PMC:7291971 / 85023-85465 JSONTXT

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    LitCovid-PMC-OGER-BB

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    LitCovid-PD-FMA-UBERON

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    LitCovid-PubTator

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    LitCovid-PD-MONDO

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    LitCovid-PD-CLO

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    LitCovid-PD-CHEBI

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    2_test

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    LitCovid-sentences

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).

    MyTest

    TextAE
    4.2 Expression and purification of recombinant proteins SARS-CoV nsp14 and human RNA N7-methyltransferase (hRNMT) coding sequences were cloned in fusion with a N-terminus hexa-histidine tag in Gateway® plasmids. The proteins were expressed in E. coli and purified following previously described protocols [33,39]. Vaccinia virus capping enzyme (D1/D12 complex) and mRNA Cap 2′-O-methyltransferase (VP39) were purchased (New England Biolabs).