PMC:7321036 / 86812-87068 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T628","span":{"begin":27,"end":32},"obj":"Body_part"},{"id":"T629","span":{"begin":221,"end":225},"obj":"Body_part"},{"id":"T630","span":{"begin":226,"end":231},"obj":"Body_part"}],"attributes":[{"id":"A628","pred":"fma_id","subj":"T628","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A629","pred":"fma_id","subj":"T629","obj":"http://purl.org/sig/ont/fma/fma24984"},{"id":"A630","pred":"fma_id","subj":"T630","obj":"http://purl.org/sig/ont/fma/fma63083"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T27","span":{"begin":226,"end":231},"obj":"Body_part"}],"attributes":[{"id":"A27","pred":"uberon_id","subj":"T27","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T351","span":{"begin":17,"end":26},"obj":"Disease"}],"attributes":[{"id":"A351","pred":"mondo_id","subj":"T351","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1203","span":{"begin":27,"end":32},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T1204","span":{"begin":221,"end":225},"obj":"http://www.ebi.ac.uk/efo/EFO_0003051"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T470","span":{"begin":155,"end":167},"obj":"Chemical"}],"attributes":[{"id":"A470","pred":"chebi_id","subj":"T470","obj":"http://purl.obolibrary.org/obo/CHEBI_9750"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1936","span":{"begin":50,"end":53},"obj":"Chemical"},{"id":"1937","span":{"begin":70,"end":86},"obj":"Chemical"},{"id":"1938","span":{"begin":90,"end":93},"obj":"Chemical"},{"id":"1939","span":{"begin":155,"end":167},"obj":"Chemical"},{"id":"1940","span":{"begin":171,"end":174},"obj":"Chemical"},{"id":"1941","span":{"begin":235,"end":238},"obj":"Chemical"},{"id":"1947","span":{"begin":17,"end":26},"obj":"Disease"}],"attributes":[{"id":"A1936","pred":"tao:has_database_id","subj":"1936","obj":"MESH:D007854"},{"id":"A1937","pred":"tao:has_database_id","subj":"1937","obj":"MESH:C003043"},{"id":"A1938","pred":"tao:has_database_id","subj":"1938","obj":"MESH:D007854"},{"id":"A1939","pred":"tao:has_database_id","subj":"1939","obj":"MESH:D017830"},{"id":"A1940","pred":"tao:has_database_id","subj":"1940","obj":"MESH:D007854"},{"id":"A1941","pred":"tao:has_database_id","subj":"1941","obj":"MESH:D007854"},{"id":"A1947","pred":"tao:has_database_id","subj":"1947","obj":"MESH:D007239"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}

{"project":"LitCovid-sentences","denotations":[{"id":"T777","span":{"begin":0,"end":256},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"At 24 hours post-infection cells were washed with PBS and fixed in 4% paraformaldehyde in PBS for 20 minutes at RT, followed by permeabilization with 0.3% Triton X-100 in PBS for 10 minutes at RT and blocking in 5% fetal calf serum in PBS for 1 hour at RT."}