":167},"obj":"Disease"}],"attributes":[{"id":"A112","pred":"mondo_id","subj":"T112","obj":"http://purl.obolibrary.org/obo/MONDO_0024555"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T131","span":{"begin":4,"end":9},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T132","span":{"begin":38,"end":39},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T133","span":{"begin":80,"end":82},"obj":"http://purl.obolibrary.org/obo/CLO_0050507"},{"id":"T134","span":{"begin":205,"end":210},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T135","span":{"begin":239,"end":243},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T136","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0001230"},{"id":"T137","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0037237"},{"id":"T138","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0050903"},{"id":"T139","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0054249"},{"id":"T140","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0054250"},{"id":"T141","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0054251"},{"id":"T142","span":{"begin":444,"end":447},"obj":"http://purl.obolibrary.org/obo/CLO_0054252"},{"id":"T143","span":{"begin":448,"end":453},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T108","span":{"begin":105,"end":107},"obj":"Chemical"},{"id":"T109","span":{"begin":165,"end":167},"obj":"Chemical"},{"id":"T110","span":{"begin":520,"end":527},"obj":"Chemical"}],"attributes":[{"id":"A108","pred":"chebi_id","subj":"T108","obj":"http://purl.obolibrary.org/obo/CHEBI_73700"},{"id":"A109","pred":"chebi_id","subj":"T109","obj":"http://purl.obolibrary.org/obo/CHEBI_75013"},{"id":"A110","pred":"chebi_id","subj":"T110","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T3","span":{"begin":244,"end":253},"obj":"http://purl.obolibrary.org/obo/GO_0009058"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"LitCovid-sentences","denotations":[{"id":"T79","span":{"begin":0,"end":84},"obj":"Sentence"},{"id":"T80","span":{"begin":85,"end":406},"obj":"Sentence"},{"id":"T81","span":{"begin":407,"end":593},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"2_test","denotations":[{"id":"32700671-16796401-29327477","span":{"begin":80,"end":82},"obj":"16796401"},{"id":"32700671-31906790-29327478","span":{"begin":589,"end":591},"obj":"31906790"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}

{"project":"MyTest","denotations":[{"id":"32700671-16796401-29327477","span":{"begin":80,"end":82},"obj":"16796401"},{"id":"32700671-31906790-29327478","span":{"begin":589,"end":591},"obj":"31906790"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"The human mAb CR3022 was expressed in a similar manner as previously described [22]. The variable heavy (VH; GenBank accession number: DQ168569) and variable light (VL; GenBank accession number: DQ168570) genes of CR3022 were generated by gene synthesis (Bio Basic Asia Pacific) and cloned into pFUSEss-CHIg-hIgG1 and pFUSE2ss-CLIg-hK cloning vectors (InvivoGen, San Diego, CA, United States) respectively. Transfection of suspension FreeStyle 293 cells (Thermo Fisher Scientific) and purification of antibodies by fast protein liquid chromatography is as described in our previous study [23]."}