PMC:7321036 / 99619-100349 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7321036","sourcedb":"PMC","sourceid":"7321036","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7321036","text":"(Institut Pasteur, Paris). Detection of viral genomes was performed by RT-qPCR, directly from the inactivated supernatant (Lista et al., 2020). SARS-CoV-2 specific primers targeting the N gene region: 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) (Chu et al., 2020) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler, with the following cycling conditions: 55°C for 10 minutes, 95°C for 1 minute, and 40 cycles of 95°C for 10 s, followed by 60°C for 1 minute. The number of viral genomes is expressed as PFU equivalents/mL, and was calculated by performing a standard curve with RNA derived from a viral stock with a known viral titer.","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T1","span":{"begin":46,"end":53},"obj":"Body_part"},{"id":"T2","span":{"begin":188,"end":192},"obj":"Body_part"},{"id":"T3","span":{"begin":575,"end":582},"obj":"Body_part"},{"id":"T4","span":{"begin":674,"end":677},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"fma_id","subj":"T1","obj":"http://purl.org/sig/ont/fma/fma84116"},{"id":"A2","pred":"fma_id","subj":"T2","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A3","pred":"fma_id","subj":"T3","obj":"http://purl.org/sig/ont/fma/fma84116"},{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma67095"},{"subj":"T1","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T2","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T385","span":{"begin":144,"end":152},"obj":"Disease"}],"attributes":[{"id":"A385","pred":"mondo_id","subj":"T385","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"subj":"T385","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1","span":{"begin":188,"end":192},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T2","span":{"begin":652,"end":653},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T3","span":{"begin":691,"end":692},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T4","span":{"begin":710,"end":711},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"attributes":[{"subj":"T1","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T2","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T3","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T4","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"2286","span":{"begin":186,"end":187},"obj":"Gene"},{"id":"2287","span":{"begin":144,"end":154},"obj":"Species"}],"attributes":[{"id":"A2286","pred":"tao:has_database_id","subj":"2286","obj":"Gene:43740575"},{"id":"A2287","pred":"tao:has_database_id","subj":"2287","obj":"Tax:2697049"},{"subj":"2286","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2287","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T870","span":{"begin":0,"end":26},"obj":"Sentence"},{"id":"T871","span":{"begin":27,"end":143},"obj":"Sentence"},{"id":"T872","span":{"begin":144,"end":200},"obj":"Sentence"},{"id":"T873","span":{"begin":201,"end":451},"obj":"Sentence"},{"id":"T874","span":{"begin":452,"end":554},"obj":"Sentence"},{"id":"T875","span":{"begin":555,"end":730},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T870","pred":"source","obj":"LitCovid-sentences"},{"subj":"T871","pred":"source","obj":"LitCovid-sentences"},{"subj":"T872","pred":"source","obj":"LitCovid-sentences"},{"subj":"T873","pred":"source","obj":"LitCovid-sentences"},{"subj":"T874","pred":"source","obj":"LitCovid-sentences"},{"subj":"T875","pred":"source","obj":"LitCovid-sentences"}]},{"project":"2_test","denotations":[{"id":"32645325-32225176-20773112","span":{"begin":137,"end":141},"obj":"32225176"},{"id":"32645325-32031583-20773113","span":{"begin":293,"end":297},"obj":"32031583"}],"attributes":[{"subj":"32645325-32225176-20773112","pred":"source","obj":"2_test"},{"subj":"32645325-32031583-20773113","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-FMA-UBERON","color":"#93ec94","default":true},{"id":"LitCovid-PD-MONDO","color":"#ec93ac"},{"id":"LitCovid-PD-CLO","color":"#93c6ec"},{"id":"LitCovid-PubTator","color":"#e0ec93"},{"id":"LitCovid-sentences","color":"#de93ec"},{"id":"2_test","color":"#93ecc4"}]}]}}