PMC:7321036 / 90218-92291 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7321036","sourcedb":"PMC","sourceid":"7321036","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7321036","text":"The N-protein cassette was subcloned from pLVX-EF1a-SARS-CoV-2-N-2xStrep-IRES-Puro (Gordon et al., 2020) into pLVX-TetOne-Puro (Takara) using the restriction enzymes EcoRI and BamHI to create pLVX-TetOne-Puro-SARS-CoV-2-N-2xStrep. Sequence integrity was confirmed by Sanger sequencing (GeneWiz). To produce lentiviruses, HEK293T cells (50% confluent in a T175 flask) were transfected with 5 μg of each of the pLVX-TetOne-Puro lentiviral plasmids, 3.33 μg Gag-Pol packaging construct, and 1.66 μg VSV-G envelope (pMD2.G, Addgene) using PolyJet (SignaGen). Culture supernatant was precipitated with a final concentration of 8.5% PEG-6000 and 0.3M sodium chloride (NaCl), incubated at 4°C for 2-4 hours. Virions were pelleted at 3500 rpm for 20 minutes in a spin bucket rotor, suspended in 0.5 mL 1xPBS, and aliquoted for storage at 80°C. Vero E6 cells were seeded in T75 flasks at 50% confluence and transduced with 200 μL precipitated lentivirus derived from pLVX-TetOne-Puro-SARS-CoV-2-N-2xStrep or pLVX-TetOne-Puro empty vector. 48 hours post transduction, 10 μg/mL Puromycin was added to cultures to select transduced cells. Polyclonal stable cell lines were seeded into 15 cm dishes in triplicate and treated with 1 μg/mL doxycycline for 48 hours. Cells were washed in ice cold PBS and harvested by scraping in cold PBS. Cell pellets were lysed directly (8 M urea, 100 mM ammonium bicarbonate (ABC), 150 mM NaCl, protease inhibitor (mini-cOmplete, Roche) and phosphatase inhibitors (phosSTOP, Roche), and protein digestion and phosphopeptide enrichment was performed as described above for SARS-CoV-2 infected Vero E6 cells. During the analysis, one of three replicates of the N-overexpressed samples was found to be an outlier based on principal component analysis (as in Figures S1A and S1B) and was removed. N-overexpression (n = 2) was compared to empty vector (EV) controls (n = 3) during analysis (full data available in Table S1). Kinase activity predictions were also performed the same as for viral infection phosphoproteomics (full data available in Table S4).","tracks":[{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T191","span":{"begin":367,"end":379},"obj":"SO:0000704"},{"id":"T192","span":{"begin":997,"end":1165},"obj":"GO:0065007"}],"attributes":[{"subj":"T191","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T192","pred":"source","obj":"LitCovid-PMC-OGER-BB"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T642","span":{"begin":6,"end":13},"obj":"Body_part"},{"id":"T643","span":{"begin":329,"end":334},"obj":"Body_part"},{"id":"T644","span":{"begin":455,"end":458},"obj":"Body_part"},{"id":"T645","span":{"begin":844,"end":849},"obj":"Body_part"},{"id":"T646","span":{"begin":1120,"end":1125},"obj":"Body_part"},{"id":"T647","span":{"begin":1145,"end":1149},"obj":"Body_part"},{"id":"T648","span":{"begin":1251,"end":1256},"obj":"Body_part"},{"id":"T649","span":{"begin":1324,"end":1328},"obj":"Body_part"},{"id":"T650","span":{"begin":1508,"end":1515},"obj":"Body_part"},{"id":"T651","span":{"begin":1621,"end":1626},"obj":"Body_part"}],"attributes":[{"id":"A642","pred":"fma_id","subj":"T642","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A643","pred":"fma_id","subj":"T643","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A644","pred":"fma_id","subj":"T644","obj":"http://purl.org/sig/ont/fma/fma63011"},{"id":"A645","pred":"fma_id","subj":"T645","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A646","pred":"fma_id","subj":"T646","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A647","pred":"fma_id","subj":"T647","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A648","pred":"fma_id","subj":"T648","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A649","pred":"fma_id","subj":"T649","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A650","pred":"fma_id","subj":"T650","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A651","pred":"fma_id","subj":"T651","obj":"http://purl.org/sig/ont/fma/fma68646"},{"subj":"T642","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T643","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T644","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T645","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T646","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T647","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T648","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T649","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T650","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T651","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T356","span":{"begin":52,"end":60},"obj":"Disease"},{"id":"T357","span":{"begin":209,"end":217},"obj":"Disease"},{"id":"T358","span":{"begin":975,"end":983},"obj":"Disease"},{"id":"T359","span":{"begin":1593,"end":1601},"obj":"Disease"},{"id":"T360","span":{"begin":1869,"end":1871},"obj":"Disease"},{"id":"T361","span":{"begin":2005,"end":2020},"obj":"Disease"},{"id":"T362","span":{"begin":2011,"end":2020},"obj":"Disease"}],"attributes":[{"id":"A356","pred":"mondo_id","subj":"T356","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A357","pred":"mondo_id","subj":"T357","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A358","pred":"mondo_id","subj":"T358","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A359","pred":"mondo_id","subj":"T359","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A360","pred":"mondo_id","subj":"T360","obj":"http://purl.obolibrary.org/obo/MONDO_0009176"},{"id":"A361","pred":"mondo_id","subj":"T361","obj":"http://purl.obolibrary.org/obo/MONDO_0005108"},{"id":"A362","pred":"mondo_id","subj":"T362","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"},{"subj":"T356","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T357","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T358","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T359","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T360","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T361","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T362","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1239","span":{"begin":329,"end":334},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1240","span":{"begin":353,"end":354},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1241","span":{"begin":597,"end":598},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1242","span":{"begin":682,"end":685},"obj":"http://purl.obolibrary.org/obo/CLO_0001387"},{"id":"T1243","span":{"begin":753,"end":754},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T1244","span":{"begin":836,"end":849},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T1245","span":{"begin":1030,"end":1032},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T1246","span":{"begin":1120,"end":1125},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1247","span":{"begin":1145,"end":1155},"obj":"http://purl.obolibrary.org/obo/CLO_0000031"},{"id":"T1248","span":{"begin":1241,"end":1243},"obj":"http://purl.obolibrary.org/obo/CLO_0001382"},{"id":"T1249","span":{"begin":1251,"end":1256},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1250","span":{"begin":1324,"end":1328},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1251","span":{"begin":1613,"end":1626},"obj":"http://purl.obolibrary.org/obo/CLO_0051719"},{"id":"T1252","span":{"begin":1936,"end":1938},"obj":"http://purl.obolibrary.org/obo/CLO_0050050"},{"id":"T1253","span":{"begin":1948,"end":1956},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"}],"attributes":[{"subj":"T1239","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1240","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1241","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1242","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1243","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1244","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1245","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1246","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1247","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1248","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1249","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1250","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1251","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1252","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T1253","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T529","span":{"begin":6,"end":13},"obj":"Chemical"},{"id":"T530","span":{"begin":627,"end":630},"obj":"Chemical"},{"id":"T531","span":{"begin":645,"end":660},"obj":"Chemical"},{"id":"T532","span":{"begin":645,"end":651},"obj":"Chemical"},{"id":"T533","span":{"begin":652,"end":660},"obj":"Chemical"},{"id":"T534","span":{"begin":662,"end":666},"obj":"Chemical"},{"id":"T535","span":{"begin":1067,"end":1076},"obj":"Chemical"},{"id":"T536","span":{"begin":1225,"end":1236},"obj":"Chemical"},{"id":"T538","span":{"begin":1362,"end":1366},"obj":"Chemical"},{"id":"T539","span":{"begin":1375,"end":1383},"obj":"Chemical"},{"id":"T540","span":{"begin":1397,"end":1400},"obj":"Chemical"},{"id":"T541","span":{"begin":1410,"end":1414},"obj":"Chemical"},{"id":"T542","span":{"begin":1416,"end":1434},"obj":"Chemical"},{"id":"T544","span":{"begin":1425,"end":1434},"obj":"Chemical"},{"id":"T545","span":{"begin":1474,"end":1484},"obj":"Chemical"},{"id":"T546","span":{"begin":1508,"end":1515},"obj":"Chemical"},{"id":"T547","span":{"begin":2069,"end":2071},"obj":"Chemical"}],"attributes":[{"id":"A529","pred":"chebi_id","subj":"T529","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A530","pred":"chebi_id","subj":"T530","obj":"http://purl.obolibrary.org/obo/CHEBI_46793"},{"id":"A531","pred":"chebi_id","subj":"T531","obj":"http://purl.obolibrary.org/obo/CHEBI_26710"},{"id":"A532","pred":"chebi_id","subj":"T532","obj":"http://purl.obolibrary.org/obo/CHEBI_26708"},{"id":"A533","pred":"chebi_id","subj":"T533","obj":"http://purl.obolibrary.org/obo/CHEBI_17996"},{"id":"A534","pred":"chebi_id","subj":"T534","obj":"http://purl.obolibrary.org/obo/CHEBI_26710"},{"id":"A535","pred":"chebi_id","subj":"T535","obj":"http://purl.obolibrary.org/obo/CHEBI_17939"},{"id":"A536","pred":"chebi_id","subj":"T536","obj":"http://purl.obolibrary.org/obo/CHEBI_50845"},{"id":"A537","pred":"chebi_id","subj":"T536","obj":"http://purl.obolibrary.org/obo/CHEBI_60648"},{"id":"A538","pred":"chebi_id","subj":"T538","obj":"http://purl.obolibrary.org/obo/CHEBI_16199"},{"id":"A539","pred":"chebi_id","subj":"T539","obj":"http://purl.obolibrary.org/obo/CHEBI_28938"},{"id":"A540","pred":"chebi_id","subj":"T540","obj":"http://purl.obolibrary.org/obo/CHEBI_421707"},{"id":"A541","pred":"chebi_id","subj":"T541","obj":"http://purl.obolibrary.org/obo/CHEBI_26710"},{"id":"A542","pred":"chebi_id","subj":"T542","obj":"http://purl.obolibrary.org/obo/CHEBI_37670"},{"id":"A543","pred":"chebi_id","subj":"T542","obj":"http://purl.obolibrary.org/obo/CHEBI_60258"},{"id":"A544","pred":"chebi_id","subj":"T544","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A545","pred":"chebi_id","subj":"T545","obj":"http://purl.obolibrary.org/obo/CHEBI_35222"},{"id":"A546","pred":"chebi_id","subj":"T546","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A547","pred":"chebi_id","subj":"T547","obj":"http://purl.obolibrary.org/obo/CHEBI_29401"},{"subj":"T529","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T530","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T531","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T532","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T533","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T534","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T535","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T536","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T538","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T539","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T540","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T541","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T542","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T544","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T545","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T546","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T547","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T459","span":{"begin":819,"end":826},"obj":"http://purl.obolibrary.org/obo/GO_0051235"},{"id":"T460","span":{"begin":1044,"end":1056},"obj":"http://purl.obolibrary.org/obo/GO_0009293"},{"id":"T461","span":{"begin":1462,"end":1473},"obj":"http://purl.obolibrary.org/obo/GO_0016791"},{"id":"T462","span":{"begin":1508,"end":1525},"obj":"http://purl.obolibrary.org/obo/GO_0044256"},{"id":"T463","span":{"begin":1516,"end":1525},"obj":"http://purl.obolibrary.org/obo/GO_0007586"},{"id":"T464","span":{"begin":1941,"end":1956},"obj":"http://purl.obolibrary.org/obo/GO_0016301"},{"id":"T465","span":{"begin":2005,"end":2020},"obj":"http://purl.obolibrary.org/obo/GO_0016032"}],"attributes":[{"subj":"T459","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T460","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T461","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T462","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T463","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T464","pred":"source","obj":"LitCovid-PD-GO-BP"},{"subj":"T465","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"2029","span":{"begin":47,"end":51},"obj":"Gene"},{"id":"2030","span":{"begin":1360,"end":1361},"obj":"Gene"},{"id":"2031","span":{"begin":986,"end":987},"obj":"Gene"},{"id":"2032","span":{"begin":220,"end":221},"obj":"Gene"},{"id":"2033","span":{"begin":63,"end":64},"obj":"Gene"},{"id":"2034","span":{"begin":4,"end":5},"obj":"Gene"},{"id":"2035","span":{"begin":52,"end":62},"obj":"Species"},{"id":"2036","span":{"begin":209,"end":219},"obj":"Species"},{"id":"2037","span":{"begin":975,"end":985},"obj":"Species"},{"id":"2038","span":{"begin":1593,"end":1603},"obj":"Species"},{"id":"2039","span":{"begin":496,"end":499},"obj":"Species"},{"id":"2040","span":{"begin":115,"end":121},"obj":"Chemical"},{"id":"2041","span":{"begin":197,"end":203},"obj":"Chemical"},{"id":"2042","span":{"begin":627,"end":635},"obj":"Chemical"},{"id":"2043","span":{"begin":645,"end":660},"obj":"Chemical"},{"id":"2044","span":{"begin":662,"end":666},"obj":"Chemical"},{"id":"2045","span":{"begin":963,"end":969},"obj":"Chemical"},{"id":"2046","span":{"begin":1004,"end":1010},"obj":"Chemical"},{"id":"2047","span":{"begin":1067,"end":1076},"obj":"Chemical"},{"id":"2048","span":{"begin":1225,"end":1236},"obj":"Chemical"},{"id":"2049","span":{"begin":1362,"end":1366},"obj":"Chemical"},{"id":"2050","span":{"begin":1375,"end":1395},"obj":"Chemical"},{"id":"2051","span":{"begin":1410,"end":1414},"obj":"Chemical"},{"id":"2052","span":{"begin":1276,"end":1284},"obj":"Disease"},{"id":"2053","span":{"begin":1314,"end":1322},"obj":"Disease"},{"id":"2054","span":{"begin":321,"end":328},"obj":"CellLine"},{"id":"2055","span":{"begin":841,"end":843},"obj":"CellLine"}],"attributes":[{"id":"A2029","pred":"tao:has_database_id","subj":"2029","obj":"Gene:1917"},{"id":"A2030","pred":"tao:has_database_id","subj":"2030","obj":"Gene:43740571"},{"id":"A2031","pred":"tao:has_database_id","subj":"2031","obj":"Gene:43740575"},{"id":"A2032","pred":"tao:has_database_id","subj":"2032","obj":"Gene:43740575"},{"id":"A2033","pred":"tao:has_database_id","subj":"2033","obj":"Gene:43740575"},{"id":"A2034","pred":"tao:has_database_id","subj":"2034","obj":"Gene:43740575"},{"id":"A2035","pred":"tao:has_database_id","subj":"2035","obj":"Tax:2697049"},{"id":"A2036","pred":"tao:has_database_id","subj":"2036","obj":"Tax:2697049"},{"id":"A2037","pred":"tao:has_database_id","subj":"2037","obj":"Tax:2697049"},{"id":"A2038","pred":"tao:has_database_id","subj":"2038","obj":"Tax:2697049"},{"id":"A2039","pred":"tao:has_database_id","subj":"2039","obj":"Tax:11276"},{"id":"A2042","pred":"tao:has_database_id","subj":"2042","obj":"MESH:C000595215"},{"id":"A2043","pred":"tao:has_database_id","subj":"2043","obj":"MESH:D012965"},{"id":"A2044","pred":"tao:has_database_id","subj":"2044","obj":"MESH:D012965"},{"id":"A2047","pred":"tao:has_database_id","subj":"2047","obj":"MESH:D011691"},{"id":"A2048","pred":"tao:has_database_id","subj":"2048","obj":"MESH:D004318"},{"id":"A2049","pred":"tao:has_database_id","subj":"2049","obj":"MESH:D014508"},{"id":"A2050","pred":"tao:has_database_id","subj":"2050","obj":"MESH:C027043"},{"id":"A2051","pred":"tao:has_database_id","subj":"2051","obj":"MESH:D012965"},{"id":"A2052","pred":"tao:has_database_id","subj":"2052","obj":"MESH:D011535"},{"id":"A2053","pred":"tao:has_database_id","subj":"2053","obj":"MESH:D011535"},{"id":"A2054","pred":"tao:has_database_id","subj":"2054","obj":"CVCL:0063"},{"id":"A2055","pred":"tao:has_database_id","subj":"2055","obj":"CVCL:4582"},{"subj":"2029","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2030","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2031","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2032","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2033","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2034","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2035","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2036","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2037","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2038","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2039","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2040","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2041","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2042","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2043","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2044","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2045","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2046","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2047","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2048","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2049","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2050","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2051","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2052","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2053","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2054","pred":"source","obj":"LitCovid-PubTator"},{"subj":"2055","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T796","span":{"begin":0,"end":230},"obj":"Sentence"},{"id":"T797","span":{"begin":231,"end":295},"obj":"Sentence"},{"id":"T798","span":{"begin":296,"end":554},"obj":"Sentence"},{"id":"T799","span":{"begin":555,"end":700},"obj":"Sentence"},{"id":"T800","span":{"begin":701,"end":835},"obj":"Sentence"},{"id":"T801","span":{"begin":836,"end":1029},"obj":"Sentence"},{"id":"T802","span":{"begin":1030,"end":1126},"obj":"Sentence"},{"id":"T803","span":{"begin":1127,"end":1250},"obj":"Sentence"},{"id":"T804","span":{"begin":1251,"end":1323},"obj":"Sentence"},{"id":"T805","span":{"begin":1324,"end":1627},"obj":"Sentence"},{"id":"T806","span":{"begin":1628,"end":1813},"obj":"Sentence"},{"id":"T807","span":{"begin":1814,"end":1940},"obj":"Sentence"},{"id":"T808","span":{"begin":1941,"end":2073},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T796","pred":"source","obj":"LitCovid-sentences"},{"subj":"T797","pred":"source","obj":"LitCovid-sentences"},{"subj":"T798","pred":"source","obj":"LitCovid-sentences"},{"subj":"T799","pred":"source","obj":"LitCovid-sentences"},{"subj":"T800","pred":"source","obj":"LitCovid-sentences"},{"subj":"T801","pred":"source","obj":"LitCovid-sentences"},{"subj":"T802","pred":"source","obj":"LitCovid-sentences"},{"subj":"T803","pred":"source","obj":"LitCovid-sentences"},{"subj":"T804","pred":"source","obj":"LitCovid-sentences"},{"subj":"T805","pred":"source","obj":"LitCovid-sentences"},{"subj":"T806","pred":"source","obj":"LitCovid-sentences"},{"subj":"T807","pred":"source","obj":"LitCovid-sentences"},{"subj":"T808","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PMC-OGER-BB","color":"#ec93dd","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#93ece1"},{"id":"LitCovid-PD-MONDO","color":"#ecc793"},{"id":"LitCovid-PD-CLO","color":"#ad93ec"},{"id":"LitCovid-PD-CHEBI","color":"#93ec93"},{"id":"LitCovid-PD-GO-BP","color":"#ec93ac"},{"id":"LitCovid-PubTator","color":"#93c7ec"},{"id":"LitCovid-sentences","color":"#e1ec93"}]}]}}