PMC:7321036 / 92801-93070 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T658","span":{"begin":9,"end":14},"obj":"Body_part"},{"id":"T659","span":{"begin":146,"end":150},"obj":"Body_part"}],"attributes":[{"id":"A658","pred":"fma_id","subj":"T658","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A659","pred":"fma_id","subj":"T659","obj":"http://purl.org/sig/ont/fma/fma68646"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T364","span":{"begin":204,"end":206},"obj":"Disease"}],"attributes":[{"id":"A364","pred":"mondo_id","subj":"T364","obj":"http://purl.obolibrary.org/obo/MONDO_0007191"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1260","span":{"begin":9,"end":14},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1261","span":{"begin":146,"end":150},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1262","span":{"begin":224,"end":225},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T551","span":{"begin":79,"end":91},"obj":"Chemical"},{"id":"T552","span":{"begin":93,"end":97},"obj":"Chemical"}],"attributes":[{"id":"A551","pred":"chebi_id","subj":"T551","obj":"http://purl.obolibrary.org/obo/CHEBI_48559"},{"id":"A552","pred":"chebi_id","subj":"T552","obj":"http://purl.obolibrary.org/obo/CHEBI_51231"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T467","span":{"begin":146,"end":156},"obj":"http://purl.obolibrary.org/obo/GO_0007049"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2076","span":{"begin":62,"end":91},"obj":"Chemical"},{"id":"2077","span":{"begin":93,"end":97},"obj":"Chemical"},{"id":"2078","span":{"begin":110,"end":113},"obj":"Chemical"}],"attributes":[{"id":"A2076","pred":"tao:has_database_id","subj":"2076","obj":"MESH:C007293"},{"id":"A2077","pred":"tao:has_database_id","subj":"2077","obj":"MESH:C007293"},{"id":"A2078","pred":"tao:has_database_id","subj":"2078","obj":"MESH:D007854"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}

{"project":"LitCovid-sentences","denotations":[{"id":"T815","span":{"begin":0,"end":269},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Finally, cells were pelleted again and incubated with 1 mg/mL 4′,6-diamidino-2-phenylindole (DAPI; Thermo) in PBS for 30 minutes before analyzing cell cycle using fluorescence by flow cytometry using the BD FACSymphony with a violet laser (405 nm) and 450/50 nm filter."}