PMC:7291971 / 85023-85465 JSONTXT

{"project":"LitCovid-PMC-OGER-BB","denotations":[{"id":"T1683","span":{"begin":5,"end":15},"obj":"GO:0010467"},{"id":"T1684","span":{"begin":57,"end":65},"obj":"SP_10"},{"id":"T1685","span":{"begin":66,"end":71},"obj":"PR:000000125"},{"id":"T1686","span":{"begin":76,"end":81},"obj":"SP_6;NCBITaxon:9606"},{"id":"T1687","span":{"begin":203,"end":211},"obj":"SO:0000155"},{"id":"T1688","span":{"begin":231,"end":240},"obj":"GO:0010467"},{"id":"T1689","span":{"begin":244,"end":251},"obj":"NCBITaxon:562"},{"id":"T1690","span":{"begin":315,"end":323},"obj":"NCBITaxon:7742"},{"id":"T1691","span":{"begin":324,"end":329},"obj":"NCBITaxon:10239"},{"id":"T1692","span":{"begin":353,"end":360},"obj":"GO:0032991"},{"id":"T42","span":{"begin":349,"end":374},"obj":"NCBITaxon:10239"},{"id":"T78244","span":{"begin":5,"end":15},"obj":"GO:0010467"},{"id":"T24440","span":{"begin":57,"end":65},"obj":"SP_10"},{"id":"T39744","span":{"begin":66,"end":71},"obj":"PR:000000125"},{"id":"T11149","span":{"begin":76,"end":81},"obj":"SP_6;NCBITaxon:9606"},{"id":"T17814","span":{"begin":203,"end":211},"obj":"SO:0000155"},{"id":"T90318","span":{"begin":231,"end":240},"obj":"GO:0010467"},{"id":"T4563","span":{"begin":244,"end":251},"obj":"NCBITaxon:562"},{"id":"T38822","span":{"begin":315,"end":323},"obj":"NCBITaxon:7742"},{"id":"T96822","span":{"begin":324,"end":329},"obj":"NCBITaxon:10239"},{"id":"T77113","span":{"begin":353,"end":360},"obj":"GO:0032991"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T102","span":{"begin":48,"end":56},"obj":"Body_part"},{"id":"T103","span":{"begin":82,"end":85},"obj":"Body_part"},{"id":"T104","span":{"begin":177,"end":186},"obj":"Body_part"},{"id":"T105","span":{"begin":217,"end":225},"obj":"Body_part"},{"id":"T106","span":{"begin":366,"end":370},"obj":"Body_part"}],"attributes":[{"id":"A102","pred":"fma_id","subj":"T102","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A103","pred":"fma_id","subj":"T103","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A104","pred":"fma_id","subj":"T104","obj":"http://purl.org/sig/ont/fma/fma82755"},{"id":"A105","pred":"fma_id","subj":"T105","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A106","pred":"fma_id","subj":"T106","obj":"http://purl.org/sig/ont/fma/fma67122"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"1601","span":{"begin":177,"end":186},"obj":"Chemical"}],"attributes":[{"id":"A1601","pred":"tao:has_database_id","subj":"1601","obj":"MESH:D006639"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T100","span":{"begin":57,"end":65},"obj":"Disease"},{"id":"T101","span":{"begin":315,"end":323},"obj":"Disease"}],"attributes":[{"id":"A100","pred":"mondo_id","subj":"T100","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A101","pred":"mondo_id","subj":"T101","obj":"http://purl.obolibrary.org/obo/MONDO_0002595"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T442","span":{"begin":76,"end":81},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_9606"},{"id":"T443","span":{"begin":159,"end":160},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T444","span":{"begin":324,"end":329},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T947","span":{"begin":48,"end":56},"obj":"Chemical"},{"id":"T77784","span":{"begin":177,"end":186},"obj":"Chemical"},{"id":"T949","span":{"begin":217,"end":225},"obj":"Chemical"}],"attributes":[{"id":"A947","pred":"chebi_id","subj":"T947","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A948","pred":"chebi_id","subj":"T77784","obj":"http://purl.obolibrary.org/obo/CHEBI_27570"},{"id":"A949","pred":"chebi_id","subj":"T949","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"2_test","denotations":[{"id":"32563813-17259217-29105596","span":{"begin":307,"end":309},"obj":"17259217"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T558","span":{"begin":0,"end":56},"obj":"Sentence"},{"id":"T559","span":{"begin":57,"end":212},"obj":"Sentence"},{"id":"T560","span":{"begin":213,"end":314},"obj":"Sentence"},{"id":"T561","span":{"begin":315,"end":442},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}

{"project":"MyTest","denotations":[{"id":"32563813-17259217-29105596","span":{"begin":307,"end":309},"obj":"17259217"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"4.2 Expression and purification of recombinant proteins\nSARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs)."}