PMC:7291971 / 85080-85465
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7291971","sourcedb":"PMC","sourceid":"7291971","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7291971","text":"SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).","tracks":[{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T1684","span":{"begin":0,"end":8},"obj":"SP_10"},{"id":"T1685","span":{"begin":9,"end":14},"obj":"PR:000000125"},{"id":"T1686","span":{"begin":19,"end":24},"obj":"SP_6;NCBITaxon:9606"},{"id":"T1687","span":{"begin":146,"end":154},"obj":"SO:0000155"},{"id":"T1688","span":{"begin":174,"end":183},"obj":"GO:0010467"},{"id":"T1689","span":{"begin":187,"end":194},"obj":"NCBITaxon:562"},{"id":"T1690","span":{"begin":258,"end":266},"obj":"NCBITaxon:7742"},{"id":"T1691","span":{"begin":267,"end":272},"obj":"NCBITaxon:10239"},{"id":"T1692","span":{"begin":296,"end":303},"obj":"GO:0032991"},{"id":"T42","span":{"begin":292,"end":317},"obj":"NCBITaxon:10239"},{"id":"T24440","span":{"begin":0,"end":8},"obj":"SP_10"},{"id":"T39744","span":{"begin":9,"end":14},"obj":"PR:000000125"},{"id":"T11149","span":{"begin":19,"end":24},"obj":"SP_6;NCBITaxon:9606"},{"id":"T17814","span":{"begin":146,"end":154},"obj":"SO:0000155"},{"id":"T90318","span":{"begin":174,"end":183},"obj":"GO:0010467"},{"id":"T4563","span":{"begin":187,"end":194},"obj":"NCBITaxon:562"},{"id":"T38822","span":{"begin":258,"end":266},"obj":"NCBITaxon:7742"},{"id":"T96822","span":{"begin":267,"end":272},"obj":"NCBITaxon:10239"},{"id":"T77113","span":{"begin":296,"end":303},"obj":"GO:0032991"}],"attributes":[{"subj":"T1684","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1685","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1686","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1687","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1688","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1689","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1690","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1691","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T1692","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T42","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T24440","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T39744","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T11149","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T17814","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T90318","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T4563","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T38822","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T96822","pred":"source","obj":"LitCovid-PMC-OGER-BB"},{"subj":"T77113","pred":"source","obj":"LitCovid-PMC-OGER-BB"}]},{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T103","span":{"begin":25,"end":28},"obj":"Body_part"},{"id":"T104","span":{"begin":120,"end":129},"obj":"Body_part"},{"id":"T105","span":{"begin":160,"end":168},"obj":"Body_part"},{"id":"T106","span":{"begin":309,"end":313},"obj":"Body_part"}],"attributes":[{"id":"A103","pred":"fma_id","subj":"T103","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A104","pred":"fma_id","subj":"T104","obj":"http://purl.org/sig/ont/fma/fma82755"},{"id":"A105","pred":"fma_id","subj":"T105","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A106","pred":"fma_id","subj":"T106","obj":"http://purl.org/sig/ont/fma/fma67122"},{"subj":"T103","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T104","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T105","pred":"source","obj":"LitCovid-PD-FMA-UBERON"},{"subj":"T106","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"1601","span":{"begin":120,"end":129},"obj":"Chemical"}],"attributes":[{"id":"A1601","pred":"tao:has_database_id","subj":"1601","obj":"MESH:D006639"},{"subj":"1601","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T100","span":{"begin":0,"end":8},"obj":"Disease"},{"id":"T101","span":{"begin":258,"end":266},"obj":"Disease"}],"attributes":[{"id":"A100","pred":"mondo_id","subj":"T100","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A101","pred":"mondo_id","subj":"T101","obj":"http://purl.obolibrary.org/obo/MONDO_0002595"},{"subj":"T100","pred":"source","obj":"LitCovid-PD-MONDO"},{"subj":"T101","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T442","span":{"begin":19,"end":24},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T443","span":{"begin":102,"end":103},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T444","span":{"begin":267,"end":272},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"attributes":[{"subj":"T442","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T443","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T444","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T77784","span":{"begin":120,"end":129},"obj":"Chemical"},{"id":"T949","span":{"begin":160,"end":168},"obj":"Chemical"}],"attributes":[{"id":"A948","pred":"chebi_id","subj":"T77784","obj":"http://purl.obolibrary.org/obo/CHEBI_27570"},{"id":"A949","pred":"chebi_id","subj":"T949","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"subj":"T77784","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T949","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"2_test","denotations":[{"id":"32563813-17259217-29105596","span":{"begin":250,"end":252},"obj":"17259217"}],"attributes":[{"subj":"32563813-17259217-29105596","pred":"source","obj":"2_test"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T559","span":{"begin":0,"end":155},"obj":"Sentence"},{"id":"T560","span":{"begin":156,"end":257},"obj":"Sentence"},{"id":"T561","span":{"begin":258,"end":385},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T559","pred":"source","obj":"LitCovid-sentences"},{"subj":"T560","pred":"source","obj":"LitCovid-sentences"},{"subj":"T561","pred":"source","obj":"LitCovid-sentences"}]},{"project":"MyTest","denotations":[{"id":"32563813-17259217-29105596","span":{"begin":250,"end":252},"obj":"17259217"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"attributes":[{"subj":"32563813-17259217-29105596","pred":"source","obj":"MyTest"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PMC-OGER-BB","color":"#93ece2","default":true},{"id":"LitCovid-PD-FMA-UBERON","color":"#ecc893"},{"id":"LitCovid-PubTator","color":"#ae93ec"},{"id":"LitCovid-PD-MONDO","color":"#93ec94"},{"id":"LitCovid-PD-CLO","color":"#ec93ab"},{"id":"LitCovid-PD-CHEBI","color":"#93c5ec"},{"id":"2_test","color":"#e0ec93"},{"id":"LitCovid-sentences","color":"#de93ec"},{"id":"MyTest","color":"#93ecc4"}]}]}}