PMC:7376845 / 10288-10522 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T69","span":{"begin":96,"end":101},"obj":"Body_part"}],"attributes":[{"id":"A69","pred":"fma_id","subj":"T69","obj":"http://purl.org/sig/ont/fma/fma63083"}],"text":"Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T4","span":{"begin":96,"end":101},"obj":"Body_part"}],"attributes":[{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour."}

{"project":"LitCovid-PubTator","denotations":[{"id":"270","span":{"begin":96,"end":109},"obj":"Gene"},{"id":"272","span":{"begin":139,"end":145},"obj":"Species"}],"attributes":[{"id":"A270","pred":"tao:has_database_id","subj":"270","obj":"Gene:11657"},{"id":"A272","pred":"tao:has_database_id","subj":"272","obj":"Tax:10090"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T104","span":{"begin":188,"end":194},"obj":"Chemical"}],"attributes":[{"id":"A104","pred":"chebi_id","subj":"T104","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"}],"text":"Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour."}

{"project":"LitCovid-sentences","denotations":[{"id":"T74","span":{"begin":0,"end":234},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Plates were then washed four times with 0.05% Tween 20 in PBS (PBST) and blocked with 5% bovine serum albumin (BSA)/PBST for 30 min before murine antibodies serially diluted with blocking buffer were added to desired wells for 1 hour."}