PMC:7366549 / 7343-7900 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T19","span":{"begin":43,"end":46},"obj":"Body_part"},{"id":"T20","span":{"begin":110,"end":113},"obj":"Body_part"},{"id":"T21","span":{"begin":114,"end":117},"obj":"Body_part"},{"id":"T22","span":{"begin":366,"end":369},"obj":"Body_part"},{"id":"T23","span":{"begin":380,"end":383},"obj":"Body_part"}],"attributes":[{"id":"A19","pred":"fma_id","subj":"T19","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A20","pred":"fma_id","subj":"T20","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A21","pred":"fma_id","subj":"T21","obj":"http://purl.org/sig/ont/fma/fma74412"},{"id":"A22","pred":"fma_id","subj":"T22","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A23","pred":"fma_id","subj":"T23","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"161","span":{"begin":13,"end":28},"obj":"Species"},{"id":"162","span":{"begin":233,"end":241},"obj":"Chemical"},{"id":"163","span":{"begin":463,"end":470},"obj":"Chemical"}],"attributes":[{"id":"A161","pred":"tao:has_database_id","subj":"161","obj":"Tax:1508220"},{"id":"A162","pred":"tao:has_database_id","subj":"162","obj":"MESH:D010984"},{"id":"A163","pred":"tao:has_database_id","subj":"163","obj":"MESH:D012685"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T99","span":{"begin":13,"end":16},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"},{"id":"T100","span":{"begin":70,"end":73},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9397"}],"text":"Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T6","span":{"begin":114,"end":117},"obj":"Chemical"},{"id":"T7","span":{"begin":463,"end":470},"obj":"Chemical"},{"id":"T8","span":{"begin":505,"end":507},"obj":"Chemical"}],"attributes":[{"id":"A6","pred":"chebi_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A7","pred":"chebi_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CHEBI_2511"},{"id":"A8","pred":"chebi_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CHEBI_73612"}],"text":"Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA)."}

{"project":"2_test","denotations":[{"id":"32317409-15681402-47001146","span":{"begin":406,"end":408},"obj":"15681402"},{"id":"T37699","span":{"begin":406,"end":408},"obj":"15681402"}],"text":"Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T55","span":{"begin":0,"end":150},"obj":"Sentence"},{"id":"T56","span":{"begin":151,"end":409},"obj":"Sentence"},{"id":"T57","span":{"begin":410,"end":557},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Detection of bat coronavirus using RT-PCR: RNA was extracted from the bat specimens using the MagMAX pathogen RNA/DNA isolation kit (Invitrogen, USA). RT-PCR was performed using Superscript III one-step RT-PCR (Invitrogen, USA) with Platinum High-Fidelity Taq polymerase (Invitrogen, USA) using the published BtCoV-specific primers targeting the conserved region of RNA-dependent RNA polymerase (RdRp) gene21. The amplicon of 440 bp was separated on 1.5 per cent agarose gel and visualized under VersaDoc MP 4000 ultraviolet transilluminator (Bio-Rad, USA)."}