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PMC:7291971 / 85467-86967 JSONTXT

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LitCovid-PMC-OGER-BB

Id Subject Object Predicate Lexical cue
T1693 77-79 CHEBI:29238;CHEBI:29238 denotes 3H
T1694 240-247 CHEBI:60004;CHEBI:60004 denotes mixture
T1695 255-259 CHEBI:9754;CHEBI:9754 denotes Tris
T1696 260-263 CHEBI:17883;CHEBI:17883 denotes HCl
T1697 279-282 CHEBI:18320;CHEBI:18320 denotes DTT
T1698 289-294 CHEBI:6636;CHEBI:6636 denotes MgCl2
T1699 316-318 CHEBI:29238;CHEBI:29238 denotes 3H
T1700 390-398 SP_10 denotes SARS-CoV
T1701 399-404 PR:000000125 denotes nsp14
T1702 414-422 NCBITaxon:7742 denotes vaccinia
T1703 423-428 NCBITaxon:10239 denotes virus
T1704 464-469 SP_6;NCBITaxon:9606 denotes human
T1705 484-489 PR:O43148 denotes hRNMT
T1706 534-543 CHEBI:36357;CHEBI:36357 denotes compounds
T1707 562-566 CHEBI:51231;CHEBI:51231 denotes DMSO
T1708 577-581 CHEBI:51231;CHEBI:51231 denotes DMSO
T1709 712-716 CHEBI:51231;CHEBI:51231 denotes DMSO
T1710 728-736 CHEBI:60004;CHEBI:60004 denotes mixtures
T1711 801-806 CHEBI:15377;CHEBI:15377 denotes water
T1712 836-853 CHEBI:35702;CHEBI:35702 denotes diethylaminoethyl
T1713 988-1004 CHEBI:63050;CHEBI:63050 denotes ammonium formate
T1714 1024-1029 CHEBI:15377;CHEBI:15377 denotes water
T1715 1044-1051 CHEBI:16236;CHEBI:16236 denotes ethanol
T1716 1114-1116 CHEBI:29238;CHEBI:29238 denotes 3H
T1717 1432-1440 CHEBI:36357;CHEBI:36357 denotes compound
T18522 77-79 CHEBI:29238;CHEBI:29238 denotes 3H
T89446 240-247 CHEBI:60004;CHEBI:60004 denotes mixture
T7568 255-259 CHEBI:9754;CHEBI:9754 denotes Tris
T90528 260-263 CHEBI:17883;CHEBI:17883 denotes HCl
T32618 279-282 CHEBI:18320;CHEBI:18320 denotes DTT
T80620 289-294 CHEBI:6636;CHEBI:6636 denotes MgCl2
T65164 316-318 CHEBI:29238;CHEBI:29238 denotes 3H
T99852 390-398 SP_10 denotes SARS-CoV
T89465 399-404 PR:000000125 denotes nsp14
T92733 414-422 NCBITaxon:7742 denotes vaccinia
T32742 423-428 NCBITaxon:10239 denotes virus
T40449 464-469 SP_6;NCBITaxon:9606 denotes human
T9798 484-489 PR:O43148 denotes hRNMT
T22704 534-543 CHEBI:36357;CHEBI:36357 denotes compounds
T3161 562-566 CHEBI:51231;CHEBI:51231 denotes DMSO
T66008 577-581 CHEBI:51231;CHEBI:51231 denotes DMSO
T46228 712-716 CHEBI:51231;CHEBI:51231 denotes DMSO
T24685 728-736 CHEBI:60004;CHEBI:60004 denotes mixtures
T53961 801-806 CHEBI:15377;CHEBI:15377 denotes water
T66324 836-853 CHEBI:35702;CHEBI:35702 denotes diethylaminoethyl
T70177 988-1004 CHEBI:63050;CHEBI:63050 denotes ammonium formate
T31019 1024-1029 CHEBI:15377;CHEBI:15377 denotes water
T60694 1044-1051 CHEBI:16236;CHEBI:16236 denotes ethanol
T18203 1114-1116 CHEBI:29238;CHEBI:29238 denotes 3H
T42093 1432-1440 CHEBI:36357;CHEBI:36357 denotes compound

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T107 90-93 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T108 382-385 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T109 470-473 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T110 606-609 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T111 940-943 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T112 1124-1143 Body_part denotes transfer to the RNA http://purl.org/sig/ont/fma/fma67127
T113 1140-1143 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
1618 77-79 Chemical denotes 3H MESH:D014316
1619 255-259 Chemical denotes Tris
1620 260-263 Chemical denotes HCl MESH:D006851
1621 279-282 Chemical denotes DTT MESH:D004229
1622 289-294 Chemical denotes MgCl2 MESH:D015636
1623 316-318 Chemical denotes 3H MESH:D014316
1624 562-566 Chemical denotes DMSO MESH:D004121
1625 577-581 Chemical denotes DMSO MESH:D004121
1626 712-716 Chemical denotes DMSO MESH:D004121
1627 801-806 Chemical denotes water MESH:D014867
1628 836-853 Chemical denotes diethylaminoethyl
1629 855-859 Chemical denotes DEAE
1630 988-1004 Chemical denotes ammonium formate MESH:C030544
1631 1024-1029 Chemical denotes water MESH:D014867
1632 1044-1051 Chemical denotes ethanol MESH:D000431
1633 1114-1116 Chemical denotes 3H MESH:D014316

LitCovid-PD-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T102 390-398 Disease denotes SARS-CoV http://purl.obolibrary.org/obo/MONDO_0005091
T103 414-422 Disease denotes vaccinia http://purl.obolibrary.org/obo/MONDO_0002595

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T445 423-428 http://purl.obolibrary.org/obo/NCBITaxon_10239 denotes virus
T446 454-456 http://purl.obolibrary.org/obo/CLO_0053794 denotes 41
T447 464-469 http://purl.obolibrary.org/obo/NCBITaxon_9606 denotes human
T448 891-892 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T449 922-933 http://purl.obolibrary.org/obo/OBI_0000968 denotes Instruments
T450 1176-1177 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T451 1491-1499 http://purl.obolibrary.org/obo/CLO_0001658 denotes activity

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T950 64-70 Chemical denotes methyl http://purl.obolibrary.org/obo/CHEBI_32875|http://purl.obolibrary.org/obo/CHEBI_29309
T952 81-84 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T954 240-247 Chemical denotes mixture http://purl.obolibrary.org/obo/CHEBI_60004
T955 255-259 Chemical denotes Tris http://purl.obolibrary.org/obo/CHEBI_9754
T956 260-263 Chemical denotes HCl http://purl.obolibrary.org/obo/CHEBI_17883
T957 279-282 Chemical denotes DTT http://purl.obolibrary.org/obo/CHEBI_18320
T958 289-294 Chemical denotes MgCl2 http://purl.obolibrary.org/obo/CHEBI_6636
T959 301-304 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T961 319-322 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T963 562-566 Chemical denotes DMSO http://purl.obolibrary.org/obo/CHEBI_28262
T964 577-581 Chemical denotes DMSO http://purl.obolibrary.org/obo/CHEBI_28262
T965 624-627 Chemical denotes SAM http://purl.obolibrary.org/obo/CHEBI_15414|http://purl.obolibrary.org/obo/CHEBI_67040
T967 712-716 Chemical denotes DMSO http://purl.obolibrary.org/obo/CHEBI_28262
T968 801-806 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T969 855-859 Chemical denotes DEAE http://purl.obolibrary.org/obo/CHEBI_52153
T970 988-1004 Chemical denotes ammonium formate http://purl.obolibrary.org/obo/CHEBI_63050
T971 988-996 Chemical denotes ammonium http://purl.obolibrary.org/obo/CHEBI_28938
T972 997-1004 Chemical denotes formate http://purl.obolibrary.org/obo/CHEBI_15740|http://purl.obolibrary.org/obo/CHEBI_52343
T974 1024-1029 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377
T975 1044-1051 Chemical denotes ethanol http://purl.obolibrary.org/obo/CHEBI_16236
T976 1117-1123 Chemical denotes methyl http://purl.obolibrary.org/obo/CHEBI_32875|http://purl.obolibrary.org/obo/CHEBI_29309
T978 1223-1230 Chemical denotes Counter http://purl.obolibrary.org/obo/CHEBI_38960

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T51 1124-1143 http://purl.obolibrary.org/obo/GO_0030533 denotes transfer to the RNA

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T562 0-37 Sentence denotes 4.3 MTase filter binding assay (FBA)
T563 38-203 Sentence denotes The transfer of tritiated methyl from [3H] SAM onto RNA substrate was monitored by filter binding assay, performed according to the method described previously [40].
T564 204-499 Sentence denotes Assays were carried out in reaction mixture [40 mM Tris-HCl (pH 8.0), 1 mM DTT, 1 mM MgCl2, 2 μM SAM and 0.1 μM 3H-SAM (PerkinElmer)] in the presence of 0.7 μM GpppAC4 synthetic RNA and SARS-CoV nsp14 (50 nM), vaccinia virus capping enzyme (D1-D12) (41 U), or human RNA N7 MTase (hRNMT) (50 nM).
T565 500-656 Sentence denotes The enzymes were first mixed with compounds suspended in 100% DMSO (5% final DMSO) before the addition of RNA substrate and SAM and then incubated at 30 °C.
T566 657-717 Sentence denotes Control reactions were performed in the presence of 5% DMSO.
T567 718-807 Sentence denotes Reactions mixtures were stopped after 30 min by their 10-fold dilution in ice-cold water.
T568 808-935 Sentence denotes Samples were transferred to diethylaminoethyl (DEAE) filtermat (PerkinElmer) using a Filtermat Harvester (Packard Instruments).
T569 936-1052 Sentence denotes The RNA-retaining mats were washed twice with 10 mM ammonium formate pH 8.0, twice with water and once with ethanol.
T570 1053-1245 Sentence denotes They were soaked with scintillation fluid (PerkinElmer), and 3H-methyl transfer to the RNA substrates was determined using a Wallac MicroBeta TriLux Liquid Scintillation Counter (PerkinElmer).
T571 1246-1397 Sentence denotes For IC50 measurements, values were normalized and fitted with Prism (GraphPad software) using the following equation: Y = 100/(1+((X/IC50)^Hillslope)).
T572 1398-1500 Sentence denotes IC50 is defined as the inhibitory compound concentration that causes 50% reduction in enzyme activity.